Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2011.488.497AcoureneS. DjafriK. AmmoucheA. AmouracheL. DjiddaA. TamaM. TalebB. 62011106The aim of the present study was to investigate the potential of date wastes as substrate for the production of Bakers yeast and Citric acid using strains of Saccharomyces cerevisiae ATCC 1102 and Aspergillus niger ATCC 16404 and the determination of optimized production conditions. Submerged fermentations was carried out in a fermentor of 3 litters capacity. So, the obtained results show that the optimal Bakers yeast production was obtained at dilution rate of 0.22 h-1. On the other hand, the strain of Sacccharomyces cerevisiae SC-DB-A12 produced high yield biomass compared to other strains. Also, the use of ammonium phosphate provides better biomass yields compared to other nitrogen source. Concerning the citric acid production, the optimal fermentation period was 144 h. The maximum citric acid production was obtained at 30°C and at 150.0 g L-1 of sugars. In the same way, the addition of methanol at concentration up to 3.0% resulted in a marked increase in the citric acid production. In addition, the optimum pH for maximum citric acid production was 3.5. The aeration rate kept at a level of 1.0 L/L/min was found to be optimum. Finally, the best results were observed when 2.5 g L-1 ammonium nitrate and 2.5 g L-1 of potassium phosphate were added into the medium. In summary, a maximum citric acid production i.e., 126.4 g L-1 was obtained at these optimal conditions. It is concluded from these results that the date wastes could serve as a potential substrate for Baker yeast and Citric acid production.]]>Akinyemi, O.P., E. Betiku and B.O. Solomon,2003Saccharomyces cerevisiae DSM 2155 grown on glucose in fed-batch fermentation process.]]>296103Ali, S., Ikram-ul-Haq, M.A. Qadeer and J. Iqbal,2002Aspergillus niger using cane molasses in a stirred fermentor.]]>5114125Ali, S., Asad-ur-Rehman and Ikram-ul-Haq,2003Aspergillus niger in stationary culture.]]>6331333Al-Obaidi, Z.S., G.M. Aziz, T.S. Al-Hakkah and M.A. Al-Hilli,198756478Arzumanov, T.E., N.V. Shishkanova and T.V. Finogenova, 2000Yarrowia lipolytica repeat-batch culture on ethanol. ]]>53525529Asad-ur-Rehman, S. Ali and Ikram-ul-Haq,2002Aspergillus niger.]]>1108110Asad-ur-Rehman, A. Sikander and Ikram-ul-Haq,2003Aspergillus niger mutant UV-M9 on semi-pilot scale.]]>612471249Crolla, A. and K.J. Kennedy,2001Candida lipolytica Y-1095 using n-paraffin.]]>892740Daramola, M.O. and L. Zampraka,2008Sacharomyces cerevisiae in fed batch fermentor.]]>711071114De Kock, S.H., J.C. du Preez and S.G. Kilian,2000Saccharomyces cerevisiae strains in aerobic chemostat cultures.]]>246231236Demirel, G., O.Y. Kursat and A.Yasar, 2005Aspergillus niger A-9 and investigations of its various effects.]]>89393396Drysdale, C.R. and M.H. McKay,1995Aspergillus niger in surface culture on inulin.]]>20252254Ejiofor, A.O., Y. Chisti and M. Moo-Young,1996Pennisetum typhoides) flour hydrolysate as the carbon source.]]>16102109El-Holi, M.A. and K. Al-Delaimy,2003Aspergillus niger.]]>2356359Franz, A., W. Burgstaller, B. Muller and F. Schinner,1993Penicillium simplicissimum.]]>13921012107Gueguim-Kana, E.B., J.K. Oloke and A. Lateef,2007Saccharomyces cerevisiae DS2155, using glucose limited exponential fed batch cultures with variable specific growth rates.]]>611221127Ikram-ul-Haq, A. Sikander, M.A. Qadeer and J. Iqbal,2002Aspergillus niger GCMC-7 using molasses based medium.]]>5125132Ikram-ul-Haq, S. Ali, M.A. Qadeer and J. Iqbal,200386227233Jorgensen, H., L. Olsson, B. Ronnow and E.A. Palmqvist,200259310317Mattey, M.,19921287132Mazhar, R., S. Ali, Ikram-ul-haq and A. Waheed,20033360370Miskiewicz, T. and D. Borowiak,20058113Mohammed, N.A., Z.S. Al-Obaidi, N.A. Hassen and M.A. Jassem,198612045Papagianni, M., F. Wayman and M. Mattey,2005Aspergillus niger.]]>7171787186Pazouki, M., P.A. Felse, J. Sinha and T. Panda,2000Aspergillus niger and Candida lipolytic using molasses and glucose. ]]>22353361Reed, G. and H.J. Peppler,19731973pp: 42-94pp: 42-94Roukas, T., 1998Aspergillus niger atcc 9124.]]>1291104Sanjay, K. and P. Sharma,199423211217Shashi, B. and V.K. Joshi,2006657276Vandenberghe, L.P.S., C.R. Soccol, A. Pandey and J.M. Lebeault,199942263276Van Hoek, P., E. de Hulster, J. van Dijken and J.T. Pronk,200068517523Walid, A.L., M.G. Khaled and R.H. Ehab,2006Aspergillus niger isolate: Optimization of process parameters through statistical experimental designs.]]>19171173Ejiofor, A.O., B.O. Solomon, C. Posten and W.D. Deckwer,1994 cerevisiae on glucose in a fed-batch fermentation strategy for accurate parameter estimation.]]>41664669Ministry of Agriculture,20062006pp: 42-44pp: 42-44Papagianni, M.,2007Aspergillus niger: Biochemical aspects, membrane transport and modeling.]]>25244263