Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2011.215.219in vitro Seed Germination of Ativisha (Aconitum heterophyllum Wall) of Uttarakhand]]>SrivastavaNidhi SharmaVikas DobriyalAnoop K.KamalBarkha GuptaSanjay JadonVikas S.22011102In the present study in-vitro seed germination protocol using various pre-sowing treatments for Aconitum heterophyllum Wall intended for its ex-situ conservation was optimized. Temperature requirement for seed germination in this species was optimized under controlled conditions which revealed that low temperature of 15°C (p<1.0%) was obligatory. Two in-vitro presowing treatments: PST-1 (pre-treated seeds kept at normal room temperature) and PST-2 (pre-treated seeds kept at 15°C) were also studied. Highest seed germination was recorded with PST-2 (p<0.1%) with 0.5 mg L-1 IAA (97.17%) as compared to PST-1 (p<1.0%). As seed embryo in A. heterophyllum Wall is small, it requires low temperature to grow and also GA3 (100 and 200 μM) could not improve the seed germination even at low temperature; so it may be assumed that it exhibit deep complex morphophysiological dormancy (MPD).]]>Ahmad, M., W. Ahmad, M. Ahmad, M. Zeeshan, Obaidullah and F. Shaheen,2008Aconitum heterophyllum Wall with antibacterial activity.]]>2310181022Ameri, A.,1998Aconitum alkaloids on the central nervous system.]]>56211235Anwar, S., B. Ahmad, M. Subhan, W. Gul and Nazar-ul-Islam,2003Aconitum chasmanthum.]]>3989993Bao, J.P. and S.L. Zhang,2010Pyrus betulaefolia Bge. and Pyrus calleryana Dcne).]]>38348357Baskin, C.C. and J.M. Baskin,1994Delphinium tricorne (Ranunculaceae).]]>155738743Baskin, C.C. and J.M. Baskin,19983rd Edn.,Pages: 666Pages: 666Beigh, S.Y., I.A. Nawchoo and M. Iqbal,2005Aconitum heterophyllum: A Critically Endangered Medicinal Herb of the Northwest Himalayas.]]>14756Bewley, J.D.,1997910551066CAMP.,20032003Forbis, T.A., S.K. Floyd and A. DeQueiroz,20025621122125Frost, C.,1974Eranthis hiemalis and its relation to giberrallic acid.]]>30200205Giri, A., P.S. Ahuja and P.V.A. Kumar,1993Aconitum heterophyllum Wall.]]>32213218Grushvitzky, I.V.,19671967336336Hilhorst, H.W.M. and C.M. Karssen,199211225238Iglesias, R.G. and M.J. Babiano,1997100500504IUCN,1993Jabeen, N., A.S. Shawl, G.H. Dar, A. Jan and P. Sultan,2006Aconitum heterophyllum- medicinal plant.]]>5287291Nisar, M., M. Ahmad, N. Wadood, M.A. Lodhi, F. Shaheen and M.I. Choudhary,2009Aconitum heterophyllum wall: Selective butyrylcholinestrase inhibitors.]]>244751Murashige, T. and F. Skoog,196215473497Nautiyal, B.P., M.C. Nautiyal, V.P. Khanduri and N. Rawat,2009Aconitum heterophyllum Wall: A critically endangered alpine medicinal plants of Himalaya, India.]]>331320Nautiyal, B.P., V. Prakash, R. Bahuguna, U.C. Maithani, H. Bisht and M.C. Nautiyal,2002Aconite species in Garhwal Himalaya.]]>43297303Pandey, H., S.K. Nandi, M. Nadeem and L.M.S. Palni,2000Aconitum heterophyllum Wall. and A. balfourii Stapf.: Important Himalayan species of medicinal value.]]>283948Pandey, S., R. Khushwaha, O. Prakash, A. Bhattacharya and P.S. Ahuja,2005Aconitum heterophyllum Wall, an endangered medicinal plant of the Himalaya through mass propagation and its effect on growth and alkaloid content.]]>3127135Pradhan, B.K. and H.K. Badola,2010ex situ produced seeds in Swertia chirayita, a critically endangered medicinal herb.]]>3139149Sen, S.,20105122132Senel, E., Y. Ozdener and D. Incedere,20073 on the germination of Verbascum bithynicum, Verbascum wiedemannianum and Salvia dicroantha.]]>1011181121Srivastava, N., V. Sharma, B. Kamal, A.K. Dobriyal and V.S. Jadon,2010Aconitum heterophyllum wall-endangered medicinal species.]]>5414419Srivastava, N., V. Sharma, B. Kamal, A.K. Dobriyal and V.S. Jadon,2010Aconitum sp. (Ranunculaceae) under different area: A review.]]>9411427Vandelook, F., J. Lenaerts and A.V.A. Jozef,2009Aconitum lycoctonum L.]]>204536542Walck, J.L., C.C. Baskin and J.M. Baskin,1999Thalictrum mirabile (Ranunculaceae) require cold stratification for loss of non-deep simple morphophysiological dormancy.]]>7717691776Srivastava, N., B. Kamal, V. Sharma, Y.K. Negi, A.K. Dobriyal, S. Gupta and V.S. Jadon,2010Aconitum heterophyllum-An endangered medicinal herb.]]>23742