Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2011.203.208Agrobacterium rhizogenes on Enhancing Transformation Frequency in Host Plants]]>ChandranR. PratapPottyV. P.22011102The aim of the present investigation was to find the best Agrobacterium rhizogenes strain, which can induce hairy root initiation faster and the best inducer molecule which can potentiate hairy root induction in host plants. Four different wild strains of A. rhizogenes, namely A. rhizogenes 15834, A4, WS and WR were used for hairy root infection in four host plants (Ipomoea batatas, Solenostemon rotundifolius, Vigna vexillata and Canavalia sp). Agrobacterium rhizogenes strains initiated hairy roots from the host plants without inducer molecules (AS and sugars) to a lesser level. Phenolic compound acetosyringone (AS) and sugars (D-glucose, mannose and galactose) were used to activate the virulence genes of the root inducing (Ri) plasmid which initiates transcription of virulence genes for hairy root induction. AS and sugars activated the virulence genes of the root inducing (Ri) plasmid of A. rhizogenes strains, which further initiated the transfer of T-DNA region to the host plants and enhanced hairy root induction frequency. As enhanced hairy root induction percentage in the entire host plants ranges from 11 to 61.5% and sugars from 16.5 to 39.5%. A. rhizogenes 15834 was found to initiate hairy roots early when compared to other strains of A. rhizogenes.]]>Bond, J.E. and M.L. Roose,199819229234Cangelosi, G.A., R.G. Ankenbauer and E.W. Nester,1990Agrobacterium virulence genes through a periplasmic binding protein and a transmembrane signal protein.]]>8767086712Chandran, R.P. and V.P. Potty,2008Agrobacterium rhizogenes on five host plants.]]>7122128Citovsky, V., J. Zupan, D. Warnick and P. Zambryski,1992Agrobacterium Vir E2 protein in plant cells.]]>25618021805Danesh, Y.R., E.M. Goltapeh, A. Alizadeh and M.M. Sanavy, 200668791David, C. and J. Tempe,1988Brassica oleracea L. var. cotrytis) by Agrobacterium rhizogenes.]]>78891Delmotte, F.M., D. Delay, J. Cizeau, B. Guerin and J.C. Leple,1991Agrobacterium vir-inducing activities of glycosylated acetosyringone, acetovanillone, syringaldehyde and syringic acid derivatives.]]>3035493552Giri, A., S.T. Ravindra, V. Dhingra and M.L. Narasu,2001Agrobacterium rhizogenes in induction of hairy roots and artemisinin production in Artemisia annua.]]>81378382Guillon, S., J. Tremouillaux-Guiller, P.K. Pati, M. Ridean and P. Gantet,200693341346Hansen, J., J.E. Jorgensen, J. Stougaard and K.A. Marcker,198981215Hsu, C.L. and W.C. Lee,20103915971604Huang, J.Q., Z.M. Wei, H.L. An and Y.X. Zhu,2001Agrobacterium tumefacien mediated transformation of rice with the spider insecticidal gene conferring resistance to leaf folder and striped stem borer.]]>11149155Jin, S., Y.N. Song, W.Y. Deng, M.P. Gordon and E.W. Nester,1993Agrobacterium tumefacien does not function at elevated temperatures.]]>17568306835Kumar, V., A. Sharma, B.C.N. Prasad, H.B. Gururaj and G.A. Ravishankar,20069349357Levee, V., E. Garin, K. Klimaszewska and A. Seguin,1999Pinus strobes L.) after co-cultivation of embryogenic tissues with Agrobacterium tumefaciens.]]>5429440Ming, O.C., A.S. Wen, U. Sinniah, R. Xavier and S. Subramaniam,2007Rosa hybrida L.) cv. nikita.]]>2387397Nguyen, C., F. Bourgaud, P. Forlot and A. Guckert,1992Psoralea species.]]>11442447Piers, K.L., J.D. Heath, X. Liang, K.M. Stephens and E.W. Nester,1996Agrobacterium tumefacien mediated transformation of yeast.]]>9316131618Rahimi, K., K. Haghbeen, J. Marefatjo, F.R. Jazii and R. Sheikhani,2008Valeriana sisymbriifolium by agrobacterium rhizogenes.]]>7200204Raveendar, S. and S. Ignacimuthu,2010Agrobacterium mediated transformation in cowpea Vigna unguiculata L. walp.]]>91256263Gao, R. and D.G. Lynn,2005Agrobacterium pathogenesis.]]>18721822189Shanks, J.V. and J. Morgan,199910151155Shimoda, N., A. Toyoda-Yamamoto, J. Nagamine, S. Usami, M. Katayama, Y. Sakagami and Y. Machida,1990Agrobacterium tumefacien genes by synergistic actions of phenolic signal molecules and monosaccharide.]]>8766846688Tao, J. and L. Li,2006Torenia fournieri L. mediated by Agrobacterium rhizogenes.]]>72211216Vanhala, L., R. Hiltunen and K.M. Oksman-Caldentey,1995Agrobacterium strains on hairy root formation of Hyoscyamus muticus.]]>14236240Winans, S.C.,1992Agrobacterium-plant interactions.]]>561231Van Wordragen, M.F. and H.J.M. Dons,1992Agrobacterium tumefaciens-mediated transformation of recalcitrant crops.]]>101236Wang, Y.M., J.B. Wang, D. Luo and J.F. Jia,2001Agrobacterium rhizogenes on Alhagi pseudoalhagi.]]>11279284Gelvin, S.B.,2003Agrobacterium mediated plant transformation: The biology behind the gene-jockeying tool.]]>671637