Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2010.170.175SalehN. IbrahimM.A. ArchoukiehE. MakkiyaA. Al-ObaidiM. AlobydiH. 2201092The aim of this study is to ascertain the possible application of Random Amplification of Polymorphic DNA (RAPD) analysis as a genetic test to investigate DNA polymorphisms and detection of genomic markers in various types of leukemia. The results showed unique profiles of amplified DNA fragments produced in genomic DNA of three types of leukemia by an arbitrary primer of decamer oligonucleotides OPA-09. The primer produced four types of amplified DNA fragments (980, 1659, 2187 and 3162 bp). The smallest amplified DNA fragment (980 bp) appeared in 14.3 and 13.3% of tested acute myeloid leukemia and chronic myeloid leukemia patients, respectively; but was absent in genomic DNA of chronic lymphoid leukemia and normal individuals. Whereas the largest amplified fragment (3162 bp) was present in 12.5, 20 and 75% of chronic lymphoid leukemia, chronic myeloid leukemia and normal individuals, respectively and was absent in acute myeloid leukemia. On the other hand, the two amplified fragments (1659 and 2187 bp) were present in normal and leukemia patients. Cluster analysis of amplified DNA fragments grouped the leukemia patients in two main groups. The detected DNA polymorphisms by the arbitrary primer OPA-09 might find application in developing efficient RAPD primer for diagnosis of leukemia.]]>Awamleh, H., D. Hassawi, H. Migdadi and M. Brake, 2009Punica granatum L. landraces grown in jordan using amplified fragment length polymorphism markers.]]>8316322Awasthi, A.K., G.M. Nagaraja, G.V. Naik, S. Kanginakudru, K. Thangavelu and J. Nagaraju,2004Morus) as revealed by RAPD and ISSR marker assays.]]>519Bolufer, P., E. Barragan, M. Collado, J. Cervera, J.A. Lopez and M.A. Sanz,20063014711491Misra, D.A.A., I.M. Sulaiman, S. Sinha, C. Sarkar, A.K. Mahapatra and S.E. Hasnain,19982064548Goossens, W., V.V. Duppen and R.L. Verwilghen,199113291395Gu, W., C.M. Post, G.D. Aguirre and K. Ray,1999909698Haferlach, T., W. Kern, S. Schnittger and C. Schoch,2005Leukemias.]]>56223234Maeda, T., A. Jikko, H. Hiranuma and H. Fuchihata,1999138183188Mollah, M.B.R., M.S. Alam, F.B. Islam and M.A. Ali,200547375Mollah, M.B.R., F.B. Islam, M.S. Islam, M.A. Ali and M.S. Alam,20098462467Odero, M.D., J.L. Soto, E. Matutes, J.I.M. Subero and I. Zudaire et al.,2001130813Ong, T.M., B. Song, H.W. Qian, T.W. Qian, Z.L. Wu and W.Z. Whong,199819233235Papadopoulos, S., T. Benter, G. Anastassiou, M. Pape and S. Gerhard et al.,200299193200Saiki, R., D.H. Gelfand, S. Stoffel, S.J. Scharf and R. Higuchi et al.,1988239487491Sambrook, J. and D.W. Russell,20013rd Edn.,Pages: 2344Pages: 2344Scarra, A., P.S. Moore, G. Rigaud and F. Menestrina,2001414753Seufi, A.M., S.S. Ibrahim, T.K. Elmaghraby and E.E. Hafez,2009288080Singh, K.P. and D. Roy,20012693343Sood, A.K. and R.E. Buller,19961324992504Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey,19901865316535Xian, Z.H., W.M. Cong, S.H. Zhang and H.C. Wu,20051141024107Yamamoto, K., R. Murakami and Y. Takamura,2001169199Zhang, S.H., W.M. Cong, Z.H. Xian, H. Dong and M.C. Wu,2004Hepatocelluar carcinoma revealed by using random amplified polymorphic DNA method.]]>130757761Ibrahim, M.A., N. Saleh, E. Archoukieh, H.W. Al-Obaide, M.M. Al-Obaidi and H.M. Said,20102010