Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2009.53.61Bacillus subtilis Against Certain Plant Pathogenic Fungi, I]]>M. MatarS. A. El-KazzazS. E. WagihE. El-DiwanyA.I. E. MoustafaH. A. Abo-ZaidG. Abd-ElsalamH.E. E. HafezE. 1200981The antagonistic and inhibitory activity of fourteen Bacillus subtilis isolates (B1 to B14) obtained from different Egyptian sites, were tested against six fungal isolates belonging to four different genera, Rhizoctonia solani, Helminthosporium spp., Alternaria spp. and Fusarium oxysporum. Cultural, morphological and physiological characteristics of these isolates were found to be identical to Bacillus subtilis. When the fourteen B. subtilis isolates were tested as biological control agents for their antagonistic effect on the in vitro growth of the fungal isolates, four B. subtilis isolates B1, B4, B7, B8 had more antagonistic effect on all fungal isolates. Supernatant of B. subtilis isolate B7 had antagonistic effect on 6 fungal isolates but it was more effective on Helminthosporium spp., Alternaria spp. and F. oxysporum.B. subtilis as well as, isolate B7 showed effectiveness in reducing disease incidence and severity levels of tomato plants when added to the F. oxysporum and R. solani-infested soil. Also, it stimulated the growth of tomato plants compared to the other. HPLC analysis of the HCl precipitate of B. subtilis isolate B7 culture supernatant revealed that an identical pattern of five peaks to that of a purified preparation of iturin A was obtained.]]>Asaka, O. and M. Shoda,1996Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14.]]>6240814085Bais, H.P., R. Fall and J.M. Vivanco,2004Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production.]]>134307319Cavaglieri, L., J. Orlando, M.I. Rodriguez, S. Chulze and M. Etcheverry,2005Bacillus subtilis against Fusarium verticillioidesin vitro and at the maize root level.]]>156748754Chitarra, G.S., P. Breeuwer, M.J. Nout, A.C. van Aelst , F.M. Rombouts and T. Abee,2003Bacillus subtilis YM 10-20 inhibits germination of Penicillium roqueforti conidiospores.]]>94159166Gong, M., J. Wang, J. Zhang, H. Yang and X. Lu et al.,2006Bacillus subtilis strain PY-1 in vitro and identification of its antifungal substance (iturin A).]]>38233240Jiang, Y.M., X.R. Zhu and Y.B. Li,2001Bacillus subtilis.]]>34430436Kita, N., T. Ohya, H. Uekusa, K. Nomura and M. Manago et al.,2005Phomopsis root rot by Bacillus subtilis RB14-C.]]>39109114Marten, P., S. Bruckner and P. Luth,1999Bacillus subtilis strain B2g.]]>1067481Raaijmakers, J.M., M. Vlami and J.T. de Souza,200281537547Sharga, B.M. and G.D. Lyon,1998Bacillus subtilis BS 107 as an antagonist of potato blackleg and soft rot bacteria.]]>44777783Sneath, P.H.A., N.S. Mair, M.E. Sharpe and J.G. Holt,19861st Edn.,pp: 1104-1139pp: 1104-1139Toure, Y., M. Ongena, P. Jacques, A. Guiro and P. Thonart,2004Bacillus subtilis GA1 in the reduction of grey mould disease caused by Botrytis cinerea on apple.]]>9611511160Wagih, E.E., M.R.A. Shehata, S.A. Farag and M.K. Dawood,1989Dracaena sanderiana in Egypt.]]>126716Walker, R., A.A. Powell and B. Seddon,1998Bacillus isolates from the spermosphere of peas and dwarf French beans with antifungal activity against Botrytis cinerea and Pythium species.]]>84791801Zuber, P., M.M. Nakano and M.A. Mahariel,19931993pp: 897-916pp: 897-916Collee, J.G., A.G. Fraser, B.P. Marmion and A. Simmons,199614th Edn.,pp: 131-149pp: 131-149