Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2009.1.12Impatiens balsamina, Mediated by Microprojectile Bombardment]]>TahaA.M. WagiranA. GhazaliH. HuyopF. ParveezG.K.A. 1200981In this study, a transformation system was developed
by initially optimizing the microprojectile bombardment parameters for
cotyledonous explants. The parameters optimized were combination of distance
from stopping screen to target tissue and helium pressure, number of bombardment,
preculture duration prior to bombardment, DNA amount, osmoticum treatment
and post-bombardment incubation time. Determination of minimal inhibitory
concentration for efficient selection of transformants was also carried
out for hygromycin. Different concentrations of hygromycin (25, 50, 75
and 100 mg L-1) were tested against different ages of explants
(0, 1, 3 and 5 weeks) for efficient selection of transformants. Using
the optimized parameters, transformation of cotyledons was carried out
followed by selection on 75 mg L-1 hygromycin at different
weeks of post-bombardment. Transgenic plants were successfully regenerated
from bombarded explants and confirmed via histochemical GUS assay and
PCR analysis.]]>Altpeter, F., N. Baisakh, R. Beachy, R. Bock and T. Capell et al.,200515305327Aragao, F.J.L., M.F. Grossi de Sa, M.R. Davey, A.C.M. Brasileiro, J.C. Faria and E.L. Rech,1993Phaseolus vulgaris L.) using an electrical particle acceleration device.]]>12483490Batista, D., S. Fonseca and S. Serrazina,2008Humulus lupulus L.) var. Eroica by particle bombardment.]]>2711851196Birch, R.G. and R. Bower,19941994pp: 3-37pp: 3-37Birch, R.G.,199748297326Christou, P., D.M. McCabe, B.J. Martinell and W.F. Swain,19908145151Clemente, T.E., D. Robertson, T.G. Isleib, M.K. Beute and A.K. Weissinger,1992Arachis hypogaea L. leaflets from mature zygotic embryos as recipient tissue for biolistic gene transfer.]]>1275284Daughtrey, M.L., R.K. Jones, J.W. Moyer and M.E. Daub,19978112201230Doyle, J.J. and J.L. Doyle,1987191115Gao, C., L. Jiang, M. Folling, L. Han and K.K. Nielsen,2006Poa pratensis L.) plants following biolistic gene transfer.]]>251925Ghosh, M., T. Saha, P. Nayak and S. Sen,2002Corchorus capsularis L.]]>20936942Gobert, A., G. Park, A. Amtmann, D. Sanders and F.J. Maathuis,2006Arabidopsis thaliana cyclic nucleotide gated channel 3 forms a non selective ion transporter involved in germination and cation transport.]]>57791800Imaseki,19861986pp: 249-264pp: 249-264Indurker, S., H.S. Misra and S. Eapen,2007Cicer arietinum L.) with insecticidal crystal protein gene using particle gun bombardment.]]>26755763Jain, R.K., S. Jain, B. Wang and R. Wu,1996Basmati rice plants.]]>15963968Jain, M., K. Chengalrayan, A. Abouzid and M. Gallo,2007Saccharum spp. hybrid) plants.]]>26581590Jefferson, R.A., T.A. Kavanagh and M.W. Bevan,1987639013907Kikkert, J.R., J.R. Vidal and B.I. Reisch,20042866178King, S.P. and K.J. Kasha,1994Hordeum vulgare L.) immature embryos.]]>30P117123Klein, T.M., E.C. Harper, Z. Svab, J.C. Sanford, M.E. Fromm and P. Maliga,1988Nicotiana cells by the particle bombardment process.]]>8585028505Latha, A.M., K.V. Rao, T.P. Reddy and V.D. Reddy,2006Pennisetum glaucum (L.) R. Br.) plants resistant to downy mildew.]]>25927935Lee, D.G., S.Y. Shin, D.H. Kim, M.Y. Seo, J.H. Kang, Y.H. Lee, K.L. Kim and K.S. Hahm,1999Impatiens balsamina against Candida albicans.]]>2110471050Lee, S.I., Y.G. Shon, C.Y. Kim, C.O. Lim, Y.J. Choi, H.I. Kim, S.Y. Lee and S.H. Lee,20035163165Li, L., R. Qu, A. Kochko, C. Fauquet and R.N. Beachy,199312250255Li, D., K. Zhao, B. Xie, B. Zhang and K. Luo,2003Capsicum anuum L.]]>21785788Men, S., X. Ming, Y. Wang, R. Liu, C. Wei and Y. Li,200321592598Murashige, T. and F. Skoog,196215473497Parveez, G.K.A., M.K.U. Chowdhury and N.M. Saleh,1996Elaeis guineensis Jacq. transformation.]]>4219228Parveez, G.K.A. and P. Christou,1998Elaeis guineensis Jacq embryogenic callus cultures.]]>102938Parveez, G.K.A.,2000Elaeis guineensis Jacq.) using Biolistic Techniques.]]>2000pp: 327-350pp: 327-350Perl, A., H. Kless, A. Blumenthal, G. Galili and E. Galun,1992235279284Ritala, A., L. Mannonen, K. Aspegren, M. Salmenkallio-Marttila, U. Kurten, R. Hannus, J.M. Lozano, T.H. Teeri and V. Kauppinen,199312435440Russell, J.A., M.K. Roy and J.C. Sanford,19929810501056Sailaja, M., M. Tarakeswari and M. Sujatha,2008Ricinus communis L.) via particle gun-mediated gene transfer using embryo axes from mature seeds.]]>2715091519Sanford, J.C., F.D. Smith and J.A. Russell,1993217483509Santos, M.O., V.S.A. Barros, M.L.P. Tinoco and F.J.L. Aragoa,200247176Taylor, M.G. and I.K. Vasil,1991Pennisetum glaucum (L.) R. Br.) embryos following microprojectile bombardment.]]>10120125Wong, J.R.,19941994pp: 46-51pp: 46-51Ye, X., S.K. Brown, R. Scorza, J. Cordts and J.C. Sanford,1994119367373