Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2008.80.86In vitro Selection for Resistance Against Purple Blotch Disease of Onion (Allium cepa L.) Caused by Alternaria porri]]>TripathiM.K. TiwariS. KhareU.K. 1200871Disease tolerant/resistant onion (Allium cepa L. cv ADR and ALR) cell lines were selected against purple blotch disease caused by Alternaria porri. For this purpose callus and cell suspension cultures derived from mature embryos were exposed to purified toxic culture filtrate produced by the fungus supplemented with MS culture medium. Two selection methods were used: a continuous method in which four cycles of selection were performed on toxic medium whereas during discontinuous method, a pause was given after the second and third cycle of selection using non-toxic medium. Almost 4700 calli obtained from mature embryo and 8300 cell clumps from cell suspension cultures of two onion cultivars were exposed to media with phytotoxin for selection. The discontinuous method proved to be superior as it allowed the calli to regain their regeneration capability. Continuous exposure with toxic culture filtrate resulted up to 78% mortality. In vivo pathogenesity test of regenerated plants from the surviving tolerant/resistant cell lines revealed non-sensitive against pathogen toxin. A total 4 lines of ADR and 2 of ALR were documented resistant/tolerant amongst an array of putative resistant/tolerant lines during S1 generation.]]>Ashok, J., B. Fakrudin, M.S. Kuruvinashetti and B.Y. Kullaiswami,2004Sclerotium roltsii Sacco culture filtrate treated callus.]]>64221224Behnke, M.,1979Phytophtora infestans and regeneration of plants.]]>556971Behnke, M.,1980Fusarium oxysporum.]]>85254258Chawla, H.S. and G. Wenzel,198717337343Gangaotti, B.V., K.K. Karma and S.S. Patil,1985In vitro selection of phaseolotoxin resistant plants using meristem cultures of bean.]]>7513161316Gengenbach, B.G. and C.E. Green,1975Helminthosporium maydis race T pathotoxins.]]>15645649Hartman, C.L., T.J. McCoy and T.R. Knous,1984Medicago sativa) cell lines and regeneration of plants resistant to the toxin(s) produced by Fusarium oxysporium f. sp. medicaginis.]]>34183194Murashige, T. and F. Skoog,196215473497Rudolph, K.,19761976pp: 279-315pp: 279-315Sacristan, M.D.,1985Brassica cultures.]]>1035763Schuchmann, R.,1985In vitro selection auf fusarium-resisttenz bei der Kartoffel.]]>1985Song, H.S., L.M. Lim and J.M. Clark Jr.,1994Septoria glycines.]]>83659661Sunlihua, S.J. and X. Lu,1997In vitro induction and screening of mutants resistant to X. oryzae from callus culture in rice. In: Genetic manipulations in crops.]]>215252Thanutong, P., I. Furussawa and M. Yamamoto,1983Pseudomonas and Alternaria toxins.]]>66209215Tiwari, S., M.K. Tripathi, U.K. Khare and S.K. Pathak,2004Allium cepa L.).]]>31107111Tiwari, S., M.K. Tripathi, U.K. Khare and R. Rana,2007Allium cepa L.).]]>6100106Venkatachalam, P., K. Sankara Rao, P.B. Kavi Kishor and N. Jayabalan,1998Cercosporidium personatum culture filtrate-treated callus.]]>746165Vidhyasekaran, P., D.H. Ling, E.S. Borromeo, F.J. Zapata and T.W. Mew,1990Helminthosporium oryzae toxin-resistant calluses.]]>117515523Yoder, O.C.,19831983pp: 335-353pp: 335-353