Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2008.10.18Ai-NoiSauvaphap Abd-AzizSuraini AlitheenNorjahan HassanOsman KarimMohamed Ismail Abdul 1200871The aim of the study was to optimized
cyclodextrin glycosyltransferase (CGTase) production using local isolated
strain MK6 which was identified as Bacillus sp. Optimum activity
obtained at temperature of 70 °C and the enzyme shows a wide range
of pH stability ranging from 4-10 when stored at 4 °C for 24 h and
temperature stability ranging from 30-80 °C at 1 h incubation period.
The CGTase activity was even maintained at 0.4 U mL-1 at 90
°C for 40 min incubation. Prior to optimization of CGTase production,
selection for the best carbon source through detection using modified
phenolphthalein method containing different types of starch were performed.
Sago starch gave significant result and was used for further optimization
using statistical analysis namely Response Surface Methodology (RSM) approach.
The optimal calculated values were 3.34% sago starch, initial pH of 10.15
and agitation speed of 187 rpm; with predicted activity of 2.07 U mL-1
of CGTase. These predicted optimal parameters were confirmed in the laboratory
and the final CGTase activity obtained was very close to the predicted
value of 2.56 U mL-1.]]>Abelyan, V.A., Z.G. Avakyan, A.G. Melkumyan, A.M. Balayan, L.V. Uzunyan and A.V. Gasparyan,199257430437Abelyan, V.H.,20012001Rosso, A.M., S.A. Ferrarotti, N. Krymkiewicz and B.C. Nudel, 2002Bacillus circulans DF 9R.]]>119Bart, A.V., C.M.U. Joost, W.D. Bauke and D. Lubbert,20001543336360Cao, X., Z. Jin, X. Wang and F. Chen,200538309314Gawande, B.N. and A.Y. Patkar,2001Klebsiella pneumoniae AS-22.]]>28735743Ho, K.S., S. Mamot, H. Osman, K. Kamarulzaman, A.I. Fauzi, A.R. Roshanida, N.M. Nik Azmi and M.I. Rosli,2004Bacillus sp. G1.]]>4011011111Jamuna, R., N. Saswathi, R. Sheela and S.V. Ramakrishna,1993Bacillus cereus for the production of cyclodextrins.]]>43163176Kabaivanova, L., E. Dobreva and V. Miteva,1999Bacillus stearothermophilus R2 strain isolated from a Bulgarian hot spring.]]>8610171023Kamaruddin, K., R.M. Illias, S.A. Aziz, M. Said and O. Hassan,2005Cycas revoluta) starch.]]>41117125Kaneko, T., T. Kato, N. Nakamura and K. Horikoshi,1987344548Mahat, M.K., R.M. Illias, R.A. Rahman, N.A.A. Rashid and N.A.N. Mahmood et al.,2004Bacillus sp. TS1-1: Media optimization using experimental design.]]>35467473Makela, M.J., T.K. Korpela, J. Puisto and S.V. Laakso,1988368388Montgomery, D.C.,19763rd Edn.,Nogrady, N., I. Pocsi and A. Szentirmai,1995Bacillus macerans.]]>21233243Park, S.C., H.H. Park and S.H. Kim,19895311671169Penninga, D., B. Strokopytov, H.J. Rozeboom, C.L. Lawson, B.W. Dijkstra, J. Bergsma and L. Dijkhuizen,1995Bacillus circulans strain 251 affect activity and product specificity.]]>3433683376Pocsi, I., N. Nogrady, A. Liptak and A. Szentirmai,1998Bacillus macerans.]]>437174Martins, R.F. and R. Hatti-Kaul,2002Bacillus agaradhaerens isolate: Purification and characterisation]]>30116124Stam, C.J., J.P.M. Pijn, P. Suffczynski and F.H.L. da Silva,199911018011813Suelter, C.H.,1985Thatai, A., M. Kumar and K.J. Mukherjee,1999Bacillus sp. isolated from soil.]]>293547Tien, S.F.,2001Bacillus sp. G1.]]>2001Turnes, R.E. and S. Bahar,1996Bacillus sp. in rice and corn flakes.]]>47133137