Biotechnology1682-296x1682-2978Asian Network for Scientific Information10.3923/biotech.2006.49.52In vitro Tissue Culture Techniques for Propagation of Amygdalus communis L. Cultivars, Garrigues and Yaltsinki]]>YaparHaki CanCanan OzaslanMehmet AytekinTurkan AtliHalit 1200651Almond (Amygdalus communis L.) is planted and naturally spreads in the Southeastern Anatolian region where some hybrid varieties are cultivated and the adaptation studies have still been continued. Although being easily propagated through grafting, plantation in large areas may have some problems, due to the environmental stress. Embryos, nodal segmets of mature shoots and shoot tips of two A. communis L. cultivars, Garrigues and Yaltsinki, were cultured on MS media supplemented with several concentrations and combinations of cytokinins and auxins, for in vitro micropropagation. The best root formation in embryo cultures for both cultivars was obtained in the media without growth regulators. The highest stem development was obtained with 4.0 mg L-1 of IAA in Garrigues. Callus developed from cultured shoot tips. MS media supplemented with 4.0 mg L-1BAP induced fast growing-fragile callus growth. The best nodal explant development occurred with MS contained 2.0 mg L-1 BAP and 0.5 mg L-1 IAA. Results obtained demonstrated that the nodal explants could be used for direct embryogenesis of Garrigues and Yaltsinki varieties.]]>Baraldi, R., F. Rossi and B. Lercari, 1988In vitro shoot development of Prunus GF 655-2: Interaction between light and benzyladenine.]]>74440443Borkowska, B. and J. Szczerba, 1991Prunus cerasus L. shoot cultures.]]>42911915Caboni, E., S. Speranza and C. Damiano, 1994in vitro rooting of almond.]]>85355Caboni, E., 1994in vitro rooting in an almond Prunus dulcis Mill. genotype.]]>373201205Channuntapipat, C., M. Sedgley and G. Collins, 200398473484Deogratias, J.M., F. Dosba and A. Lutz, 198911332336Dokuzo-uz, M. and R. Gulcan, 19791979Edstrom, J.P. and M. Viveros, 19961996Gulcan, R., 1976Gurel, S. and Y. Gulsen, 1998in-vitro shoot production of almond Amygdalus communis L.]]>22375379Hammerschlag, F.A., 1982in vitro.]]>178586Jones O.P., 1985in vitro Micropropagation of Temperate Fruit Trees.]]>1985Kuden, A.B., 19984702933Murashige, T. and F. Skoog,196215473497Murashige, T.,19901990pp: 3-9pp: 3-9Peterson, R.L., 19751975pp: 125-162pp: 125-162Philip, V.J. and J. Padikkala,1989Vanilla planifolia.]]>135233236Rugini, E. and D.C. Verma,1983Prunus amygdalus, Batsch) cultivar.]]>28273281Tabachnik, L. and D.E. Kester, 1977in vitro.]]>12545547Tisserat, B., 19911991pp: 79-105pp: 79-105Tran-Thanh-Van, K.,1981in vitro cultures.]]>32291311