Asian Journal of Scientific Research1992-14542077-2076Asian Network for Scientific Information10.3923/ajsr.2020.50.57Ou-yahiaDouaae Fikri-BenbrahimKawtar LekbachYassir BennounaFadoua BarkaiHassan KoraichiSaad Ibnsouda 12020131Background and Objective: The understanding of bacterial adhesion and its effect on the properties of material surfaces in seawater is the key to mitigate biofilm formation and biofouling. Therefore, the objective of this work was to evaluate the impact of several marine bacterial strains on the physico-chemical properties of stainless steel materials. Materials and Methods: We firstly investigated the adhesion ability of 6 bacterial strains isolated from seawater in the port of Nador Aït Nsar (Morocco), on 304 and 316 sea-immersed stainless steels. Secondly, we evaluated the adhesion effect of combination of all isolated strains on physico-chemical characteristics of stainless steel surfaces. Results: The molecular identification of isolated strains indicated that these strains were Pseudomonas stutzeri (KP307770.1), Bacillus amyloliquefaciens (KJ767387.1), Bacillus velezensis (CP024897.1), Bacillus subtilis (KX827606.1), Serratia odorifera (NR_037110.1) and Exiguobacterium sibirricum (CP001022.1). The contact angle measurements of bacterial cells showed that all the strains had a hydrophilic character with ΔGiwi ranging from 17.02 to 32.80 mJ m2, strong electron donating (γ) and weak electron accepting (γ+) potentials. For substrates surfaces, we found that both sea-immersed stainless steels were hydrophilic and presented strong electron-donor and weak electron-acceptor characters. The analysis of images obtained by environmental scanning electron microscopy showed that bacterial cells were able to adhere on both sea-immersed stainless steels. 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