Asian Journal of Scientific Research1992-14542077-2076Asian Network for Scientific Information10.3923/ajsr.2018.401.408Pseudomonas aeruginosa in Iraq]]>AljanabyAhmed Abduljabbar Jaloob32018113Background and Objective:Pseudomonas aeruginosa (P. aeruginosa) is considered one of the most dangerous opportunistic bacteria worldwide, live in hospitals and other environments such as sewage water and soil and cause different infections include pneumonia. The main aim of this study was to investigate antibiotics susceptibility pattern and genotypic detection of virulence genes in P. aeruginosa isolates from clinical and environment sources. Materials and Methods: A total of 120 P. aeruginosa isolates were collected from two sources, 60 isolates from sputum of patients with pneumonia and 60 isolates from sewage water. Antibiotics susceptibility testing was done according to Kirby-Bauer method. Polymerase chain reaction technique was performed to detect the prevalence of six virulence-associated genes. Results: All 120 isolates (100%) were resistance to amoxicillin. While, imipenem was the best efficient antibiotic against clinical and sewage water isolates. Fifty five clinical isolates (91.6%) and 41 sewage water isolates (68.3%) were multi-drug resistance (MDR). The Pap A, Fim H, feoB, iutA, hly and kpsMTII genes prevalence in clinical isolates with percentages 91.6, 100, 80, 95, 20 and 100%, respectively, while the same genes prevalence in sewage water isolates with percentages, 51.6, 68.3, 41.6, 35, 3.3, 5 and 41.6%, respectively. Conclusion: This study suggested that there was positive correlation between the high prevalence of virulence-associated genes and increase of antibiotics resistance in P. aeruginosa isolates.]]>Biswas, J.K., M. Mondal, J. Rinklebe, S.K. Sarkar and P. Chaudhuri et al.,2017Pseudomonas aeruginosa and its synergistic benefits.]]>Environ. Geochem. Health,3915831593Koberg, S., E. Brinks, G. Fiedler, C. Husing and G.S. Cho et al.,2017Klebsiella pneumoniae bacteriophage PMBT1 isolated from raw sewage.]]>Genome Announcements, Vol. 5, No. 8.2017Laudy, A.E., P. Rog, K. Smolinska-Krol, M. Cmiel and A. Sloczynska et al.,2017Pseudomonas aeruginosa isolates in Warsaw, Poland, detected by various phenotypic and genotypic methods.]]>PLoS ONE, Vol. 12.2017Da Silva Junior, V.V., L.D. Ferreira, L.R. Alves, A.B. Cabral and P.R.L. de Araújo Jacome et al.,2017Pseudomonas aeruginosa harboring blaGES-1 and blaGES-11 in Recife, Brazil.]]>Rev. Soc. Bras. Med. Trop.,50764768Mao, J., A. Zaborin, V. Poroyko, D. Goldfeld and N.A. Lynd et al.,2017De novo synthesis of phosphorylated triblock copolymers with pathogen virulence-suppressing properties that prevent infection-related mortality.]]>ACS Biomater. Sci. Eng.,320762085Maura, D., A. Bandyopadhaya and L.G. Rahme,2018Pseudomonas aeruginosa quorum sensing studies.]]>Methods Mol. Biol.,1673227241El Fertas-Aissani, R., Y. Messai, S. Alouache and R. Bakour,2013Klebsiella pneumoniae strains isolated from different clinical specimens.]]>Pathol. Biol.,61209216The, H.C., A. Karkey, D.P. Thanh, C.J. Boinett and A.K. Cain et al.,2015Klebsiella pneumoniae.]]>EMBO Mol. Med.,7227239Labudda, L., D. Strapagiel, J. Karczewska-Golec and P. Golec,2017Klebsiella pneumoniae phages isolated from sewage in Poland.]]>Genome Announcements, Vol. 5, No. 45.2017Vuotto, C., F. Longo, M.P. Balice, G. Donelli and P.E. Varaldo,2014Klebsiella pneumoniae.]]>Pathogens,3743758Aljanaby, A.A.J. and H.M.R.J. Alhasnawi,2017Klebsiella pneumoniae isolated from different clinical sources in Al-Najaf province-Iraq.]]>Pak. J. Biol. Sci.,20217232Gharrah, M.M., A.M. El-Mahdy and R.F. Barwa,2017Klebsiella pneumoniae compared to nonproducing isolates.]]>Interdisciplin. Perspect. Infect. Dis., Vol. 2017.2017Aljanaby, A.A.J. and A.R. Medhat,2017Salmonella typhi isolated from patients infected with typhoid fever.]]>J. Biol. Sci.,17171184Dobrindt, U.,2005Escherichia coli.]]>Int. J. Med. Microbiol.,295357371Wiskur, B.J., J.J. Hunt and M.C. Callegan,2008Klebsiella pneumoniae endophthalmitis.]]>Invest. Ophthalmol. Visual Sci.,4949314938De Oliveira, F.P., B.M.F.B. Pires, K.D.C.F. de Almeida Silva, B.T.F. de Carvalho, L.A. Teixeira, G.R. de Paula and B.G.R.B. de Oliveira,2017Pseudomonas aeruginosa in chronic wounds.]]>J. Wound Ostomy Continence Nurs.,44528535Harnisz, M. and E. Korzeniewska,2018Aeromonas spp. in the municipal wastewater system and their dissemination in the environment.]]>Sci. Total Environ.,626377383Feglo, P.K. and M. Sewurah,2018Vibrio cholerae isolated from a large cholera outbreak in Ghana.]]>BMC Res. Notes, Vol. 11.2018Mac Faddin, J.F.,2000Biochemical Tests for Identification of Medical Bacteria.3rd Edn.,Pages: 912Pages: 912Baccer, R.W., M.D.K. Kirb, J.C. Sherris and M. Turek,1966Am. J. Clin. Pathol.,45493496CLSI.,2016Performance standards for antimicrobial susceptibility testing.2016Moulin-Schouleur, M., M. Reperant, S. Laurent, A. Bree and S. Mignon-Grasteau et al.,2007Escherichia coli strains of avian and human origin: Link between phylogenetic relationships and common virulence patterns.]]>J. Clin. Microbiol.,4533663376Yun, K.W., H.Y. Kim, H.K. Park, W. Kim and I.S. Lim,2014Escherichia coli of urinary tract infections and asymptomatic bacteriuria in children.]]>J. Microbiol. Immunol. Infect.,47455461El Zowalaty, M.E., A.A. Al Thani, T.J. Webster, A.E. El Zowalaty and H.P. Schweizer et al.,2015Pseudomonas aeruginosa: Arsenal of resistance mechanisms, decades of changing resistance profiles and future antimicrobial therapies.]]>Future Microbiol.,1016831706Aljanaby, A.A.J. and I.A.J. Aljanaby,2017Res. J. Pharm. Technol.,1032643270Solomon, F.B., F. Wadilo, E.G. Tufa and M. Mitiku,2017Pseudomonas aeruginosa and Acinetobacter baumanii in restricted settings of a referral hospital: A neglected condition.]]>Antimicrob. Resistance Infect. Control, Vol. 6.2017Gorny, R.L.,2004Ann. Agric. Environ. Med.,11185197Park, J., J.S. Kim, S. Kim, E. Shin and K.H. Oh et al.,2018Escherichia coli infections associated with drinking water at a school camp.]]>Int. J. Infect. Dis.,664550Gad, G.F., R.A. El-Domany, S. Zaki and H.M. Ashour,2007Pseudomonas aeruginosa isolated from clinical and environmental samples in Minia, Egypt: Prevalence, antibiogram and resistance mechanisms.]]>J. Antimicrob. Chemother.,6010101017Vahdani, M., L. Azimi, B. Asghari, F. Bazmi and A.R. Lari,2012Pseudomonas aeruginosa from infected burns.]]>Ann. Burns Fire Disasters,257881Xavier, D.E., R.C. Picao, R. Girardello, L.C. Fehlberg and A.C. Gales,2010Pseudomonas aeruginosa causing bloodstream infections in Brazil.]]>BMC Microbiol., Vol. 10.2010Chen, Z., H. Niu, G. Chen, M. Li, M. Li and Y. Zhou,2015Pseudomonas aeruginosa isolates from different wards in a Chinese teaching hospital.]]>Int. J. Clin. Exp. Med.,81940019405Walsh, T.R., M.A. Toleman, L. Poirel and P. Nordmann,2005Clin. Microbiol. Rev.,18306325Poole, K.,2007Ann. Med.,39162176Otton, L.M., M. da Silva Campos, K.L. Meneghetti and G. Corcao,2017Pseudomonas strains.]]>Arch. Microbiol.,199677682Karbasizade, V., P. Dehghan, M.M. Sichani, K. Shahanipoor, S. Sepahvand, R. Jafari and R. Yousefian,2017Pseudomonas aeruginosa.]]>Pak. J. Pharmaceut. Sci.,30585589Hoiby, N., T. Bjarnsholt, M. Givskov, S. Molin and O. Ciofu,2010Int. J. Antimicrob. Agents,35322332Murphy, K., A.J. Park, Y. Hao, D. Brewer, J.S. Lam and C.M. Khursigara,2014Pseudomonas aeruginosa PAO1.]]>J. Bacteriol.,19613061317Rivero, A., E. Gomez, D. Alland, D.B. Huang and T. Chiang,2010Klebsiella pneumoniae causing a liver abscess associated with infective endocarditis.]]>J. Clin. Microbiol.,48639641Victor, L.Y., D.S. Hansen, W.C. Ko, A. Sagnimeni and K.P. Klugman et al.,2007Klebsiella and clinical manifestations of K. pneumoniae bloodstream infections.]]>Emerg. Infect. Dis.,13986993Koczura, R. and A. Kaznowski,2003Yersinia high-pathogenicity island and iron uptake systems in clinical isolates of Klebsiella pneumoniae.]]>Microb. Pathog.,35197202May, T. and S. Okabe,2011Escherichia coli.]]>Environ. Microbiol.,1331493162Stehling, E.G., D.S. Leite and W.D. Silveira,2010Pseudomonas aeruginosa isolated from cystic fibrosis patients in Brazil.]]>Braz. J. Infect. Dis.,14462467Gutu, A.D., N.S. Rodgers, J. Park and S.M. Moskowitz,2015Pseudomonas aeruginosa high-level resistance to polymyxins and other antimicrobial peptides requires cprA, a gene that is disrupted in the PAO1 strain.]]>Antimicrob. Agents Chemother.,5953775387Tong, T.T., M. Morgelin, A. Forsgren and K. Riesbeck,2007Haemophilus influenzae survival during complement-mediated attacks is promoted by Moraxella catarrhalis outer membrane vesicles.]]>J. Infect. Dis.,19516611670Manning, A.J. and M.J. Kuehn,2011BMC Microbiol., Vol. 11.2011