Asian Journal of Scientific Research1992-14542077-2076Asian Network for Scientific Information10.3923/ajsr.2010.51.61Citrus paradisi Seed Extract on Doxorubicin-Induced
Testicular Oxidative Stress and Impaired Spermatogenesis]]>SaaluL.C. OsinubiA.A. JewoP.I. OyewopoA.O. AjayiG.O. 1201031The therapeutic value of Doxorubicin (DOX) as anticancer antibiotic is limited by its organotoxicity. It has been shown that free radicals are involved in doxorubicin-induced toxicity. Doxorubicin causes the generation of free radicals and the induction of oxidative stress, associated with cellular injury. Because of the great importance of DOX in cancer therapy, researchers have expended great efforts trying to prevent or attenuate the side effects of DOX administration. There has, however, been only a little success in this regard. In this study the ameliorating role of antioxidant-rich ethanolic seed extract of Citrus paradisi (CP) on DOX-induced testicular oxidative stress and impaired sperm parameters was investigated. Three experimental groups of Wistar rats were used; CP-alone group that received orally CP 10 mg kg-1 b.wt. daily for 14 days followed by intraperitoneal (i.p.) Normal Saline (NS) 2.5 mg kg-1 b.wt. DOX-alone group that had ip DOX 10 mg kg-1 b.wt. as a single dose. CP plus DOX-group that were similarly given DOX, but also had oral CP 10 mg kg-1 b.wt. pretreatment for 14 days. There was also a corresponding control group. The animals were autopsied 8 weeks after DOX or NS injections. Results showed that DOX-induced reduction in sperm motility and epididymal sperm concentrations as well as increase in total abnormal sperm rates were all normalized in the group pretreated with CP. Pretreatment with CP ameliorated the testicular content of Glutathione (GSH) and Auperoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) activities. Similarly, CP treatment attenuated the DOX-induced increase in testicular lipid peroxidation reflected by malondialdehyde (MDA) levels. These data indicate that CP protects the rat testis against DOX-induced oxidative stress and deranged sperm characteristics.]]>Aebi, H.E.,19831983pp: 273-286pp: 273-286WMA and APS,2002283R281R283Armando, C., S. Maythe and N.P. Beatriz, 199777463467Armstrong, L.L. and R.J. Lipsy,1993Atessahin, A., I. Karahan, G. Turk, S. Gur, S. Yilmaz and A.O. Ceribasi,2006214247Banerjee, H.N. and M. Verma, 20087149154Buege, J.A. and S.D. Aust,19781978pp: 302-310pp: 302-310Berthiaume, J.M. and K.B. Wallace,2007231525Bonadonna, G., M. Zambetti and P. Valagussa,1996273542547CCAC.,1985Vol. 23,pp: 45-47pp: 45-47Chularojmontri, L., S.K. Wattanapitayakul, A. Herunsalee, S. Charuchongkolwongse, N. Niumsakul and S. Srichairat,2005Phyllanthus urinaria L. on doxorubicin-induced cardiotoxicity.]]>2811651171Davies, K.J. and J.H. Doroshow,198626130603067Dowling, C. F. and J.F. Morton,19872nd Edn.,pp: 18-28pp: 18-28Duncan, D.B.,195713164176Ellman, G.L.,1959827077Endo, F., F. Manabe, H. Takeshima and H. Akaza,2003107277Frederick, C.A., L.D. Williams, G. Ughetto, G.A. van der Marel, J.H. van Boom, A. Rich and A.H. Wang,19902925382549Halliwell, B.,2008in vivo studies?]]>476107112Heggers, J.P., J. Cottingham, J. Gusman, L. Reagor and L. McCoy et al.,2002in vitro toxicity.]]>8333340Heller, C.G. and Y. Clermont, 1964140184186Jain, K.K.,20054311313Jegou, B., C. Pineau and J. Toppari,2002in vitro in Mammals.]]>2002pp: 3-60pp: 3-60Kalender, Y., M. Yel and S. Kalender,20052093945Kato, M., S. Makino, H. Kimura and T. Ota,2001265159Mornparler, R.L., M. Karon, S.E. Siegel and F. Avila, 19763628912895Ng, R., N. Better and M.D. Green,200633214Pacher, P., J.S. Beckman and L. Liaudet,200787315424Pigram, W.J., W. Fuller and L.D. Hamilton, 19722351719Prahalathan, C., E. Selvekuma and P. Varalakshmi,200520111116Priestman, T., 2008pp:130-136pp:130-136Quiles, J.L., J.R. Huertas, M. Battino, J. Mataix and C.M. Ramirez-Tortosa,20021807995Rotruck, J.T., A.L. Pope, H.E. Ganther, A.B. Swanson, D.G. Hafeman and W.G. Hoekstra,1973179588590Rukmini, M.S., B. D'Souza and V. D'Souza,200419114118Saalu, L.C., L.A. Enye and A.A. Osinubi,20091370374Saalu, L.C., G.O. Ajayi, A.A. Adeneye, I.O. Imosemi and A.A. Osinubi,2009Citrus paradisi Macfad) as an effective attenuator of doxorubicin-induced oxidative stress in the rat heart.]]>54452Sachs, A.,1997pp: 775-795pp: 775-795Sikka, S.C., 199617886Singh, K.K., 20054583584Snedecor, G.W. and W.G. Cochran,19807th Edn.,pp: 507pp: 507Sonmez, M., G. Turk and A. Yuce,20056320632072Suominen J.S., J. Linderborg, J. Nikula, H. Hakovirta, H. Parvinen and J. Toppari, 2003N-ethyl-nitrosouria on stage-specific apoptosis and DNA synthesis in the mouse spermatogenesis.]]>143163173Ursini, F., F. Tubaro, J. Rong and A. Sevanian,199957241249Winterbourn, C.C., R.E. Hawkins, M. Brian and R.W. Carrell,197585337341Wolinsky, J.B. and M.W. Grinstaff,20086010371055Yokoi, K. and Z.K. Mayi,20042907885Yeh, Y.C., L. Hui-Chi and T. Chih-Tai, 200777217217Bromberg, L.,200812899112