Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2021.470.476Leucas calostachys Root Extracts]]>K. KimutaiNicholas OgutuPhilip A. MutaiCharles 32021203Background and Objective: Traditional healers have long used plants to treat bacterial infections caused by resistant bacteria. Hence, there is an increased interest in the ethnopharmacological approach to identify new novel compounds from plants to treat these infections. The objective of this study, therefore, was to test successive extracts and their fractions. Materials and Methods: The roots of Leucas calostachys were obtained, dried and ground. The total methanol extract was obtained and screen for antibacterial activities. Successive extracts were extracted using four different solvents hexane, dichloromethane, ethyl acetate and methanol. The bioactive solvent extracts were fractionated. Successive extracts and fractions were then screened for antibacterial activities against ten pathogenic bacteria in vitro using the disk diffusion method. Results: The results indicated the solvent extracts and fractions were active against both gram-negative and gram-positive bacteria with the lowest MIC value of 3.15 mg mL1 obtained from total methanol extract against methicillin-resistant Staphylococcus aureus. Conclusion: This demonstrated that hexane extracts and fractions could be helpful in the management of resistant bacterial infections. This work is the first attempt to fractionate and test fractions of L. calostachys, which can be used for the development of phytomedicine.]]>Gaoue, O.G., M.A. Coe, M. Bond, G. Hart, B.C. Seyler and H. McMillen,201771269287Marasini, B.P., P. Baral, P. Aryal, K.R. Ghimire and S. Neupane et al.,20152015Nicholas, K., C. Mutai, E.W. Njenga, P. Jeruto, L. Ng'eny and R. Korir,201658896Kipkore, W., B. Wanjohi, H. Rono and G. Kigen,20142014Nicholas, K.,2017In vitro cytotoxicity of three selected plant extracts from Kenya. ]]>6144152Gori, A., L.B. Nascimento, F. Ferrini, M. Centritto and C. Brunetti,20202020Hossan, M.S., H. Jindal, S. Maisha, C. Samudi Raju and S.D. Sekaran et al.,20185201208Ochwang’I, D.O., C.N. Kimwele, J.A. Oduma, P.K. Gathumbi, S.G. Kiama and T. Efferth,20162016Kigen, G., F. Some, J. Kibosia, H. Rono and E. Kiprop et al.,20142014Schultz, F., G. Anywar, C.L. Quave and L.A. Garbe,20212021Ngemenya, M.N., G. Djeukem, K.D. Nyongbela, P. Bate, S.B. Babiaka, E. Monya and R.K. Kanso,20192019Koehn, F.E. and G.T. Carter,20054206220Motamedi, H., E. Darabpour, M. Gholipour and S.M.S. Nejad,2010Plantago ovata and Oliveria decumbens endemic in iran against some pathogenic bacteria.]]>6117122Larson, E.C., C.D. Pond, P.P. Rai, T.K. Matainaho, P. Piskaut, M.R. Franklin and L.R. Barrows,20162016Saavedra, M.J., A. Borges, C. Dias, A. Aires, R.N. Bennett, E.S. Rosa and M. Simões,20106174183Ocampo, P.S., V. Lázár, B. Papp, M. Arnoldini and P.A. zur Wiesch et al.,20145845734582Korir, R.K., C. Mutai, C. Kiiyukia and C. Bii,20126370382Shulman, S.T., A.L. Bisno, H.W. Clegg, M.A. Gerber and E.L. Kaplan et al.,20125586102Farman, M., M. Yasir, R.R. Al-Hindi, S.A. Farraj, A.A. Jiman-Fatani, M. Alawi and E.I. Azhar,20192019Ali, S., M.R. Khan, Irfanullah, M. Sajid and Z. Zahra,2018Parrotiopsis jacquemontiana (Decne) Rehder.]]>2018Sülsen, V.P., E. Lizarraga, N.Z. Mamadalieva and J.H.G. Lago,20172017