Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2020.185.190Spondias pinnata and its Secondary Metabolite]]>LalloSubehan Hasma Sartini AswadMuhammad 22020192Background and Objective: Ambarella (Spondias pinnata (L.f.) Kurz), Family Anacardiaceae has been traditionally used as an antibacterial in Indonesia. Only the apical leafs of this plant traditionally used for treatment of bacterial infection. The present study aimed to investigate the effect of the leaf position on antibacterial activity and constituent profile of the extracts. Materials and Methods: The leaf was collected in three positions (apical, middle and basal leaf) and macerated with 70% ethanol. Antibacterial activity was tested by agar diffusion method using Muller Hinton Agar (MHA) medium against Staphylococcus aureus and Pseudomonas aeruginosa with amoxicillin as positive control. Characterization of its chemical constituent was performed with chromatography method. Results: The results showed that ethanol extract of ambarella leaf contain group compounds of flavonoids, alkaloids, tannins and saponins. The highest antibacterial activity against S. aureus and P. aeruginosa was observed by the apical position of the leaf with inhibition zones of 14.30 and 14.43 mm, respectively. HPLC chromatogram profile of apical leaf extracts shows different pattern with middle and basal leaf. Fractionation of the active extract given n-hexane and ethyl acetate fractions with inhibition zones of 13.27 and 12.91 mm and ethyl acetate fractions of 12.49 and 12.41 mm against S. aureus and P. aeruginosa, respectively. While no inhibitory activity was observed on chloroform and water fractions. Conclusion: Each position of ambarella leaf has different constituent character and among them, the apical leaves have a stronger inhibitory activity than the other position.]]>Cowan, M.M.,199912564582Lewis, K.,201312371387Das, J., A. Mannan, M.M. Rahman, M.A. Dinar and M.E. Uddin et al.,2011Spondias pinnata and its different pharmacological activity like-antioxidant, cytotoxic, antibacterial potential and phytochemical screening through in-vitro method.]]>218061812Khare, C.P.,20071st Edn., Pages: 900Pages: 900Muhammad, A., M. Rahman, A.N.M.H. Kabir, S. Kabir and M. Hossain,2011Spondias pinnata (Linn. f.) Kurz fruit extract.]]>2265267Arif, M., K. Zaman, S. Fareed and M. Hussain,2008Spondias mangifera bark.]]>1177182Acharyya, S., G.K. Dash, S. Mondal and S.K. Dash,2010Spondias mangifera Willd root.]]>2184190Hazra, B., S. Biswas and N. Mandal,2008Spondias pinnata.]]>2008Panda, S.K., N. Patra, G. Sahoo, A.K. Bastia and S.K. Dutta,20122123134Jain, P., K.R. Hossain, T.R. Mishu and H.M. Reza,2014Spondias pinnata Kurz. leaves.]]>4183195Bora, N.S., B.B. Kakoti, B. Gogoi and A.K. Goswami,2014Spondias pinnata: A review.]]>511381145Setyorini, H.A., A.A. Kurniatri, R. Adelina and Winarsih,2016Annona muricata L.) from three growing places.]]>44279286Hariyani, H., E. Widaryanto and N. Herlina,2015Pogostemon cablin Benth.).]]>3205211Permata, D.A. and A. Asben,2017217985Harborne, J.B.,1973Pages: 278Pages: 278Biemer, J.J.,19733135140Sai, K., R. Thapa, H.P. Devkota and K.R. Joshi,20192019Hua-Bin, Z., D. Ai-Qin, Z. Xin-Ling, W. Pei-Hai, L. Wei-Jie, Y. Guo-Sheng and H.Y. Aboul-Enein,20122012Fan, X.H., Y.Y. Cheng, Z.L. Ye, R.C. Lin and Z.Z. Qian,2006555217224Da Costa Cordeiro, B.M.P., N.D. de Lima Santos, M.R.A. Ferreira, L.L.C. de Araújo and A.R. Carvalho Junior et al.,2018Spondias tuberosa (Anacardiaceae) leaves has antioxidant activity and is an anti-Candida agent by causing mitochondrial and lysosomal damages.]]>2018Lee, L.S., S.H. Kim, Y.B. Kim and Y.C. Kim,20141991739186Subehan, Y. Rifai and Mufidah,2013Caesalpinia sappan L.) extracts.]]>50713Da Silva, A.R.A., S.M. de Morais, M.M.M. Marques, D.F. de Oliveira and C.C. Barros et al.,2012Spondias species from Northeastern Brazil.]]>50740746