Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2020.152.165Lathyrus Species using RAPD, ISSR and SCoT Markers]]>A. OsmanSamira B.M. AliHoda 22020192Background and Objective:Lathyrus (Leguminosae, Papilionoideae) is a member of Viciae tribe, which consists of about 150~160 annual and perennial species, it has an importance as foodstuffs worldwide. The main goal of this study was to determine the genetic diversity of 5 Lathyrus species (L. articulates L., L. hierosolymitanus Boiss, L. latifolius L. (France), L. pseudocicera pamp (Israel) and L. tuberosus L. (Soviet union)) by using different molecular markers as Randomly Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeats (ISSR) and Start Codon Targeted (SCoT). Materials and Methods: The DNA of the 5 Lathyrus species was used to amplify different fragments via Polymerase Chain Reaction (PCR) by using 6 RAPD, 8 ISSR and 8 SCoT primers. Results: The ISSR markers gave a maximum values of polymorphism (96.81%), Polymorphic Information Content (PIC) (0.305) and gene diversity (0.387) while the minimum values of polymorphism (94.2%), PIC (0.301) and gene diversity (0.379) documented in RAPD markers. The SCoT markers gave a high values of polymorphism (96%), PIC (0.302) and gene diversity (0.381) but slightly less than ISSR values. Also Unweighed Pair Group Arithmetic (UPGMA) results of collective data nearly similar to the UPGMA of ISSR marker. These results indicated that ISSR and SCoT markers considered a powerful marker for discrimination and identification of 5 studied Lathyrus species. Conclusion: The phylogenetic relationship among the studied species in descending order is L. tuberosus is more related to L. hierosolymitanus then comes L. latifolius and then L. articulates, whereas L. pseudocicera at a distant position from the other species but still related to L. hierosolymitanus.]]>Young, N.D., J. 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