Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2013.61.70Sorghum bicolor ssp. bicolor
(L.) Moench using ISSR Markers]]>TadesseHailekiros FeyissaTileye 22013122Ethiopia is one of the origins and centers of genetic diversity
of sorghum. As sorghum ranks fifth in global cereal production, the study of
its genetic diversity from its center of origin is important for its improvement.
Therefore, the aim of the present study was to evaluate the within and among
population genetic diversity of sorghum from Ethiopia using Inter Simple Sequence
Repeat markers. Eight individuals from each of eleven sorghum populations of
highland, intermediate and lowland ecological zones and one population of improved
variety and stay-green cultivars were used for the study. DNA was extracted
from young leaves and PCR was conducted using seven primers. A total of 55 clear
and reproducible bands with 100% polymorphism were generated. The total genetic
diversity (GD) and Shannons diversity index (I) were 0.205 and 0.296,
respectively. North Gondar Intermediate altitude population showed the highest
gene diversity (0.3) while the lowest diversity (0.1) was exhibited by North
Shewa population. AMOVA showed that 44.45, 30.84 and 24.71% of the total variation
was attributed to within populations, among populations and among groups, respectively.
Group based UPGMA exhibited four distinct clusters whereas the population based
UPGMA showed all populations clustered within its own ecological zone except
North Gondar (I1) intermediate altitude population and improved varieties
and stay-green (IM) individuals that were clustered in the highland populations.
Intermediate altitude populations exhibited higher genetic diversity than low
and high altitude populations indicating these high and low altitude populations
may have originated from the intermediates through ecological adaptations.]]>Abdi, A., E. Bekele, Z. Asfaw and A. Teshome,2002Sorghum bicolor (L.) Moench) landraces in qualitative characters in North Shewa and South Welo, Ethiopia.]]>137161172Ayana, A. and E. Bekele,2000Sorghum bicolor (L.) Moench) germplasm from Ethiopia and Eritrea: Quantitative characters.]]>11591104Dje, Y., M. Heuretz, C. Lefebvre and X. Vekemans,2000100918925Ejeta, G., B. Goldsbrough, R. Tuinstra, E. Grote and A. Menkir et al.,20002000pp: 81-89pp: 81-89Engels, J.M.M. and J.G. Hawkes,19911991pp: 23-26pp: 23-26Iqbal, A., B. Sadia, A.I. Khan, F.S. Awan, R.A. Kainth and H.A. Sadaqat,2010Sorghum bicolor L. Moench) germplasm of Pakistan.]]>9756764Price, H.J., S.L. Dillon, G. Hodnett, W.L. Rooney, L. Ross and J.S. Johnston,200595219227Kudadjie, C.,20062006Leder, I.,20042004pp: 570-578pp: 570-578McGuire, S.,20002000pp: 338-448pp: 338-448Mekbib, F., A. Bjornstad, L. Sperling and G. Synnevag,2009Sorghum bicolor (L.) Moench] in the centre of diversity, Ethiopia.]]>14559Nguni, D., M. Geleta and T. Bryngelsson,2011Sorghum bicolor (L.) Moench) accessions of Zambia as revealed by Simple Sequence Repeats (SSR).]]>1485262Ohsawa, T. and Y. Ide,200817152163Prakash, S.P.J., K.R. Biji, S.M. Gomez, K.G. Murthy and R.C. Babu,2006Sorghum bicolour L. Moench) accessions using RAPD markers.]]>1109112Teshome, A., D. Patterson, Z. Asfew, K. Torrance and T. Arnason,2007Sorghum bicolor landrace diversity and farmers selection criteria over space and time, Ethiopia.]]>5412191233Tesso, T., I. Kapran, C. Grenier, A. Snow and P. Sweeney et al.,20084814251431Tsehaye, Y., Z. Abera, A. Kebede and B. Ghebremichael,2009Sorghum bicolor (L.) Moench) diversity management in situ and livelihood resilience in south and central Tigray region.]]>16794Wang, M., B.J. Oppedijk, X. Lu, B. van Duijn and R.A. Schilperoort,19963211251134Fang, X.E., Q. Chen, L.P. Yin and W. Wang,20083414801483Zheng, L.Y., X.S. Guo, B. He, L.J. Sun and Y. Peng et al.,2011Sorghum bicolor).]]>2011