Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2013.46.50Daucus carota L.]]>NaganandaG.S. RajathS. AgarwalDisha MathewK. Ruby RajanS. Sundara 12013121Daucus carota L. is an important vegetable food crop with high nutritive value. The changing climate, pollution, loss of vegetation has affected the crop quality and there is a need to improve the variety. So the present investigation was taken with an aim to induce mutation in callus cell lines for crop improvement. Callus induction from stem and leaf explants of in vitro grown Daucus carota L. plantlets was observed on 1X MS medium with 3% sucrose , 1% agar fortified with 2 mg L-1 2,4-D. Mutation was induced in Daucus carota L callus cell lines by sodium azide and UV radiations. Callus cell lines grown on media containing 200 μM of sodium azide gave the best result with callus multiplication. Callus exposed at different time intervals under UV radiation showed multiplication of callus in all the exposures. Shoot induction was achieved for both the mutants on 2 mg L-1 BAP containing medium. The physiological estimation revealed that protein content (0.460±0.04 mg mL-1) in 6 min UV exposed cell lines and carbohydrate content (0.820±0.08 mg mL-1) in 4 min UV exposed cell lines are high when compared to control cell lines, sodium azide and other UV mutated cell lines. Mutation was confirmed through DNA fingerprinting.]]>Ehsanpour, A.A. and R. Razavizadeh,2005Medicago sativa) callus.]]>812211224Akhaury, S.B., A.N. Sinha and A.K. Sinha,1996Hordeum vulgare (L.) Karan 16.]]>415Arulbalachandran, D., L. Mullainathan and S. Velu,2009Vigna mungo (L.) Hepper) with effect of DES and COH in M2 generation.]]>1213218Al-Gawwad, A. and A.H. Makka,2009Mirabilis jalapa.]]>2009Pant, B. and S. Manandhar,2007In vitro propagation of carrot (Daucus carota) L.]]>55153Bertagne-Sagnard, B., G. Fouilloux and Y. Chupeau,1996Linum usitatissimum).]]>47189194Broertjes, C. and A.M. van Harten,1988Pages: 345Pages: 345Khan, S., M.R Wani, M. Bhat and K. Parveen,20041167Khan, S., M.R. Wani and K. Parveen,200629143145Kolb, C.A., M.A. Kaser, J. Kopecky, G. Zota, M. Riedere and E.E. Pfundel,2001127863875Doyle, J.J.,1990121315Mostafa, G.G.,2011Helianthus annuus L.]]>57685Hedge, J.E. and B.T. Hofreiter,19621962pp: 1-19pp: 1-19Mensah, J.K. and B. Obadoni,2007Arachis hypogaea L.).]]>6668671Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Kulthe, M.P. and V.S. Kothekar,2011Cicer arietinum L.).]]>33942Murashige, T. and F. Skoog,196215473497Ojomo, A.O., O. Omueti, J.A. Raji and O. Omueti,1979216164Piccioni, E., G. Barcaccia, M. Falcinelli and A. Standardi,1997158556562Sinha, A. and J.P. Lal,20071 parameters and qualitative changes induced in M2 generation in lentil.]]>30180185Bulk., R.W., H.J.M. Loffler, W.H. Lindhout and M. Koornneef,199080817825Tavares, A.C., L.R. Salgueiro and J.M. Canhoto,2010In vitro propagation of the wild carrot Daucus carota L. subsp. halophilus (Brot.) A. Pujadas for conservation purposes.]]>464756Wongpiyasatid, A., S. Chotechuen, P. Hormchan, S. Ngampongsai and W. Promcham,200034443449Yau, Y.Y., S.J. Davis, A. Ipek and P.W. Simon,2008Daucus carota L.) callus.]]>7664671