Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2012.294.299Stemona curtisii Hook.f.]]>PaleeJiraporn DheeranupattanaSrisulak JatisatienrAraya WangkarnSunanta MungkornasawakulPitchaya PyneStephan UngAlison SastrarujiThanapat 62012116The aim of this experiment was to study the optimal age of
Stemona curtisii plantlets for acclimatization. The in vitro shoots
of S. curtisii were cultured on Murashige and Skoog solid medium supplemented
with 1 mg L-1 naphthalene acetic acid to induce roots. Then, the
plantlets from in vitro culture of 4, 8 and 12 week-old were transferred
into the soil and their survival rate during the acclimatization process in
the greenhouse was investigated. It was found that the 8 week-old plantlets
had the highest survival rate of 80%. However, to enhance the survival rate
of these plantlets, a soilless culture technique as a possible approach for
the acclimatization was considered. Eight week-old plantlets were transferred
into either, soil, a hydroponic system (nutrient film technique), coconut fiber
or sand. The plantlets which were cultivated in coconut fiber or hydroponic
culture showed 100% survival rate with the highest average number of new roots
per plant. However, the mean root length of the plantlets grown in hydroponic
system was significantly higher than that grown in coconut fiber.]]>Albaho, M., N. Bhat, H. Abo-Rezq and B. Thomas,2009Capsicum annuum.]]>28227233Donnelly, D.J. and W.E. Vidaver,1984109172176Gilly, C., R. Rohr and A. Chamel,1997Hedera helix L.) plants In vitro and during Ex vitro acclimatization.]]>80139145Grantz, D.A., 199013667679Hassanpanah, D. and M. Khodadadi, 2009In vivo condition.]]>9243248Hazarika, B.N.,2006In vitro culture of plants.]]>108105120Kozai, T., K. Iwabuchi, K. Watanabe and I. Watanabe,1991In vitro and changes in nutrient composition of the medium.]]>25107115Kung'u, B.J., J. Kihara, D.N. Mugendi and H. Jaenicke,20083359364Kurtar, E.S., A. Balkaya and N.O. Okumus,2010In vitro plantlets of winter squash (Cucurbita maxima duch. ex lam.) and pumpkin (Cucurbita moschata duch. ex Poir.) in acclimatization.]]>1148154Lamhamedi, M.S., H. Chamberland and F.M. Tremblay,2003In vitro acclimatization.]]>118554561Lavanya, M., B. Venkateshwarlu and B.P. Devi,20098218222Lee, N., H.Y. Wetzstein and H.E. Sommer,1988In vitro and In vivo developed sweetgum leaves.]]>113167171Mungkornasawakul, P., S.G. Pyne, A. Jatisatienr, D. Supyen and C. Jatisatienr et al.,2004Stemona curtisii: Structure of a new pyrido [1,2-a] azepine Stemona alkaloid.]]>67675677Murashige, T. and F. Skoog,196215473497Nhut, D.T., N.T.D. Huong and D.V. Khiem,2004In vitro plantlets of taro (Colocasia esculenta spp.) using hydroponics.]]>101207212Padilla, I.M.G., K. Webb and R. Scorza,2003Prunus domestica L.).]]>223845 Posposilova, J., I. Ticha, P. Kadlecek, D. Haisel and S. Plzakova,1999ex vitro conditions.]]>42481497Preece, J.E. and E.G. Sutter,19911991pp: 71-93pp: 71-93Rodrigues, P.H.V., A.M.L.P. Lima, G.M.B Ambrosano and M.F.B. Dutra,2005Heliconia bihai (Heliconiaceae) plants.]]>62299301Sastraruji, T.,2006Stemona extract and its application in agricultural use.]]>2006Sciutti, R. and S. Morini,1993In vitro culture on some growth characteristics of a plum rootstock during shoot proliferation and rooting and on plantlet survival.]]>7153156Serret, M.D. and M.I. Trillas,2000Gardenia jasminoides Ellis leaflets cultured In vitro.]]>161281289Thomas, P.,1998345256Wardle, K., E.B. Dobbs and K.C. Short,1983in vitro acclimatization of aseptically cultured plantlets to humidity.]]>108386389Wetzstein, H.Y. and H.E. Sommer,1982Liquidambar styraciflua (Hamamelidaceae) during acclimatization.]]>6915791586Whish, J.P.M., R.R. Williams and A.M. Taji,1992In vitro.]]>319231236Zobayed, S.M.A., F. Afreen, C. Kubota and T. Kozai,2000Ipomoea batatas grown photoautotrophically under forced ventilation and photomixotrophically under natural ventilation.]]>86603610