Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2012.195.199Momordica cochinchinensis
Spreng. Seed and Its Effects to Spodoptera litura ]]>ArimatsuPanida SawangsookOrawan 42012114This research was conducted to determine the effect of trypsin inhibitor on growth and development of Spodoptera litura. Trypsin Inhibitor from Momordica cochinchinensis (MMCTI) seed was extracted and purified using Ion Exchange Chromatography with CM-52 column. Trypsin Inhibitor Unit (TIU) was identified by using BApNA as substrate. The MMCTI showed 0.34 specific inhibitory units and mass numbers was 3165.03. In larval feeding studies, MMCTI were found to retard growth and development of S. litura. Larvae fed on diet containing MMCTI showed reduction in weight that was dose-dependent. Larval mortality ranged from 8 to 15% of the larval population. Reduction in pupal weight by 25% and pupal mortality by 27% were also observed with 12 TIU g-1 diet. Malformed adults (5-16%) were recorded as an effect of abnormal development. Fertility was affected as the egg-laying incidence reduced from 810 egg/female (control) to 298 egg/female (12 TIU g-1 diet). This result indicated that MMCTI is an efficient inhibitor of proteinases in the S. litura larvae. Being small peptide, it would be easy to express MMCTI in plants to confer protection against devastating pests such as S. litura.]]>Atiwetin, P., S. Harada and K. Kamei,2006Benincasa hispida (Thunb) Cogn.) seeds.]]>70743745Bown, D.P., H.S. Wilkinson and J.A. Gatehouse,1997Helicoverpa armigera, are members of complex multigene families.]]>27625638Chuethong, J., K. Oda, H. Sakurai, I. Saiki and W. Leelamanit,2007Momordica cochinchinensis.]]>30428432Damle, M.S., A.P. Giri, M.N. Sainani and V.S. Gupta,2005Lycopersicon esculentum Mill, Cv. Ghanashree).]]>6626592667De Leo, F., M. Volpicella, F. Licciulli, S. Liuni, R. Gallerani and L.R. Ceci,200230347348De Shan, M., L.H. Hu and Z.L. Chen,2001Momordica cochinchinensis Spreng.]]>15139145Franco, O.L., R.C.D. Santos, J.A. Batista, A.C. Mendes and M.A.D. Araujo et al.,200363343349Giri, A.P., A.M. Harsulkar, M.S.B. Ku, V.S. Gupta, V.V. Deshpande, P.K. Ranjekar and V.R. Franceschi,2003Helicoverpa armigera gut proteinases from winged bean seeds.]]>63523532Huang, B., T.B. Ng, W.P. Fong, C.C. Wan and H.W. Yeung,1999Momordica cochinchinensis, the Chinese grug mubiezhi.]]>31707715Inanaga, H., D. Kobayasi, Y. Kouzuma, C. Aoki-yasunaga, K. Iiyama and M. Kimura,2001Spodoptera exigua larvae.]]>6522592264Lee, K.P.,2010Spodoptera litura (Fabricius).]]>5616851695Perry, L.M.,19801st Edn.,Pages: 632Pages: 632Putiyanan, S.,2004Roy, D.N. and S.P. Rao,1971Lathyrus sativus.]]>19257259Ryan, C.A.,199028425449Sharma, P., A.K. Nath, R. Kumari, S.V. Bhardwaj,2012Albizia lebbeck seeds.]]>23131137Slansky, F. Jr. and J.M. Scriber,19851985pp: 87-163pp: 87-163Telang, A., V. Booton, R.F. Chapman and D.E. Wheeler,20014710551064Telang, M., A. Srinivasan, A. Patankar, A. Harsulkar and V. Joshi et al.,2003Helicoverpa armigera and Spodoptera litura.]]>63643652Telang, M.A., P. Pyati, M. Sainani, V.S. Gupta and A.P. Giri,2009Helicoverpa armigera.]]>16371380Tamhane, V.A., A.P. Giri, M.N. Sainani and V.S. Gupta,2007Capsicum annuum adversely affect the growth and development of Helicoverpa armigera.]]>4032938Vuong, L.T., S.R. Dueker and S.P. Murphy,2002Momordica cochinchinensis (gac).]]>75872879Vuong, L.T. and J.C. King,200324224230Wong, R.C., W.P. Fong and T.B. Ng,2004Momordica cochinchinensis seeds, the Chinese drug mubiezhi.]]>25163169