Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2009.361.367RolA, B and C Genes of Agrobacterium rhizogenes in Nicotiana glauca and its Hybrid]]>SuneethaD.R.S. ArundhatiA. RaoG.S. JoshuaP.V. 5200985The present study tends to investigate the role of DNA methylation in silencing and expression of rolA, B and C genes in Nicotiana glauca and its hybrid, respectively. Exposure of the hybrid seed to 5-azacytidine, a DNA hypomethylating agent resulted severe alterations in morphology, early tumor induction and loss of phytohormone independent growth. On the contrary, DNA hypermethylation caused by cefotaxime treatment resulted in enhanced rate of tumorigenesis and callus growth. The above results convey that changes in DNA methylation in the hybrid plants possess a significant effect on tumorigenesis. Therefore, DNA isolated from the parents, hybrid and tumor were restriction digested with cytosine methylation specific isoschizomers MspI and HpaII; and Southern analysis was carried out using rolA, B and C genes as probes. The size of the bands differed in MspI and HpaII digests of N. glauca, hybrid and tumor whereas no signals were observed in Nicotiana langsdorffii. Results were discussed in terms of silencing of rolA, B and C genes in N. glauca and their expression in the hybrid. Hence, it could be concluded that DNA hypermethylation of rol genes in N. glauca resulted in their silencing causing normal morphology and behavior of the plant, whereas DNA hypomethylation of rol genes was responsible for their expression in the hybrid causing morphological abnormalities and tumorigensis.]]>Aoki, S.,2004Agrobacterium to Nicotiana.]]>117329337Bulgakov, V.P.,200826318324Casanova, E., M.I. Trillas, L. Moysset and A. Vainstein,2005rol genes in floriculture.]]>23339Chilton, M.D. and Q. Que,2003133956965Christensen, B., S. Sriskandarajah, M. Serek and R. Muller,2008Kalanchoe blossfeldiana with rol-genes is useful in molecular breeding towards compact growth.]]>2714851495Dellaporta, S.L., J. Wood and J.B. Hicks,198311921Ehrlich, M.,200631251274Estruch, J.J., J. Schell and A. Spena,1991rolB plant oncogene hydrolyses indole glucosides.]]>1031253128Estruch, J.J., D. Chriqui, K. Grossmann, J. Schell and A. Spena,1991rolC is responsible for the release of cytokinins from glucoside conjugates.]]>1028892895Furner, I.J., G.A. Huffman, R.M. Amasino, D.J. Garfinkel, M.P. Gordon and E.W. Nester,1986Agrobacterium transformation in the evolution of the genus Nicotiana.]]>319422427Jones, P.A. and P.W. Laird,199921163167Kovarik, A., H. Van Houdt, A. Holy and A. Depicker,20004674751Murashige, T. and F. Skoog,196215473497Popiela, A., G. Keith, A. Borzecki, G. Popiela, M. Manowiec and M. Gabrys,200425145149Schmitt, F., J.O. Edward and J. Jean,1997Nicotiana tabacum plants.]]>27215341540Shkryl, Y.N., G.N. Veremeichik, V.P. Bulgakov, G.K. Tchernoded and N.P. Mischenko et al.,2008rolA B and C genes on anthraquinone production in Rubia cordifolia transformed calli.]]>100118125Southern, E.M.,197598503517Sun, L.Y., M.O. Monneuse, J. Martin Tanguy and D. Tepfer,1991rolA locus from the Ri TL-DNA of Agrobacterium rhizogenes.]]>80145156Suzuki, K., I. Yamashita and N. Tanaka,2002Agrobacterium rhizogenes infection during their evolution.]]>32775787Udagawa, M., S. Aoki and K. Syono,20044510231031