Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2008.454.460Vahabi SedehiAbbas Ali SolookiMahmood ArzaniAhmad GhanbariAhmad LotfiAsefeh Ali ImamjomehAbbas BahramiShahram 5200875This study was carried out to evaluate morphological
traits of the grass pea landraces using a randomized complete block design
with 3 replications at Research Farm of Isfahan University of Technology
and evaluation genetic diversity of 20 grass pea landraces from various
locations in Iran were investigated using 32 RAPD and ISJ primers at Institute
of Agriculture of Biotechnology of University of Zabol. Analysis of variance
indicated a highly significant differences among 20 grass pea landrace
for the morphological traits. Average of polymorphism percentage of RAPD
primer was 73.9%. Among used primer, 12 random primers showed polymorphism
and a total of 56 different bands were observed in the genotypes. Jafar-Abad
and Sar-Chahan genotypes with similarity coefficient of 66% and Khoram-Abad
2 and Khoram-Abad 7 genotypes with similarity coefficient of 3% were the
most related and the most distinct genotypes, respectively. Fourteen primers
out of 17 semi random primers produced 70 polymorphic bands which included
56% of the total 126 produced bands. Genetic relatedness among population
was investigated using Jacard coefficient and Unweighted Pair Group Mean
Analysis (UPGMA) algorithm. The result of this research verified possibility
of use of RAPD and ISJ markers for estimation of genetic diversity, management
of genetic resources and determination of repetitive accessions in grass
pea.]]>Campbell, C.G.,1987Lathyrus germplasm LS8246.]]>27821821Campbell, C.G., R.B. Mehra, S.K. Agrawal, Y.Z. Chen and A.M. Abd El-Moneim et al.,1994Lathyrus sativus).]]>73167175Dellaporta, S.L., J. Wood and J.B. Hicks,198311921Devos, K.M. and M.D. Gale,199284567572Fahima, T., G.L. Sun, A. Beharav, T. Krugman, A. Beiles and E. Nevo,1999Triticum dicoccoides, in Israel.]]>98434447Fernandez, M.E., A.M. Figueiras and C. Benito,2002104845851Ghobrial, K.M., A.H.A. Younis and A.Z. Halim,1983613641Hossaert, M. and M. Valero,1986Lathyrus Species.]]>1986pp: 175-185pp: 175-185Jackson, M.T. and A.G. Yunus,1984Lathyrus sativus L.) and wild species.]]>33549559Karp, A., K. Edwards, M. Bruford, B. Vosman and M. Morgante et al.,199715625628Keatinge, J.D.H.B., A. Asghar, B.R. Khan, A.M. Abd El Moneim and S. Ahmad,19911664857Pandey, R.L., S.K. Agrawal, M.W. Chitale, R.N. Sharma, O.P. Kashyap, A.K. Geda, H.K. Chandrakar and K.K. Agrawal,1995L. sativus L.) germplasm.]]>1st Edn.,Pejic, I., P. Ajmone-Marsan, M. Morgante, V. Kozumplick, P. Castiglioni, G. Taramino and M. Motto,19989712481255Rafalski, A., L. Madej, I. Wisniewska and M. Gawelo,20027471475Rohlf, F.,1998Version 2.2,Weining, S. and R.J. Henry,1995Hordeum spontaneum) germplasm using the polymerase chain reaction.]]>42273281Yee, E., K., Kidwell, G.R. Sills and T.A. Lumpkin,1999Vigna angularis (Azuki) accessions on the basis of RAPD and AFLP markers.]]>39268275 Yunus, A.G. and M.T. Jackson, 1991Lathyrus sativus L.).]]>106319328Zoghlami, N. Chrita, I. Bouamama, B. Gargouri and H. Zemni,2007Opuntia ficus indica (L.) Mill.) in Tunisia. ]]>113134141