Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2008.327.330Guarianthe skinnerii Inoculated with Trichoderma harzianum]]>Gutiarrez-MiceliF.A. Ayora-TalaveraT. Abud-ArchilaM. Salvador-FigueroaM. Adriano-AnayaL. Arias HernandezM.L. DendoovenL. 3200873The objective of this study was to investigate survival
rate and growth of the orchid Guarianthe skinnerii when inoculated
with or without T. harzianum during acclimatizing of the seedlings
obtained after micropropagation. The G. skinnerii seedlings were
obtained by in vitro germination of seeds from the capsule. Seedlings
were transferred to unicel pots contained peat moss and were inoculated
with T. harzianum. A treatment without inoculation served as control.
The seedlings were kept in climatic chamber at 20 ± 2 ° C for
4 months (step one), in the laboratory at 25 ± 8 °C for four
months (step two) and in the greenhouse at 30 ± 12 °C for four
months (step three). The seedlings were distributed according to a completely
aleatorized experimental design. After each step, the percentage of plants
that survived, plant height, number of leaves and shoots were determined.
Survival of G. skinnerii inoculated with T. harzianum was
significantly 1.6 times higher than those non-inoculated seedlings. Plant
height of inoculated plants was 3.6 times greater than control plants
after the first step. The number of leaves was 2.5 times higher only after
the step one. The amount of shoots was 1.4 times higher in the seedlings
inoculated with T. harzianum. The results indicated that the survival
and growth of seedlings inoculated with T. harzianum increased
in extreme temperature conditions for acclimatization.]]>Arditti, J. and A.K.A. Ghani,2000145367421Batty, A.L., M.C. Brundrett, K.W. Dixon and K. Sivasithamparam,200654367374Chase, M.W., K.M. Cameron, R.L. Barrett and J.V. Freudenstein,20032003pp: 69-89pp: 69-89El-Hasan, A., F. Walker, and H. Buchenauer,2008Trichoderma harzianum and its metabolite 6-pentyl-alpha-pyrone suppress fusaric acid produced by Fusarium moniliforme.]]>1567987Murashige, T. and F. Skoog,196215473497Ozbay, N., and S.E. Newman,2004Trichoderma harzianum strains to colonize tomato roots and improve transplant growth.]]>7253257Ozbay, N. and S.E. Newman,2004Trichoderma spp. With emphasis on T. harzianum.]]>7478484 Posposilova, J., I. Ticha, P. Kadlecek, D. Haisel and S. Plzakova,1999ex vitro conditions.]]>42481497Roco, A. and L.M. Perez,2001In vitro biocontrol activity of Trichoderma harzianum on Alternaria alternata in the presence of growth regulators.]]>42232Rudresh, D.L., M.K. Shivaprakash and R.D. Prasad,2005Trichoderma spp. in relation to P uptake and growth and yield parameters of chickpea (Cicer arietinum L.).]]>51217222SAS.,1989Version 6.04 Edn.,Srinath, J., D.J. Bagyraj and B.N. Satyanarayana,2003Ficus benjamina to Glomus mosseae co-inoculated with Trichoderma harzianum and Bacillus coagulans.]]>196972