Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2007.757.764Artocarpus camansi Blanco]]>OccenaIrving V. MojicaElmer-Rico E. MercaFlorinia E. 5200765The aim of this study is to isolate lectin from a seed which had no economical use. A lectin was isolated and purified from the seeds of Artocarpus camansi Blanco by extraction with phosphate buffered saline at pH 7.2. Further purification was done by precipitation with ammonium sulfate at 0-90% saturation. The dialyzed ammonium sulfate precipitate was subjected to gel filtration chromatography using Sephadex G-200 and fractions were tested for lectin activity. Native PAGE was employed to establish the homogeneity of the lectin positive fractions. Fractions with the same band were pooled and lyophilized. The purified lectin is non-blood type specific since it agglutinated all human blood types. It is blood group specific because it agglutinated blood from cow but not from swine. The purified lectin is a complete lectin since it agglutinated erythrocytes without trypsin treatment and addition of metal ions. However, trypsination of erythrocytes increases the agglutination activity. The isolated lectin did not show any specificity towards the ten sugars used in this study. The molecular weight of the purified lectin was estimated by using Sephadex G-200 and was found to be around 2,000 kDa since it eluted in the void volume. The lectin was found to be stable at 20-40°C.]]>Amadeo, G.I., R. Moreira, R. Lima, D. Teixeira, R. Kratje and M. Etcheverrigaray,2003202126Antony, L., D. Basu and P.S. Appukuttan,1989Artocarpus hirsuta): Purification and properties.]]>26361366Aragones, R.C. and F.E. Merca,1998Lablab purpureus Linn. cv. Highworth).]]>1271537Barondes, S.H.,198150207231Barre, A., W.J. Peumans, M. Rossignol, G. Borderies, R. Culerrier, E.J.M. Van Damme and P. Rouge,200486685691Bradford, M.M.,197672248254Chowdhury, S., H. Ahmed and B.P. Chatterjee,1987Artocarpus lakoocha seeds.]]>159137148Cooper, T.,1977De Mesa, M.D., E.R.E. Mojica and F.E. Merca,2004Moringa pterygosperma).]]>291528Gaikwad, S.M. and M.I. Khan,2006Artocarpus hirsuta lectin and jacalin are energetically different.]]>8213151318Ganiko, L., A.R. Martins, E. Freymuller, R.A. Mortara and M.C. Roque-Barreira,20051721152163Goldstein, I.J. and C.E. Hayes,197835127340Goldstein, I.J., R.C. Hughes, M. Monsigny, T. Osawa and N. Sharon,19802856666Gurjar, M.M., M.I. Khan and S.M. Gaikwad,1998Artocarpus hirsuta: Characterization of the sugar specificity and binding site.]]>1381256264Hunter, J.B., M.R. Suresh, A.A. Noujaim, D.S. Hagen, D.H. Heeley and R.G. Micetich,1986Artocarpus altilis) seeds.]]>2319328Kabir, S.,1995Artocarpus heterophyllus (Jackfruit) lectin] based on its charge properties.]]>27147156Kabir, S.,1998Artocarpus heterophyllus) seed-derived lectin of versatile applications in immunobiological research.]]>212193211Lacsamana, M.S. and F.E. Merca,1994Pomacea sp.]]>1237797Laemmli, U.K.,1970227680685Lim, S.B., C.T. Chua and O.H. Hashim,1997Artocarpus integer.]]>209177186Lis, H. and N. Sharon,1986553567Moreira, R.A., C.C. Castelo-Branco, A.C.O. Monteiro, R.O. Tavares and L.M. Beltramini,1998Artocarpus incisa L. seeds.]]>4711831188Mourao, R.H.V., F.J. Xavier, E.W. Alves, E.G. Orellano and D. Fernandes de Melo et al.,1999Artocarpus heterophyllus seeds.]]>184147Pancho, J.V.,1979pp: 116-117pp: 116-117Peumans, W.J., B. Hause and E.J.M. Van Damme,2000477186192Scopes, R.K.,1994Sharon, N.,1977236108119Silva-Lucca, R.A., M. Tabak, O.R. Nascimento, M.C. Roque-Barreira and L.M. Beltramini,1999Artocarpus integrifolia seeds.]]>798193Smetana, K. Jr. B. Dvorankova, M. Chovanec, J. Boucek and J. Klima et al.,2006125171182Teixeira, S.A., M.S. Viapiano, L. Ganiko, M.C. Roque-Barreira and A.R. Martins,200420501508Tran, T.P.L., V.H. Nguyen, T.Q. Nguyen and N.L. Do,2006Artocarpus champeden (Mit to nu), A. heterophyllus Lamk (mit dai) and A. masticata (mit chay).]]>461618Verheij, E.W.M. and R.E. Coronel,1982pp: 103-106pp: 103-106Wu, A.M. and S. Sugii,1991213127143Zhang, C., S. Zhou, Y. Wu, Y. Deng and D. Zhou,1999Artocarpus lingnanensis.]]>15142144