Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2007.419.422Cymbopogon flexuosus Ness Ex. Steud) Wats Cultivar OD-19]]>LuthraRajesh SrivastavaAshok K. GanjewalaDeepak 2200762The aerial parts (leaves) of lemongrass (Cymbopogon flexuosus Nees ex Steud) wats (cultivar OD-19) on steam-distillation yield an essential oil rich in acyclic monoterpenes, citral (83%). To specifically locate the sites of citral accumulation in lemongrass we employed Schiffs reagent, which upon reaction with aldehydes (citral) gives a purple-red colouration. Using this technique, single oil-accumulating cells were detected in the adaxial side of leaf mesophyll commonly adjacent to non-photosynthetic tissue and between vascular bundles. In addition to cultivar OD-19, a citral lacking cultivar GRL-1 (geraniol rich) leaf sections, also stained with schiffs reagent and compared with that of lemongrass cultivar OD-19 leaf sections. In lemongrass mutant GRL-1, these specialized cells, however, could not be stained due to lack of citral. Hence, it is confirm that the observed schiffs staining is associated with accumulated citral.]]>Bouvier, F., C. Surie, A. D`Harlingue, R.A. Backhaus and B. Camara,200024241252Brubaker, C.L., C.G. Benson, C. Miller and D.N. Leach,1996Gossypium species.]]>44601612Croteau, R. and M.A. Johnson,19841984pp: 135-185pp: 135-185Croteau, R.,19861986pp: 81-133pp: 81-133Fahn, A.,1988108229257Gang, D.R., J. Wang, N. Duderva, K.H. Nam, J.E. Simon, E. Lewinsohn and E. Pichersky,2001125539555Gersbach, P.V., S.G. Wyllie and V. Sarafis,200188521525Guenther, E.,1950pp: 20-25pp: 20-25Husain, A.,19941994Iruthayathas, E.E. and H.M.V. Herath,198259227230Lewinsohn, E., N. Dudai, Y. Tadmor, I. Katzir, U. Ravid, E. Putievsky and D.M. Joel,1998Cymbopogon citratus (DC.) Stapf., Poaceae).]]>813539Lichtenthaler, H.K., M. Rohmer and J. Schwender,1997101643652Luthra, R., P.M. Luthra and S. Kumar,199976133135McKaskill, D. and R. Croteau,199755107146Ming, L.C., R.O. Figueiredo, S.R. Machado and R.M.C. Andrade,1996Cymbopogon citratus (DC) Stapf poaceae.]]>426555559O`Brien, T. and M. McCully,1981Rhomer, M., M. Knani, P. Simonin, Sutter and H. Sahm,1993295517524Tholl, D., C.M. Kish, I. Orlova, D. Sherman, J. Gershenzon, E. Pichersky and N. Dudareva,2004Antirrhinum majus and Clarkia breweri flowers involves heterodimeric geranyl diphosphate synthase.]]>16977992Tsai, Y.,1978Cymbopogon flexuosus Stapf) leaf.]]>1037580