Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2007.36.41in vitro Culture of Some Deepwater Rice (Oryza sativa L.) Cultivars]]>KhaledaL. AhmedA.M.A. MarzanL.W. Al-ForkanM. 1200761The experiment was conducted to identify the in vitro responsiveness for callus induction along with plant regeneration of deepwater rice in the presence of NaCl. In this study, callus induction and plant regeneration responsiveness were tested using different concentrations of 0.1-0.3% (w/v) NaCl which added in MS and LS based media. Marked variation was observed both in calli proliferation and plant regeneration among the six deepwater rice cultivars. In the presence of 0.1% (w/v) NaCl, the highest number of callus induction in all genotypes recorded on the media supplemented with 2 mg L-1 2,4-D + 0.1% (w/v) NaCl. The highest dose of NaCl salt 0.3% (w/v) inhibited callus induction compared to 0.1 and 0.2% NaCl media combinations. Among the cultivars tested, cv. Murabajal produced the highest percentage (39%) of callus whilst cvs. Gheoch (23%) and BR224-2B-2-5 (8%) responded poorly in terms of callus production on the MS based 0.1% NaCl supplemented medium. NaCl had no clear promotive effect on regeneration percentage. In this study, green plant did not regenerate from cv. HA-8. Salinity strongly reduced regenerating capacities of callus obtained from all the cultivars. The mean number of shoots produced per regenerating calli was gradually decreased in all the cultivars when the concentration of NaCl increased in the medium. The apparent tolerance of NaCl in these deepwater rice cultivars may be at least partially related to its growth rate since it can dilute the contents of ion in the shoot. The results of the present study showed the decreasing trend in callus proliferation and plant regeneration with the increasing concentrations of NaCl.]]>Abe, T. and Y. Futsuhara,199166129140Adam, P., J.C. Thomas, D.M. Vernon, H.J. Bohner and R.G. Jensen, 19923312151223Binch, D.Q., L.E. Heszky, G. Gyulai and A. Csillag,1992Oryza sativa L.) in high saline conditions.]]>297582Bong, B.B., A. Tobita and T. Senboku,1996213838Karim, Z., S.G. Hussain and M. Ahmed,19901990Kishor, P.B.K., S. Sangam and K.P. Naidu,19991999pp: 7885Lee, S.Y., J.H. Lee and T.O. Kwon,200374143149Linsmaier, E.M. and F. Skoog,196518100127Lutts, S., J.M. Kinet and J. Bouharmont,1996O. sativa L.) cultivars differing in salinity resistance.]]>19207218Lutts, S., J.M. Kinet and J. Bouharmont,199957311Moradi, F., A. Ismail, G. Gregrio and J. Egdane,20038105116Murashige, T. and F. Skoog,196215473497Peng, J. and T.K. Hodges,1989Oryza sativa L.). In vitro]]>259194Sathish, P., O.L. Gamborg and M.W. Nabors,1997+/Na+ in callus and plant cells.]]>19512031209Subhashini, K. and G.M. Reddy,1998In vitro selection for salinity and regeneration of plants in rice.]]>58584586Torrizo, L.B. and F.J. Zpata,19865136139Vajrabhaya, M., T. Thanapaisal and T. Vajrabhaya,19898411414Yeo, A.R. and T.J. Flowers,1986Oryza sativa L.) and pyramiding approach to breeding for saline soils.]]>13161173