Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2007.1137.1142Ustilago scitaminea Using Specific Primers]]>Mahin B. Izadi S. Ali Moosawi-Jorf 7200767Teliospores of Ustilago scitaminea were collected from whips of infected sugarcanes and cultured on YGC medium which conducted to isolation of yeast-like and mycelial colonies. White mycelia were also isolated from culturing of pieces of infected tissues of sugarcane. Results of PCR using specific primers bE4 and bE8 revealed the fungal detection in extracted DNA from yeast-like and mycelial colonies. PCR reaction was specifically amplified a DNA fragment of 495 bp in all three types of colonies of yeast-like and mycelial forms. This DNA fragment was not amplified in DNA extraction of yeast-bacterial colonies and other saprophytic-mycelial fungi.]]>Agnitori, V.P.,1990Albert, H.H. and S. Schenck,1996Ustilago scitaminea DNA.]]>8011891192Alexander, K.C. and R.K. Krishna,1978Ustilago scitaminea) of sugarcane: Longevity and viability of teliospores.]]>14749Alizadeh, A., S.A. Moosavi-Jorf and R. Farrokhi-Nejad,2001Tilletia lindica: Teliospore germination to teliospore formation, in vitro and in vitro.]]>37233245Chona, B.L.,19434401404Ershad, D. and N. Bani-Abbassi,197171922Fereol, L.,1984in vitro cultivated sugarcane with smut (Ustilago scitaminea).]]>6220432046McMartin, A.,1945295557Moosawi-Jorf, S.A., M. Teymour-Zadeh and M. Izadi,2006Ustilago scitaminea, the causal agent of sugarcane smut.]]>2006pp: 256Moosawi-Jorf, S.A. and M.B. Izadi,2007In vitro detection of yeast-like and mycelial colonies of Ustilago scitaminea in tissue-cultured plantlets of sugarcane using polymerase chain reaction.]]>737683773Murray, M.G. and W.F. Thompson,1980843214326Pross, J.P. and P. Chagrardieff,1983in vitro effects toxiques entre Ustilago scitaminea Syd.]]>3629634Ricaud, C., B.T. Egan, A.G. Gillaspiejr and C.G. Hughes,1989pp: 339pp: 339Schenck, S.,1998625Shaif-Nabi, B., D.K. Agarwal and N. Mitter,2001 Neovossia indica by RAPD-PCR and clustering based on teliospore morphology.]]>36223236Singh, N., B.M. Somai and D. Pillay,2004167987994Sinha, O.K. and K. Singh,1983Fusarium moniliforme var. subglutinas against sugarcane smut.]]>369294Vanky, K.,199991357359