Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2006.139.141in vitro Germination of Embryos from Taxus media Rehder]]>Zhihua Liao Yifu Gong Yan Pi Min Chen Qiumin Tan Feng Tan Xiaofen Sun Kexuan Tang 1200651An efficient protocol was established to obtain seedlings of Taxus media Rehder by embryo culture technique. No seedlings of T. media were obtained without the excision of endosperm and few embryos were able to germinate on half-strength MS media in the absence of plant growth regulators, so it was necessary for embryo germination to get rid of endosperms of the mature seeds and to add plant growth regulators to prompt embryo germination. The effects of three factors, namely BA, GA3 and 2,4-D, on embryo germination were examined by L9(34) orthogonal design, the orthogonal array that corresponds to four levels and three parameters in nine treatments. The analysis of variance of the results of orthogonal tests showed that half-strength MS media supplemented with BA 1.0 mg L-1, 2,4-D 0.1 mg L-1 were the optimized media for embryo germination of Taxus media. On the optimized media, 62% of the embryos could germinate and develop into seedlings.]]>Wani, M.C., H.L. Taylor, M.E. Wall, P. Coggon and A.T. Mcphail,1971Taxus brevifolia. ]]>9323252327Rowinsy, E.K., L.A. Cazenave and R.C. Donehower, 19908212471259Miller, R.W.,1980Taxus alkaloids and other taxane derivatives. ]]>43425437Flores, T., L.J. Wagner and H.E. Flores,1993Taxus spp. in vitro.]]>29160165Flores, H. and P.J. Sgrignoli,1991In vitro culture and precocious germination of Taxus embryos.]]>27139142Chang, Sh. and J. Yang,1996Taxus mairei using suitable culture medium and PVP.]]>373540Anderson, E.D. and J.N. Owens, 2001Taxus brevifolia. ]]>3110461056Hu, L.Y., L. Wang and B. Wu, 1992In vitro culture of immature Taxus embryos. ]]>27210210Zhiri, A., M. Jaziri, J. Homes, M. Vanhaelen and K. Shimomura, 1994in vitro rapid germination of Taxus embryos and the evaluation of taxol content in the plantlets.]]>39261263Chee, P.P.,1994In vitro culture of zygotic embryos of Taxus species.]]>29695697Murashige, T. and F. Skoog,196215473497Ross, P.J., 1989pp: 88pp: 88Chien, C.T., J.J. Yang, Y.I. Chuang and T.P. Lin, 1994Taxus mairei seed.]]>204647Le Page-Degivry, M.T.,1968Taxus baccata L. the title in english The evidence of dormancy associated to the immaturity of the embryos of Taxus baccata L. ]]>26610281030Le Page-Degivry, M.T., 1973Taxus baccata L. (Intervention of inhibitor in the embryonic dormancy of Taxus baccata L.). ]]>277177180