Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2004.675.678Kaempferia galanga L.]]>RahmanM.M. M.N. Amin T. Ahamed M. R. Ali A. Habib 6200436Somatic embryogenesis and subsequent plantlets regeneration were achieved in callus cultures established from the leaf base explants of Kaempferia galanga L. Callus induction and somatic embryogenesis at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed on MS medium supplemented with 1.5 mg L-1 2,4-D+1.0 mg L-1 BA. After transfer on MS medium supplemented with 2.0 mg L-1 BA+0.1 mg L-1 NAA, this callus produced small globular embryos first that later developed into plantlets by further subculturing on the same medium. Plantlets were acclimatized and subsequently transferred to the field. Survival rate of the plantlets under ex vitro condition was 85%.]]>Sadimann, J.,1992Kaempferia galanga L.).]]>47636639Wong, K.C., K.S. Ong and C.L. Lim,1992kaempferia galanga L.]]>7263266Kackar, A., S.R. Bhat, K.P.S. Chandel and S.K. Malik,199332289292Malamug, J.J.F., H. Inden and T. Asahira,1991488997Haque, M.I., S. Perveen and R.H. Sarker,1999In vitro propagation of ginger (Zingiber officinale Rosc.).]]>94451Amin, M.N., M.A. Islam and M.A.K. Azad,2001Alphinia calcarata Rosc.]]>2001pp: 5555Vincent, K.A., M. Bijoy, M. Hariharan and K.M. Mathew,1991Kaempferia galanga L.]]>34396400Vincent, K.A., M. Hariharan and K.M. Mathew,1992Kaempferia galangal L.]]>42253256Murashige, T. and F. Skoog,196215473497Mathukumar, B., D.I. Arokiasasamy and S. John Britto,2000In vitro propagation of Datura metel L. from hypocotyls explants.]]>103944Haque, M.S., T. Wada and K. Hattori,2000Allium salmon L.).]]>10157166Abo El-Nil, M.M.,1997Allium sativum L).]]>9259264Rout, G.R., S. Samantaray and P. Das,1996In vitro somatic embryogenesis and plant regeneration in callus culture of Muntingia calabura L.]]>61524Begum, S., L. Hakim and M.A. Azam,1995515Kirtikar, K.R. and B.D. Basu,1996Vol. 5,pp: 2422-2423pp: 2422-2423Ghani, A.,1998pp: 290-291pp: 290-291Parvin, S., M. Hossain, M.A. Bari, S. Huda and M.S. Islam,1999In vitro plant regeneration in cardamom (Elettaria cardimomum M.).]]>1999pp: 4747