Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2003.585.592Phytophthora infestans Hyphal Cell Wall Component and Suppressor Signal
Transduction]]>Amjad Hassan Miki Kato Noriyuki Hatsugai Tomoo Okuta Takayuki Matsuura Akiyoshi Miyazaki Michi Matsubara Kazutoshi Yokokawa Hideaki Oikawa Naotaka Furuichi 8200328We investigated localization of calcium-dependent protein kinase (CDPK) in potato microsomal fraction (MF) and its role in relation to hyphal cell wall component (HWC) and suppressor glucan (suppressor) of Phytophthora infestans (Mont.) de Bary in hypersensitive resistance response (HR). Additionally, we studied activity of CDPK from MF in the absence and presence of staurosporine, a kinase inhibitor and okadaic acid, a phosphatase inhibitor. Stimulation of MF kinase activity by the addition of Ca2+ supported the presence of CDPKs in MF. This was confirmed when a 70 kDa CDPK was detected in potato MF using western immunoblot analysis. HWC and suppressor stimulated kinase activity of MF within 5-10 min after application. In the presence of staurosporine, HWC stimulated MF kinase activity but suppressor did not. In the presence of okadaic acid, HWC inhibited and suppressor stimulated kinase activity of MF. These results suggest that HWC and suppressor may interact with potato kinase(s) in the MF, initiating a signal leading to the occurrence of HR, or in the case of suppressor, to the inhibition of HR. We suggest that the signal cascades involving HWC and suppressor from P. infestans may be different in incompatible and compatible interactions, respectively, occurring between potato and P. infestans.]]>Doke, N.,1983Phytophthora infestans and to the hyphal wall components.]]>23345357Doke, N. and Y. Miura,1995In vitro activation of NADPH-dependent O2-generating system in a plasma membrane-rich fraction of potato tuber tissues by treatment with an elicitor from Phytophthora infestans or with digitonin.]]>461728Doke, N. and K. Tomiyama,1980Phytophthora infestans on protoplasts of potato tuber tissues.]]>16169176Furuichi, N. and J. Suzuki,1989Phytophthora infestans.]]>127281290Furuichi, N., M. Kato, N. Takamura, E. Terada and T. Kato,1997Phytophthora infestans: Isolation of proteins related to generation of active oxygen species in the microsomal fraction of potato tubers.]]>454145Garas, N.A., N. Doke and J. Kuc,1979Phytophthora infestans.]]>15117126Harmon, A.C., M. Gribskov and J.F. Harper,20002+ signal?]]>5154159Harmon, A.C., C. Putnam-Evans and M.J. Cormier,198783830837Klimczak, L.J. and J. Hind,199092919923Klucis, E. and G.M. Polya,198888164171Laemmli, U.K.,1970227680685McAinsh, M.R. and A.M. Hetherington,19982+ signaling systems.]]>33236Romeis, T., A.A. Ludwig, R. Martin and J.D.J. Jones,20012055565567Romeis, T., P. Pedro and J.D.G. Jones,200012803815Schaller, G.E. and M.R. Sussman,1988+-ATPase of oat roots by a calcium-stimulated protein kinase.]]>173509518Scheel, D.,19981305310Suganuma, M. and H. Fujiki,19933819601970Suganuma, M., H. Fujiki, S. Okabe, S. Nishiwaki, D. Brautigan, T.S. Ingebritsen and M.R. Rosner,199230873878Suzuki, Y.S., Y. Wang and J.Y. Takemoto,1992+-ATPase from red beet storage tissue.]]>9913141320Towbin, H., T. Staehelin and J. Gordon,19797643504354Xing, T., X. Wang, K. Malik and B.L. Miki,2001Arabidopsis calmodulin-like domain protein kinase-enhanced NADPH oxidase activity and oxidative burst in tomato protoplasts.]]>1412611264Yanagihara, N., E. Tachikawa, F. Izumi, S. Yasugawa, H. Yamamoto and E. Miyamoto,19912+/calmodulin-dependent protein kinase.]]>56294298Yang, Y.O., J. Shah and D.F. Klessig, 19971116211639Yuzuru, M.,19933819381949Zimmermann, S., T. Nurnberger, J.M. Franchisse, W. Wirtz, J. Guern, R. Hedrich and D. Scheel,19972+-permeable ion channel involved in pathogen defense.]]>9427512755Furuichi, N., A.J. Anderson, Y. Suzuki and J.Y. Takemoto,1994Phytophthora infestans Stimulate Phosphorylation of Plasma Membrane Proteins from Potato and Bean Tissues.]]>1994pp: 273-274pp: 273-274Furuichi, N., Y. Suzuki, M. Matsubara, M. Aoyagi and N. Takamura,1998Phytophthora infestans in Potato Plasma Membrane by Using Surface Plasmon Biosensor.]]>1998pp: 367-368pp: 367-368Hernandez, H.A. M., L.A. Sellers and A. Aitken,19911991pp: 331-335pp: 331-335