Asian Journal of Plant Sciences1682-39741812-5697Asian Network for Scientific Information10.3923/ajps.2003.425.430BhowmikPankaj Kumar Toshiyuki Matsui Haruo Suzuki Yusuke Kosugi 5200325An asparagus (Asparagus officinalis L. cv. Welcome) cDNA clone coding the enzyme phenylalanine ammonia-lyase (PAL) was isolated from a cDNA library prepared from asparagus spears using reverse transcription- polymerase chain reaction (RT-PCR). The partial cDNA clone encodes an mRNA of 527 bp and the derived amino acid sequence is highly homologous to PAL from rice, maize and barley. Northern blot analysis showed increase level of pAS-ACO mRNA until 24 hour at 200C, which coincided well with PAL activity and fiber development, suggesting that the increase might be a response to the wounding associated with harvest.]]>Campbell, M.M. and B.E. Ellis,1992186409417Campbell, M.M. and B.E. Ellis,199231737742Edwards, K., C.L. Cramer, G.P. Bolwell, R.A. Dixon, W. Schuch and C.J. Lamb,19858267316735Engelsma, G.,196882355368Fritzemeier, K.H., C. Cretin, E. Kombrink, F. Rohwer, J. Taylor, D. Scheel and K. Hahlbrock,1987Phytophthora infestans.]]>853441Goldstein, L.D., P.H. Jennings and H.V. Marsh, Jr.,197213783793Gross, G.G.,198182563Higuchi, T.,196630667673Imaseki, H., M. Uchiyama and I. Uritani,196832387389Isherwood, F.A.,19631963pp: 300-303pp: 300-303Kato, M., Y. Hayakawa, H. Hyodo, Y. Ikoma and M. Yano,2000Cucurbita maxima.]]>41440447Lawton, M.A. and C.J. Lamb,19871335341Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall,1951193265275Orr, J.D., R. Edwards and R.A. Dixon,1993Medicago sativa L.). XIV. Changes in the level of phenylpropanoid pathway intermediates in relation to regulation of L-phenylalanine ammonia-lyase in elicitor treated cell-suspension cultures.]]>101847856Rubery, P.H. and D.E. Fosket,1969875462Wan, C.Y. and T.A. Wilkins,1994Gossypium hirsutum L.).]]>223712Yoshida, S. and M. Shimokoriyama,1965781419