Asian Journal of Plant Pathology1819-1541xxxx-xxxxScience International10.3923/ajppaj.2020.1.10Botrytis spp. Infected Ornamental Plants and Emergence of Resistant Isolates Against Fenhexamid in Egypt]]>WahabHala Abdel WagihElsayed E. ShehataMohamed R.A. FahmyMagda M. GaberMahmoud A. 12020141Background and Objective:Botrytis is a serious fungal pathogen which infects most ornamental plants and its resistance to effective fungicide is a continuous challenge facing disease management. The current research aimed to identify resistant Botrytis spp. from different ornamental plants against fenhexamid. Materials and Methods: About 64 single-spore isolates were collected from different symptomatic and asymptomatic ornamental plants using the modified Kerssies medium, m1KERS. Phenotypic variability was shown in some features including growth texture, sclerotia pattern and conidial dimensions. Pathogenicity and mycelial growth rate tests were statistically carried out using one way analysis of variance (ANOVA). Molecular characterisation was demonstrated by transposable elements (TEs) analysis and fenhexamid resistance was revealed using mycelial growth assay. Results: All isolates were identified as Botrytis spp. according to their morphological and molecular characteristics. Based on phylogenetic analysis, BCLi1 isolate seemed to be distantly related to B. cinerea and other Botrytis species. Significant variation in aggressiveness among isolates was observed on detached lettuce leaves. Four TE genotypes, transposa, boty, flipper and vacuma, were detected in Botrytis populations with TE distribution reached 50, 29.7, 10.9 and 9.4%, respectively. The low virulence level of some Botrytis spp. isolates seemed to be related to vacuma isolates suggesting a possible correlation between virulence and TE genotype. Moreover, a low to moderate resistance of Botrytis spp. isolates was revealed towards the hydroxyanilide fungicide, fenhexamid, in three transposa isolates, BCT6, BCGL2 and BCGL5, with a mean EC50 value of 0.2, 0.3 and 0.5 μg mL1, respectively. Conclusion: The results demonstrated the first observation of the emergence of resistant isolates of Botrytis from ornamental plants against the highly effective fungicide in Egypt, providing a serious attention towards Botrytis management.]]>Elad, Y., B. Williamson, P. Tudzynski and N. Delen,2007Botrytis spp. and Diseases They Cause in Agricultural Systems-An Introduction.]]>2007pp: 1-8pp: 1-8Elad, Y., I. Pertot, A.M.C. Prado and A. Stewart,2016Botrytis spp.]]>2016pp: 413-486pp: 413-486Carisse, O.,2016Botrytis spp.]]>2016pp: 127-148pp: 127-148Dufresne, M., A. Hua-Van, H. Abdel Wahab, S.B. M'Barek and C. Vasnier et al.,2007175441452Lopez-Berges, M.S., A. Di Pietro, M.J. Daboussi, H. Abdel Wahab and C. Vasnier et al.,2009Fusarium oxysporum f. sp. lycopersici by large-scale transposon tagging.]]>1095107Abdel Wahab, H.,2015Botrytis cinerea isolates from different host plants.]]>5177189Aboelghar, M., M.S. Moustafa, A.M. Ali and H. Abdel Wahab,2019Botrytis cinerea infected lettuce.]]>52642Wagih, E.E., H. Abdel Wahab, M.R.A. Shehata, M.M. Fahmy and M.A. Gaber,2019Botrytis cinerea isolates infecting grape and strawberry in Egypt.]]>83751Fan, F., M. Hahn, G.Q. Li, Y. Lin and C.X. Luo,2019Botrytis cinerea by loop-mediated isothermal amplification.]]>2019Abdel Wahab, H. and N.A.S. Helal,2013Botrytis infections.]]>76469Moller, E.M., G. Bahnweg, H. Sandermann and H.H. Geiger,19922061156116Rigotti, S., K. Gindro, H. Richter and O. Viret,2002Botrytis cinerea Pers.: Fr. in strawberry (Fragaria × ananassa Duch.) using PCR.]]>209169174White, T.J., T.D. Bruns, S.B. Lee and J.W. Taylor,19901990pp: 315-322pp: 315-322Tamura, K. and M. Nei,199310512526Kumar, S., G. Stecher, M. Li, C. Knyaz and K. Tamura,20183515471549Felsenstein, J.,198539783791Diolez, A., F. Marches, D. Fortini and Y. Brygoo,1995Boty, a long-terminal-repeat retroelement in the phytopathogenic fungus Botrytis cinerea.]]>61103108Levis, C., D. Fortini and Y. Brygoo,1997Flipper, a mobile fot1-like transposable element in Botrytis cinerea.]]>254674680Leyronas, C., F. Bryone, M. Duffaud, C. Troulet and P.C. Nicot,2015Botrytis cinerea on lettuce and tomato by genotypic and phenotypic characterization.]]>64119127Ma, Z. and T.J. Michailides,2005Botrytis cinerea from different hosts in California. ]]>8910831089Karchani-Balma, S., A. Gautier, A. Raies and E. Fournier,2008Botrytis cinerea Populations.]]>9812711279Vaczy, K.Z., E. Sandor, L. Karaffa, E. Fekete and E. Fekete et al.,2008Botrytis cinerea populations in Hungarian vineyards.]]>9813121319Johnston, P.R., K. Hoksbergen, D. Park and R.E. Beever,2014Botrytis in New Zealand vineyards and the significance of its seasonal and regional variation.]]>63888898Amiri, A., A.I. Zuniga and N.A. Peres,2018Botrytis cryptic species in strawberry nursery transplants and strawberry and blueberry commercial fields in the Eastern United States.]]>102398404Zhang, Y., X. Li, F. Shen, H. Xu, Y. Li and D. Liu,2018Botrytis cinerea isolates from grape vineyards in China.]]>1024048Samuel, S., T. Veloukas, A. Papavasileiou and G.S. Karaoglanidis,2012Botrytis cinerea populations from several hosts in Greece.]]>9612861290Kumari, S., P. Tayal, E. Sharma and R. Kapoor,2014Botrytis cinerea isolates.]]>169862872Giraud, T., D. Fortini, C. Levis, C. Lamarque, P. Leroux, K. Lobuglio and Y. Brygoo,1999Botrytis cinerea complex, transposa and vacuma, are found in sympatry on numerous host plants.]]>89967973Muñoz, G., P. Hinrichsen, Y. Brygoo and T. Giraud,2002Botrytis cinerea populations in Chile.]]>106594601Martinez, F., B. Dubos and M. Fermaud,2005Botrytis cinerea in Vineyards.]]>95692700Kretchmer, M. and M. Hahn,2008Botrytis cinerea isolates from a vineyard in Germany.]]>115214219Esterio, M., G. Muñoz, C. Ramos, G. Cofré, R. Estévez, A. Salinas and J. Auger,2011Botrytis cinerea isolates present in thompson seedless table grapes in the central valley of Chile.]]>95683690Fournier, E., T. Giraud and Y. Brygoo,2005Botrytis cinerea complex in France using multiple gene genealogies.]]>9712511267Isenegger, D.A., P.K. Ades, R. Ford and P.W.J. Taylor,2008Botrytis cinerea species complex and microsatellite analysis of transposon types in South Asia and Australia.]]>291727Martinez, F., M.F. Corio-Costet, C. Levis, M. Coarer and M. Fermaud,2008Botrytis cinerea populations in French Vineyards.]]>47217226Muñoz, C., S.G. Talquenca, E. Oriolani and M. Combina,2010Botrytis cinerea isolates from Argentina.]]>276670Rajaguru, B.A.P. and M.W. Shaw,2010Botrytis cinerea in Southern England.]]>5910811090Pande, S., M. Sharma, G.K. Kishore, L. Shivram and U.N. Mangala,2010Botrytis cinerea isolates from chickpea: DNA polymorphisms, cultural, morphological and virulence characteristics.]]>979617967Albertini, C., G. Thebaud, E. Fournier and P. Leroux,2002Botrytis cinerea.]]>10611711178Saito, S., D. Margosan, T.J. Michailides and C.L. Xiao,2016Botrytis californica, a new cryptic species in the B. cinerea species complex causing gray mold in blueberries and table grapes.]]>108330343Kecskeméti, E., A. Brathuhn, K.H. Kogel, B. Berkelmann-Löhnertz and A. Reineke,2014Botrytis cinerea isolates collected from a German grapevine growing region.]]>162582595Saito, S., T.J. Michailides and C.L. Xiao,2014Botrytis pseudocinerea causing gray mold on blueberry in North America.]]>9817431743Zhang, W., J.A. Corwin, D. Copeland, J. Feusier and R. Eshbaugh et al.,2017Botrytis pathosystem.]]>2927272752Zhou, Y.J., J. Zhang, X.D. Wang, L. Yang and D.H. Jiang et al.,2014Botrytis sinoviticola, a novel cryptic species causing gray mold disease of table grapes (Vitis vinifera) in China.]]>1064356