Asian Journal of Plant Pathology1819-1541xxxx-xxxxScience International10.3923/ajppaj.2016.29.35Allium cepa L.) in South India]]>AsadhiSujitha VeeraBhaskara Reddy Bommu YeturuSivaprasad RayalcheruvuUsha 32016103Background and Objective: Groundnut Bud Necrosis Virus (GBNV) (family Bunyaviridae , genus Tospovirus) is an emerging plant viral disease. The GBNV was a very broad host range infecting many economically important crops throughout in India. So the aim of this study is to survey, screening, identification of GBNV infecting onion to know the genetic diversity and compare the sensitivity limit of ELISA, RT-PCR and IC-RT-PCR in GBNV infected onion samples. Materials and Methods: The straw colored, mosaic and necrotic lesions exhibiting by young leaves of onion plants were collected from different locations in South India. The disease samples (No. of samples = 73) were initially screened by DAC-ELISA by using the GBNV coat protein polyclonal antibodies. Total RNA was isolated from the positive ELISA samples and amplified with GBNV coat protein gene specific primers. Comparison of the sensitivity limit of ELISA, RT-PCR and IC-RT-PCR in GBNV infected onion samples. Results: In DAC-ELISA, 50 (68.49%) samples were confirmed as GBNV infected from collected onion samples (n = 73) in the field. In RT-PCR, 61 samples (83.56%) were confirmed by RT-PCR method and 68 samples (93.15%) were confirmed by IC-RT-PCR based on the coat protein gene of GBNV. The sequence analysis revealed that the coat protein gene shared 93-100 and 95-100% sequence identity with GBNV at the nucleotide and amino acid levels, respectively. The sensitivity was compared in DAC-ELISA, RT-PCR and IC-RT-PCR showed 103, 105and 106dilutions, respectively and not observed in healthy onion samples. Conclusion: The IC-RT-PCR was found to be more sensitive than RT-PCR and ELISA for the detection of GBNV.]]>NHB.,20152015Ross, I.A.,2001Vol. 2,Pages: 487Pages: 487Maliogka, V.I., C.I. Dovas, D.E. Lesemann, S. Winter and N.I. Katis,2006Artichoke yellow ringspot virus infecting onion crops.]]>96622629Mumford, R.A., I. Barker and K.R. Wood,1996128159183Ravi, K.S., A.S. Kitkaru and S. Winter,200655288288Sujitha, A., B.V. Bhaskara Reddy, Y. Sivaprasad, R. Usha and D.V.R. Sai Gopal,2012Groundnut bud necrosis virus infecting onion (Allium cepa).]]>7183187Reddy, D.V.R., A.A.M. Buiel, T. Satyanarayana, S.L. Dwivedi and A.S. Reddy et al.,19951995Reddy, D.V.R. and K.T. Devi,20032003Clark, M.F. and M.B. Joseph,19841984pp: 51-58pp: 51-58Thompson, J.D., D.G. Higgins and T.J. Gibson,19942246734680Page, R.D.M.,199612357358Benson, D.A., M.S. Boguski, D.J. Lipman, J. Ostell, B.F.F. Ouellette, B.A. Rapp and D.L. Wheeler,1999271217Satyanarayana, T., S.E. Mitchell, D.V.R. Reddy, S. Kresovich and R. Jarret et al.,19967723472352Prins, M., M. Kikkert, C. Ismayadi, W. de Graauw, P. de Haan and R. Goldbach,1997Tomato spotted wilt virus in transgenic tobacco plants expressing NSM gene sequences.]]>33235243Satyanarayana, T., S. Gowda, K.L. Reddy, S.E. Mitchell, W.O. Dawson and D.V.R. Reddy,1998143353364Dong, J.H., Y.Y. Yin, Q. Fang, J.H. McBeath and Z.K. Zhang,2013Hippeastrum sp. in China.]]>46567570Bhat, A.I., R.K. Jain, A. Varma, N. Chandrn and S.K. Lal,200154112116Thien, H.X., A.I. Bhat and R.K. Jain,200311110116Jain, R.K., S.M.P. Khurana, A.I. Bhat and V. Chaudhary,2004Groundnut bud necrosis virus.]]>57169173Satyanarayana, T., S.E. Mitchell, D.V.R. Reddy, S. Brown and S. Kresovich et al.,19961418598Sivaprasad, Y., B.V.B. Reddy, C.V.M.N. Kumar, K.R. Reddy and D.V.R. Sai Gopal, 2011Corchorus capsularis): A new host of Peanut bud necrosis virus.]]>233333Sivaprasad, Y., B.V.B. Reddy, C.V.M.N. Kumar, K.R. Reddy and D.V.R. Sai Gopal,2011Colocasia esculenta).]]>63032Hobbs, H.A., D.V.R. Reddy, R. Rajeswari and A.S. Reddy,198771747749