Asian Journal of Plant Pathology1819-1541xxxx-xxxxScience International10.3923/ajppaj.2016.1.8IlsanNoor Andryan NawangsihAbdjad Asih WahyudiAris Tri 1-22016101-2Bacterial Leaf Blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is the most destructive and serious disease causing productivity loss, especially in rice, in Asia. The chemical pesticide usage over a long period of time brings about adverse toxic effect on the potential production of the land and the main consumers of the products. Microbes as biocontrol agents have efficiency and safety for humans and other non-target organisms. They leave small amount or no residue in foods. Soil microbes cannot be used directly as biopesticides because common root-colonizers fail to establish on leaves. Actinomycetes are Gram positive bacteria that are known to produce bioactive compound up to 70% of the total compound produced by bacteria, including antimicrobes. Several strains of actinomycetes are known capable of protecting plants against plant disease. The aims of this study were to isolate and screen non-pathogenic phyllosphere actinomycetes of rice which are capable of controlling BLB disease in rice. A total eight isolates are positively capable of controlling Xoo in vitro and four isolates significantly reduce disease severity of BLB. Pellet of STG 15, which is the best, showed 25.87% control efficiency of BLB severity at 14 Days After Inoculation (DAI) using Xoo compared to control. Molecular identification based on 16S rRNA gene performed for six isolates with the highest activity showed that they belongs to genus Streptomyces, Actinomadura, Nonomuraea, Micromonospora. Isolate STG 15 which has the highest capability to control BLB incidenceis identified as Nonomuraea sp.]]>Ayuso-Sacido, A. and O. Genilloud,2005491024BPS.,20142014Boukaew, S. and P. Prasertsan,2014Streptomyces philanthi RM-1-138.]]>61110Technikova-Dobrova, Z., F. Damiano, S.M. Tredici, G. Vigliotta and R. di Summa et al.,2004Actinomadura sp. ATCC 39727, producer of the glycopeptide A40926: Effects of calcium ions and nitrogen sources.]]>65671677Emmert, E.A.B. and J. Handelsman,199917119Ghadin, N., N.M. Zin, V. Sabaratnam, N. Badya, D.F. Basri, H.H. Lian and N.M. Sidik,2008Streptomyces SUK 06 with antimicrobial activity from Malaysian plant.]]>7189194Gnanamanickam, S.S., V.B. Priyadarisini, N.N. Narayanan, P. Vasudevan and S. Kavitha,19997714351444Hastuti, R.D., Y. Lestari, R. Saraswati, A. Suwanto and Chaerani,2012Streptomyces spp. in controlling bacterial leaf blight disease in rice plants.]]>7217223Hopwood, D.A.,19979724652498Krishanti, N.P.R.A., A.T. Wahyudi and A.A. Nawangsih,2015Xanthomonas oryzae pv oryzae.]]>6801810Kumari, K.A., K.N.R. Kumar and C.N. Rao,20143150151Lindow, S.E. and M.T. Brandl,20036918751883Marchesi, J.R., T. Sato, A.J. Weightman, T.A. Martin, J.C. Fry, S.J. Hiom and W.G. Wade,199864795799Mew, T.W., A.M. Alvarez, J.E. Leach and J. Swings,199377512Minuto, A., D. Spadaro, A. Garibaldi and M.L. Gullino,2006Streptomyces griseoviridis and solarization.]]>25468475Mur, L.A.J., P. Kenton, A.J. Lloyd, H. Ougham and E. Prats,200859501520Nakaew, N., R. Sungthong, A. Yokota and S. Lumyong,2012Nonomuraea monospora sp. nov., an actinomycete isolated from cave soil in Thailand, and emended description of the genus Nonomuraea.]]>6230073012Navon, A.,2000Bacillus thuringiensis insecticides in crop protection-reality and prospects.]]>19669676O'Brien, R.D. and S.E. Lindow,1989Pseudomonas syringae and other bacteria.]]>79619627Saika, R.,2008pp: 111-119pp: 111-119Takahashi, Y.,2004185461Vorholt, J.A.,201210828840Wahyudi, A.T., S. Meliah and A.A. Nawangsih,2011Xanthomonas oryzae pv. oryzae bakteri penyebab hawar daun pada padi: Isolasi, karakterisasi, dan telaah mutagenesis dengan transposon.]]>158996Wang, M. and Q. Ma,2011Corynespora cassiicola.]]>331721Weller, D.M.,198826379407