Asian Journal of Plant Pathology1819-1541xxxx-xxxxScience International10.3923/ajppaj.2011.146.154Diaporthe/Phomopsis Complex Isolates in Soybean from Malaysia and Their Longevity in Stored Seeds]]>RaeisiSamiyeh PutehAdam SijamKameruzaman 4201154Diaporthe/Phomopsis Complex (DPC) can infect soybean seed and reduce its germination in the field and also survive on the seed during storage. Ten soybean seed lots which were stored at 0°C for up to 13 years were used to evaluate the percentage of DPC infection on them and also to identify the DPC isolates. Morphological and molecular methods were used to determine the longevity and frequency of DPC isolates in stored soybeans seeds. Conventional and nested Polymerase Chain Reaction (PCR) was used for amplification of pure cultures and seed lots. The longevity of isolates in storage was found to be <9 years which suggested that Diaporthe/Phomosis sp., can survive up to 9 years in cold storage (0°C). Six isolates of DPC were detected, identified and characterized based on morphological and molecular methods in soybean seeds for the first time in Malaysia. Most of the isolates identified belonged to Phomopsis longicolla and only one isolate identified from Diaporthe Phaseolorum var. sojae. All isolates that were identified using morphological technique were confirmed using molecular method and registered in national center of biotechnology information (NCBI). The result of this study showed that DPC isolates can survive for long time in storage.]]>Brown, E.A., H.C. Minor and O.H Calvert,198727895898Cui, Y.L, C.X. Duan, X.M. Wang, H.J. Li and Z.D. Zhu,2009Phomopsis longicolla causing soybean stem blight in China.]]>58799799Dellaporta, S.L., J. Wood and J.B. Hicks,198311921Dorrance, A.E. and D.R. Mills,20092009Hobbs, T.W., A.F. Schmitthenner and G.A. Kutter,1985Phomopsis species from soybean.]]>77535544Hartman, G.L., J.B. Sinclair and J.C. Rupe,19991999pp: 31-32pp: 31-32Mengistu, A., L.A. Castelbury, A.Y. Rossman, J.R. Smith and K.N. Reddy,2007Diaporth-Phomopsis from weed and their effect on soybean.]]>29283289McGee, D.C.,198670329333Pioli, R.N., E.N. Morandi, M.C. Martinez, F. Lucca, A. Tozzini, V. Bisaro and H.E. Hopp,2003Diaporthe phaseolorum variability in the core soybean-producing area of Argentina.]]>93136146Rehner, S.A. and F.A. Uecker,1994Phomopsis.]]>7216661674Roy, K.W., W.A. Miller and K.S. McLean,1994162526Roy, K.W., S. Ratnayake and K.S. McLean,1997Phomopsis longicolla.]]>19193196Sinclair, J.B. and P.A. Backman,198934th Edn.,pp: 106pp: 106Uddin, W., K.L. Backman, R.A. Pardo-Schultheiss and S.A. Rehner,1998Phomopsis isolates from peach, plum and Asian pear.]]>82732737Vrandecic, K., D. Jurkovic and J. Cosic,2006Diaporthe/phomopsis species isolated from soyabean and Abutilon theophrasti on soybean seed germination.]]>154725728Winnepenninckx, B., T. Backeljau and R. De Wachter,19939407407Yaja, J., E. Pawelzik and S. Vearasilp,20052005Zhang, A.W., G.L. Hartman, L. Riccioni, W.D. Chen, R.Z. Ma and W.L. Pedersen,1997distinguish Diaporthe phaseoluru and Phomopsis longicola from other soybean fungal pathogens and to detect them in soybean tissues.]]>8111431149Zorrilla, G., A.D. Knapp and D.C. McGee,199434172177Lori, G.A., M.N. Sisterna, M.C. Rollan and R.A. Barreyro,200149133139