American Journal of Plant Physiology1557-45391557-4547Academic Journals Inc.10.3923/ajpp.2014.16.23in vitro Regeneration and Micro Propagation
of Medicinal Sand Dune Plant Species Ipomoea Pes-caprae (L.) R. Br.]]>ThirunavukkarasuP. RamanathanT. UmamaheshwariG. ManigandanV. DineshP. 1201491Ipomoea pes-caprae is a sand dune plant commonly used as folklore medicine
for fisherman communities based on the traditional knowledge. Little information
is available on the feasibility of applying micropropagation techniques for
production of dune and marsh species. The main aim of this study is to increase
the callus induction and shoot generation of sand dune plant Ipomoea pes-caprae
with easily available low cost natural materials. Coconut water is the rich
source of carbohydrate and other nutrients which enhance callus and plant regeneration.
In our present study, we tried different type of MS medium (Full and half strength)
with coconut water at three different percentage (10, 15, 20%) and different
concentration of plant growth regulators for callus induction and shoot regeneration.
Well-developed callus inoculated in full and half strength MS medium with different
concentration of CW and plant growth regulators. The best results were accomplished
with half strength MS medium with 15% coconut water and with 2, 4-D and IAA
0.7 mg L-1 concentration which shows better callus induction and
shoot regeneration. Young shoot and root developed plants transferred to green
house and then followed to soil.]]>Agoramoorthy, G., F.A. Chen, V. Venkatesalu, D.H. Kuo and P.C. Shea,20082013111322Arti, P., R.D. Devi and U.R. Murty,1994Sorghum bicolor (L.) Moench.]]>227171Barson, M.M. and D.M. Calder,1981925565Baskaran, P. and N. Jayabalan,2005Eclipta, alba-A valuable medicinal herb.]]>41532539Desai, K.N.,19951995Faisal, M., N. Ahmad and M. Anis,2005Rauvolfia tetraphylla L. using thidiazuron.]]>80187190Gomez, K.A. and K.A. Gomez,1976Pages: 294Pages: 294Gill, R., J.M. Gerrath and P.K. Saxena,1993Pelargonium×hortorum).]]>71408413Gawde, A.J. and G.T. Paratkar,2004Eclipta alba Hassk: An approach to shorten the protocol.]]>3128132Gopalakrishnan, M., B. Janarthananm, G. Lakshmi Sai and T. Sekar,2009197987Hassan, A.S. and R. Khatun,2010Ficus glomerata Roxb., using shoot tips and nodal explants.]]>394750Hassan, A.K.M.S. and S.K. Roy,2005Gloriosa superba L. through high frequency shoot proliferation.]]>156774Hagio, T.,199444121126Li, Y., Y. Zhang, F. Feng, D. Liang, L. Cheng, F. Ma and S. Shi,2010Malus vacuolar Na+/H+ antiporter gene (MdNHX1) in apple rootstock M. 26 and its influence on salt tolerance.]]>102337345Mederos-Molina, S.,2004In vitro callus induction and plants from stem and petiole explants of Salvia canariensis L.]]>14167172Murashige, T. and F. Skoog,196215473497Mamun, M.A., M.B.H. Sikdar, D.K. Paul, M.M. Rahman and M.R. Islam,2004In vitro micropropagation of some important sugarcane varieties of Bangladesh.]]>3666669Masteller, V.J. and D.J. Holden,1970Sorghum.]]>45362364Nguyen, T.V., I.D. Godwin, J.A. Able, S.J. Gray and C. Rathus,1998in vitro of grain sorghum.]]>398890Patil, V.M. and M.S. Kuruvinashetti,1998Sorghum cultivars.]]>64217219Pelissier, B., O. Bouchefra, R. Pepin and G. Freyssinet,199094750Uno, Y., S. Nakao, Y. Yamai, R. Koyama, M. Kanechi and N. Inagaki,2009Aster tripolium L.).]]>98303309Saradamani, N., S. Muralimohan, R. Sudhakar, D. Pola and V.S.N. Dora,2003In vitro morphogenesis in cultivated varieties of Sorghum bicolor (L.) Moench.]]>44348Tripathy, S. and G. M. Reddy,2002In vitro callus induction and plantlet regeneration from Indian cotton cultivars.]]>3137142Ugraiah, A., S. Karuppusamy and T. Pullaiah,201020712Untawale, A.G. and S.A. Nair,197474151Vartak, V. and M. Shindikar,2008Bruguiera cylindrical L. towards conservation.]]>7255259De Souza, M.M., A. Madeira, C. Berti, R. Krogh, R.A. Yunes and V. Cechinel-Filho, 2000Ipomoea pes-caprae (L.) R. Br.]]>698590