American Journal of Plant Physiology1557-45391557-4547Academic Journals Inc.10.3923/ajpp.2007.90.98Pinus roxburghii (Chir Pine)]]>MalabadiRavindra B. NatarajaK. 2200722This study for the first time was aimed at developing a protocol for the genetic transformation of embryogenic tissue derived from the vegetative shoot apices of mature trees of P. roxburghii. This was achieved via the introduction of a bar-GUS cassette under the control of the ubiquitin promoter, through biolistic transfer. Expression of positive histochemical GUS activity (31%) in the bombarded embryogenic tissue was observed. PCR analysis of bar transgenes (54%) transformation efficiency indicated successful genetic modifications of P. roxburghii embryogenic tissue by the pAHC25 plasmid. This is the first successful report of genetic transformation in P. roxburghii using embryogenic tissue derived from the mature treesindicating that Chir pine is amenable to gene transfer.]]>Aronen, T.S., T.O. Nikkanen and H.M. Haggman,2003Pinus sylvestris L.) via the application of transformed pollen in controlled crossings.]]>375375378Bommineni, V.R., R.N. Chibbar, R.S.S. Datla and E.W.T. Tsang,1993Picea glauca) somatic embryos by microprojectile bombardment.]]>131723Charest, P.J., Y. Devantier and D. Lachance,1996Picea mariana (black Spruce) via particle bombardment.]]>329199Christensen, A.H. and P.H. Quail,19965213218De Block, M., J. Botterman, M. Vanderwiele, J. Dockx and C. Thoen et al.,1987625132518Ellis, D.D., D.E. McCabe, S. McInnis, R. Ramachandran and D.R. Russell et al.,1993Picea glauca by particle acceleration.]]>118489Gupta, P.K. and D.J. Durzan,19854177179Hadi, M.Z., M.D. McMullen and J.J. Finer,199615500505Haggman, H.M., T.S. Aronen and T.O. Nikkanen,199727928935Holland, L., J.E. Gemmell, J.A. Charity and C. Walter,1997Pinus radiata cotyledons by Agrobacterium tumefaciens.]]>27289304Jefferson, R.A.,19875387405Jefferson, R.A., T.A. Kavanagh and M.W. Bevan,1987639013907Jonard, R.,19861986pp: 31-48pp: 31-48Klimaszewska, K., Y. Devantier, D. Lachance, M.A. Lelu and P.J. Charest,1997Larix laricina (tamarack): Somatic embryogenesis and genetic transformation.]]>27538550Klimaszewska, K., D. Lachance, G. Pelletier, M.A. Lelu and A. Seguin,2001P. glauca, P. mariana and P. abies after cocultivation of embryogenic tissue with Agrobacterium tumefaciens. In vitro cell.]]>37748755Li, X.Q., R.C. Stahl and G. Brown,1995Agrobacterium tumefaciens.]]>4349351Malabadi, R.B. and K. Nataraja,2003Agrobacterium rhizogenes mediated transformation and bioreactor in Clitoria ternatea (Linn.).]]>4169178Malabadi, R.B., G.S. Mulgund and K. Nataraja,2005Pinus kesiya (Royle ex. Gord.) influenced by triacontanol.]]>27531537Malabadi, R.B. and J. Staden-Van,2006Pinus patula is mediated by calcium.]]>72613618Malabadi, R.B. and K. Nataraja,2006Pinus roxburghii Sarg. Trees.]]>42152159Malabadi, R.B. and K. Nataraja,2006Pinus roxburghii SARG.]]>6114120Malabadi, R.B. and K. Nataraja,2007Pinus wallichiana A.]]>24553Malabadi, R.B. and K. Nataraja,2007Pinus kesiya Royle ex. Gord. (Khasi pine).]]>68692Malabadi, R.B. and K. Nataraja,2007Pinus wallichiana A. B. Jacks (Himalayan blue pine).]]>11105111Nigro, S.A., N.P. Makunga, N.B. Jones and J. van Staden,2004Pinus patula embryonal suspensor masses.]]>44187197Parasharami, V.A., V.B. Naik, S. von Arnold, R.S. Nadgauda and D.H. Clapham,2006Pinus roxburghii sarg.).]]>24708714Peng, J., F. Wen, R. Lister and T.K. Hodges,19952791104Perl, A., H. Kless, A. Blumenthal, G. Galili and E. Galun,1992235279284Robertson, D., A.K. Weissinger, R. Ackley, S. Glover and R.R. Sederoff,1992Picea abies L. Karst) using somatic embryo explants by microprojectile bombardment.]]>19925935Tang, W., R. Sederoff and R. Whetten,2001Pinus taeda) from zygotic embryos transformed with Agrobacterium tumefaciens.]]>22115Tang, W. and R.J. Newton,200322115Tian, L.N., P.J. Charest, A. Seguin and R.G. Rutledge,2000Picea mariana).]]>19358362Vickers, J.E., G.C. Graham and R.J. Henry,19964363368Walter, C., L.J. Grace, A. Wagner, D.W.R. White and A.R. Walden et al.,1998Pinus radiata D.]]>17460468Walter, C., L.J. Grace, S.S. Donaldson, J. Moody and E. Gemmell et al.,19992915391546Wan, Y. and P.G. Lemaux,19941043748Winship, P.R.,19891712661267