American Journal of Food Technology1557-45711557-458XAcademic Journals Inc.10.3923/ajft.2013.102.113BhamaS. KarthikeyanT. RameshT. GopinathanS. 2201382The aim of the research work is to formulate a nutritional juice to fulfil the nutritional requirements of human being through cost effective manner. Thus, the developmental strategy is to produce a nutritional health drink with natural preservatives as an alternative to chemically prepared modern soft drinks to avoid health risks and increase the bioavailability of fruits and vegetables derived nutritional fractionates. Selected fruits, vegetables and some spices including indigenous species of Indian origin were utilized in a proper proportion in order to attain the human nutritional requirements. The methodology includes the extraction of nutritional juice from fruits and vegetables and subsequent analysis of nutritive value. Hence, the nutritional content, percentage of antioxidants and antimicrobial activity were also assessed in order to fulfil the nutritional requirements and longevity of the juice without any chemical additives. Upon fulfilling the criteria of nutritional requirements, the palatability was tested with many volunteers to know their willingness of consumption and found that all had accepted it. In the modern world, many people are consuming juices, foods with chemical based production or preservation without knowing the actual deteriorative and negative side effects. But the consumption of natural nutritional juice kind of products, which are produced directly from natural resources, would certainly improve the health status of individual without any negative side effects. 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