American Journal of Food Technology1557-45711557-458XAcademic Journals Inc.10.3923/ajft.2012.690.699Al-OtaibiM.M. 112012711Presently many dairy products are being used in Saudi Arabia. Monitoring and
isolation of bacterial activity is of highly significance to sustainable health
problems and issues. The objective of the study was to isolate bacteriocin-producing
Lactic Acid Bacteria (LAB) from some traditional Saudi food. A total of 50 samples
from dairy products, commonly consumed in Saudi Arabia, were screened for presence
of natural LAB. The Cell-free Supernatants (CFS) of two LAB isolates exhibited
antibacterial activities (inhibition zones >10 mm) against food-borne pathogens
(Lactococcus monocytogenes ATCC 7644, Staphylococcus aureus ATCC
29213 and Salmonella enteritidis ATCC13076). The growth inhibitory substances
of CFS were sensitive to proteases (Protenase K and Pepsin) indicating the proteinaceous
nature of inhibitors (bacteriocins) produced by the two LAB isolates. Their
bacteriocins retained activity after thermal treatments (63°C for 30 min,
100°C for 10 min or 121°C for 15 min) or at pH ranging from 4.0 to less
than 6.5. The two LAB isolates were phenotypically identified as Lactococcus
lactis subsp. lactis (camels
milk) and Lactobacillus paracasei (goats
milk). The isolated LAB (Lactococcus lactis subsp. lactis and
Lactobacillus paracasei) can be used as food preservatives and probiotics
since they inhibited well-known food-borne pathogens such as L. monocytogenes
and survived acidic conditions (pH 2.5) similar to those of the stomach.]]>Abdelgadir, W., D.S. Nielsen, S. Hamad and M. Jakobsen,2008Streptococcus infantarius subsp. infantarius.]]>127215219Alegria, A., S. Delgado, C. Roces, B. Lopez and B. Mayo,2010Lactococcus lactis strains isolated from traditional, starter-free cheeses made of raw milk.]]>1436166Ammor, S., G. Tauveron, E. Dufour and I. Chevallier,200617454461Casalta, E. and M.C. Montel,2008Lactococcus genus.]]>126271273Cheikhyoussef, A., N. Pogori, W. Chen and H. Zhang,2008125215222Chen, H. and D.G. Hoover,2003282100Cleveland, J., T.J. Montville, I.F. Nes and M.L. Chikindas,200171120De Vuyst, L. and F. Leroy,200713194199Franciosi, E., L. Settanni, A. Cavazza and E. Poznanski,200919311Gerez, C.L., M.I. Torino, G. Rollan and G.F. de Valdez,200920144148Klingberg, T., L. Axelsson, K. Naterstad, D. Elsser and B.B. Budde,2005105419431Nikolic, M., A. Terzic-Vidojevic, B. Jovcic, J. Begovic, N. Golic and L. Topisirovic,2008122162170Rivera-Espinoza, Y. and Y. Gallardo-Navarro,201027111Robinson, R.K.,20023rd Edn.,Pages: 765Pages: 765Settanni, L. and A. Corsetti,2008121123138Yateem, A., M.T. Balba, T. Al-Surrayai, B. Al-Mutairi and R. Al-Daher,20083194199