American Journal of Food Technology1557-45711557-458XAcademic Journals Inc.10.3923/ajft.2012.123.132SilvestreMarialice P.C.de SouzaMariana W.S.Lopes JuniorCarlos O.SilvaViviane D.M.AguiarMarcos J.B.AfonsoWendel O.SilvaMauro R.3201273Phenylketonuria (PKU) is a metabolic disease in which the untreated patients can
show irreversible mental retardation and the use of milk by phenylketonurics patients
is forbidden. With the aim of preparing low-phenylalanine milk hydrolysates, to
be used by phenylketonurics patients with no restriction, the Activated Carbon
(AC) was used as the adsorbent support. Also, the effects of certain parameters
were analyzed, such as enzyme type (proteases from Aspergillus sojae and
from Bacillus subtilis), temperature (30 and 50°C) and enzyme:substrate
(E:S) ratio (1:100 and 2:100). The efficiency of Phenylalanine (Phe) removal was
evaluated by second derivative spectrophotometry, measuring the Phe content of
milk as well as in its hydrolysates after AC treatment. AC showed to be an efficient
Phe adsorbent because a Phe removal from 16.28 to 58.76% was achieved, yielding
certain hydrolysates with less than 100 mg Phe 100 mL-1, that could
be used in the diet of phenylketonurics. The highest Phe removal (58.26%, in average)
was achieved using the protease from B. subtilis at 50°C and E:S ratio
of 1:100 or 2:100. At 50oC, the use of B. subtilis protease
was more efficient than A. sojae protease whereas at 30°C the trend
was inversed. The E:S ratio of 2:100 yielded greater Phe removal than 1:100 for
B. subtilis protease at 30°C and for A. sojae at 30 and 50°C.
It can be inferred that the conditions used in the study showed to be efficient
in obtaining milk with reduced Phe content, appropriate for the diet of phenylketonurics.]]>Capobiango, M., D.C.F. Lopes, R.L. Carreira, W.O. Afonso, S.D. Segall and M.P.C. Silvestre,20073119Carreira, R.L., M.R. Silva, A.L.P. Starling, M.J.B. Aguiar, J.N. Januario and M.P.C. Silvestre, 2008419Carreira, R.L., C.S. Ramos, L.A. Mundim, M.R. Silva, V.D.M. Silva and M.P.C. Silvestre,200947178Chien, H.C., L.L. Lin, S.H. Chao, C.C. Chen and W.C. Wang et al.,2002Aspergillus sojae.]]>1576119126Choi, N.S., K.T. Chang, P. Jae Maeng and S.H. Kim,2004Bacillus sp. DJ-4.]]>236325331Delvivo, F.M., C.R. Vieira, E.A.R. Biasutti, M. Capobiango, V.D.M. Silva, W.O. Afonso and M.P.C. Silvestre,2006194104Dias, D.R., D.M. Vilela, M.P.C. Silvestre and R.F. Schwan,2008Bacillus sp. isolated from coffee bean grown on cheese whey.]]>2420272034Frenhani, P.B. and R.B. Burini,199936227237Kitagawa, T., M. Owada, K. Aoki, S. Arai and T. Oura et al.,198738321327Lopes, D.C.F., F.M. Delvivo and M.P.C. Silvestre,200538447453Lopes, D.C.F., F.M. Delvivo and M.P.C. Silvestre,20063696104Lopes, D.C.F., F.M. Delvivo, J.N. Januario, M.J.B. Aguiar, A.L.P. Starling and M.P.C. Silvestre,20075191197Lopes, D.C.F., C.S. Bizzotto, R.L. Carreira, W.O. Afonso, C.O. Lopes Jr. and M.P.C. Silvestre,200865765Bajonero, L.J.L., P.L. Calderon, A.G. Mariscal, A.V. Arellano and A.L. Munguia,199156938942Mapekula, M., M. Chimonyo, C. Mapiye and K. Dzama,201124529536Morato, A.F., R.L. Carreira, R.G. Junqueira and M.P.C. Silvestre,200013843857Morais, H.A., L.M. De Marco, M.C. Oliveira and M.P.C. Silvestre,2005345969Moszczynski, P. and J. Idziac,199329302306Rao, M.B., A.M. Tanksale, M.S. Ghatge and V.V. Deshpande,199862597635Reed, G.,19752nd Edn.,Pages: 573Pages: 573Silva, V.D.M., L.M. De Marco, W.O. Afonso, D.C.F. Lopes and J.N. Januario et al.,20072327341Silva, M.C., V.D.M. Silva, T.F. Fernandes and M.P.C. Silvestre,2010352946Silvestre, M.P.C., C.R. Vieira, M.R. Silva, M.C. Silva, C.O. Lopes Jr. and V.D.M. Silva,20095111Silvestre, M.P.C., C.O. Lopes, M.R. Silva, V.D.M. Silva, M.J.B. Aguiar and L.L. Amorin,20116893903Silvestre, M.P.C., M.C. Silva, V.D.M, Silva, M.R Silva and L.L. Amorin,20114302314Sitta, A., A.G. Barschak, M. Deon, A.T. Barden and G.B. Biancini et al.,200927243247Soares, R.D.L., E.A.R. Biasutti, M. Capobiango, C.R. Vieira and V.D.M. Silva et al.,200625325332Soares, R.D.L., M. Capobiango, E.A.R. Biasutti and M.P.C. Silvestre,2007214556Urano, T., H. Ihara, K. Umenara, Y. Suzuki and M. Oike et al.,20012762469024696Wasserstein, M.P., S.E. Snyderman, C. Sansaricq and M.S. Buchsbaum,200687272277Yang, Y., L. Jiang, L. Zhu, Y. Wu and S. Yang,200081113118