American Journal of Food Technology1557-45711557-458XAcademic Journals Inc.10.3923/ajft.2009.226.240KanuPhilip JohnKanuJestina Baby H. SandyEdward B.A. KandehJoseph MornyaPhilip M.P. HuimingZhou 6200946Sesame Protein Hydrolysate (SPH) was prepared from defatted
sesame (Sesamum indicum L.) flour (DSF) after screening with different
proteases. The proteases under different conditions showed varied effects
on the protein recovery process. For example, Alcalase®
2.4 L produced the highest degree of protein recovery (96.68%) at 60°C
and pH 8 followed by Flavourzyme (69.76%). However, at 50°C and pH
7, the highest protein recovery was noted for Flavourzyme (79.28%) followed
by Alcalase 2.4 L (77.62%). The hydrolysis conditions (Temperature T,
pH, Enzyme/Substrate E/S, time t) were engineered to optimize the degree
of hydrolysis (DH) with the process studied using the Response Surface
Methodology (RSM). The DH ranged from 1.19 to 18.8% while the solubility
of the Defatted Sesame Protein Isolate (DSPI) increased with increase
in pH. The SPH was observed to be a better emulsifier with significantly
higher foaming properties, water and oil capacities compared to the untreated
DSF. Nonetheless, the stability of the resulting foam diminished on standing
over time. The Sesame protein hydrolysate obtained using Alcalase was
noted to have better functional attributes compared to that obtained using
Flavourzyme.]]>Adler-Nissen, J.,1986pp: 116-124pp: 116-124Abou-Gharbia, H.A., A.A.Y. Shehata and F. Shahidi,200033331340Bandyopadhyay, K. and S. Ghosh,2002Sesamum indicum L.) protein isolates.]]>5068546857Barbut, S.,19991999pp: 186-225pp: 186-225Bernard-Don, L.S., A.M.R. Pilosof and G.B. Bartholomai, 199168102105Box, G.E.P. and D.W. Behnken, 1960 2455475Cheison, S.C., Z. Wang and S. Xu,20078011341145Chen, E.C.F., S.S.K. Tai, C.C. Peng and J.T.C. Tzen,199839935941Chiang, W.D., C.J. Shih and Y.H. Chu,199965189194Chove, B.E., A.S. Grandison and M.J. Lewis,200282267272Claver, I.P. and Z. Huiming,2005291326Coulman, K.D., Z. Liu, W.Q. Hum, J. Michaelides and L.U. Thompson,200552156165Darwicz, M., J. Dziuba and P.W. Caessens,2000938Diniz, F.M. and A.M. Martin, 1996Squalus acanthias) muscle. ]]>31419426Ferreira., I.M.P.L.V.O., O. Pinho, M.V. Mota, P. Tavares and A. Pereira et al.,2007 17 481487Fuhrmeister, H. and F. Meuser,200356119129Gonzalez-Perez, S., J.M. Vereijken, G.A.Van-Koningsveld and H. Gruppen,2005Helianthus annuus) proteins.]]>5364696476Hrckova, M., M. Rusnakova and J. Zemanovic,200220714James, C.S.,19951st Edn.,Pages: 178Pages: 178Jung, S., P.A. Murphy and L.A. Johnson,200570C180C187Kanu, P.J., H.M. Zhou, K.J. Kanu, K.X. Zhu, K.R. Zhu and H. Qian,2007Sesamum indicum L.) protein extractability with water and the analysis of the protein extracted for it amino acid profile.]]>6447455Khalida, E.K., E.E. Babikerb and A.H. El Tinay,200382361366Mahmoud, M.I., 19944889113Mahmoud, M.I., W.T. Malone and C.T. Cordle, 1992 57 1223 1223Martin, A.H., K. Grolle, M.A. Bos, M.A. Cohen-Stuart and T. van Vilet, 2002254 175183Mota, M.V.T., I.M.P.L.V.O. Ferreira, M.B.P. Oliveira, C. Rocha, J.A. Teixeira, D. Torres and M.P. Goncalves,20042726252639Mutilangi, W.A.M., D. Panyam and A. Kilara, 1995 511041109Nilo-Rivas, R., E. Jane, P. Dench and C. Caygill,1981Sesame indicum L.) seed and the preparation of two proteins isolate.]]>32565571Radha, C., P.R. Kumar and V. Prakash,200710611661174SAS,2002Version 9.,Shahidi, F., C.M. Liyana-Pathirana and D.S. Wall,200699478483Sinha, R., C. Radha, J. Prakash and P. Kaul,200710114841491Taha, F.S. and M.A. Ibrahim,200253273281Tsumura, K., T. Saito, K. Tsugea, H. Ashida, W. Kugimiya and K. Inouye, 200538 255261Xiangzhen, K., Z. Huiming and Q. Haifeng, 200701 615620