American Journal of Drug Discovery and Development2150-427x2150-4296Academic Journals Inc.10.3923/ajdd.2014.90.101Syzygium
polyanthum Leaves]]>Sulistiyani FalahS. WahyuniW.T. SugaharaT. TachibanaS. Syaefudin 2201442The research objective is to obtain the cellular mechanism(s) of Syzygium
polyanthum leaves for its development into commercial herbal product for
certain degenerative diseases. The approach was to conduct activity-guided fractionation
of metabolite extracts based on the antioxidant and the apoptotic modulation
activities using cultured cells. Antioxidant activity of the active fractions
were screened using 1,1-diphenyl-2-picrylhydrazil (DPPH) free radical assay
and thiobarbituric acid (TBA) assay. The cellular effects were investigated
using cultures of HB4C5 human hybridoma and mouse colon 26 adenocarcinoma cells.
The flavonoids extract which has the lowest IC50 value of 14.84 μg
mL-1 for DPPH assay among other metabolites, was found to have significant
cytotoxic effect on both HB4C5 and colon 26 cells. Specific assays for apoptosis
using caspase 3-gene expression and annexin-V flow cytometry analyses, supported
the effect of cell proliferation inhibition which was not due to apoptosis.
Subsequent cell cycle analysis indicated that crude extract may induce cell
cycle arrest of HB4C5 cells at G1 to S phase, whereas active compound fractions
of flavonoids stacked the cell cycle at G2/M phase. These observations suggest
that flavonoid extracts of Syzygium polyanthum posses active compound(s)
with anti-proliferative effect. This is a new finding for therapeutic potential
of Syzygium polyanthum leaves in Indonesian traditional medicines.]]>Andree, H.A., C.P. Reutelingsperger, R. Hauptmann, H.C. Hemker, W.T. Hermens and G.M. Willems,19902549234928Cheng, J., H. Imanishi, Y. Amuro and T. Hada,200299755761Harborne, J.B.,19983rd Edn.,Pages: 302Pages: 302Ismail, A., M. Mohamed, S.A. Sulaiman and W.A.N.W. Ahmad,2013Syzygium polyanthum (Wight) Walp. var. polyanthum leaves in anaesthetized rats.]]>2013Kikuzaki, H. and N. Nakatani,19935814071410Kusuma, I.W., H. Kuspradini, E.T. Arung, F. Aryani, Y.H. Min, J.S. Kim and Y.U. Kim,2011Murraya koenigii, Syzygium polyanthum and Zingiber purpurea.]]>47579Lelono, R.A.A., S. Tachibana and K. Itoh,2009In vitro antioxidative activities and polyphenol content of Eugenia polyantha wight grown in indonesia.]]>1215641570Li, J., H.Y. Cheung, Z. Zhang, G.K.L. Chan and W.F. Fong,20075683144Mabry, T.J., K.R. Markham and M.B. Thomas,1970Pages: 354Pages: 354Nishimoto, S., K. Kawane, R. Watanabe-Fukunaga, H. Fukuyama and Y. Ohsawa et al.,200324210711074Permana, D., N.H. Lajis, K. Shaari, A.M. Ali and M.M. Mackeen et al.,2003Garcinia atroviridis Griff ex T. Anders (Guttiferae).]]>58b332335Perumal, S., R. Mahmud, S.P. Piaru, L.W. Cai and S. Ramanathan,2012Ziziphus mauritiana and Syzygium polyanthum.]]>8535541Ramos, S.,200718427442Salazar-Aranda, R., L.A. Perez-Lopez, J. Lopez-Arroyo, B.A. Alanis-Garza and N.W. de Torres,20112011Schutte, B., R. Nuydens, H. Geerts and F. Ramaekers,1998866369Scicchitano, P., M. Cameli, M. Maiello, P.A. Modesti and M.L. Muiesan et al.,201461132Sugahara, T., M. Ueno, Y. Goto, R. Shiraishi, M. Doi, K. Akiyama and S. Yamauchi,20067021312137Sugahara, T., S. Yano and T. Sasaki,200367393395Tabas, I.,20052522552264Tominaga, T., M. Ishiyama, F. Ohseto, K. Sasamoto, K. Hamamoto, K. Suzuki and M. Watanabe,1999364750Wong, S.P., L.P. Leong and J.H.W. Koh,200699775783Yamaguchi, T., H. Takamura, T. Matoba and J. Terao,19986212011204