Asian Journal of Crop Science1994-78792077-2041Asian Network for Scientific Information10.3923/ajcs.2014.58.66Peperomia obtusifolia]]>El-NaggarHany M. R. OsmanAmira 1201461Peperomia obtusifolia is a bushy upright species belong to the Piperaceae,
native to Florida, Mexico and the Caribbean, as an alternative to the conventional
vegetative propagation methods is becoming an important way to increase shoot
proliferation rates and produce of new varieties through transformation; therefore,
the purpose of this study was to establish a protocol for in vitro production
of Peperomia obtusifolia and to compare between two explants for shoot
proliferation and organogenesis. Single node stem segments and leaf segments
of Peperomia obtusifolia were cultured on solidified Murashige and Skoog
(MS) basal medium supplemented with kinetin (Kin) at 0, 2.5, 5 and 10 mg L-1
and naphthalene acetic acid (NAA) at 0, 1, 2.5 and 5 mg L-1. All
explants generated from leaf segments turned dark brown and died no shoots or
callus were formed. Percentage of successful shoot regenerated from single node
stem segments increased by increasing kinetin concentration then declined at
the highest concentration of kinetin (10 mg L-1) while different
concentrations of NAA almost had no effect on percentage of shoot formation.
Kinetin at 5 mg L-1 combined with NAA at 1 mg L-1 was
the most effective concentrations in percentage of shoot proliferation, number
of shoots, fresh weight, total leaf area, total chlorophyll and callus weight
on the other hand this concentration caused a reduction in shoot length and
a delay in days to proliferation.]]>Ahmadabadi, M. and R. Bock,2010Peperomia species.]]>33329334Anderson, J.W. and K.S. Rowan,19681968pp: 1447-1455pp: 1447-1455Angelova, Y., S. Petkova, E. Zozikova, E. Kotseva and L. Iliev,2001273642Campanoni, P. and P. Nick,2005137939948Chory, J., D. Reinecke, S. Sim, T. Washburn and M. Brenner,1994104339347Han, B.H., H.J. Yu, B.W. Yae and K.Y. Peak,2004In vitro micropropagation of Lilium longiflorum Georgia by shoot formation as influenced by addition of liquid medium.]]>1033949Holthe, P.A., L.D.S. Sternberg and I.P. Ting,1987Peperomia scandens.]]>84743747Hui, L.H. and S.Y. Zee,1981In vitro propagation of Peperomia viridis using medium supplemented with ginseng powder.]]>168687Robert, J. and C. Louis,2012in vitro plant regeneration of Dipteracanthus prostratus (Poir.) Nees.-a medicinal herb.]]>2S484S487Juvany, M., M. Muller and S. Munne-Bosch,2012871018Bacchetta, L., P.C. Remotti, C. Bernardini and F. Saccardo,2003Lilium.]]>743744Lou, H., P. Chen, H. Zheng, C. Xu and H. Lu,2012715771583Mik, V., L. Szucovs, M. Smehilovs, M. Zatloukal and K. Dolezal et al.,201172821831Mok, D.W.S. and M.C. Mok,20015289118Muhammad, A., H. Rashid, I. Hussain and S.M.S. Naqvi,2007Musa spp. AAA Group) Basrai.]]>4212531255Mungole, A., R. Awati, S. Dey, A. Chaturvedi and P. Zanwar,2009In-vitro callus induction and shoot regeneration in Ipomoea obscura (L.): Potent Indian medicinal plant.]]>22426Murashige, T. and F. Skoog,196215473497Negahdar, N., B. Kaviani, D. Hashemabadi and M. Zarchini,2012Robinia pseudoacacia L.).]]>347974800Nissen, S.J. and E.G. Sutter,199025800802Nitsch, J.P., C. Nitsch, L.M.E. Rossini and D.B.D. Ha,196717446453Pan, B., Y.M. Bai, S. Leibovitch and D.L. Smith,200011179186Rana, M.S., J.M.S. Rawat and S.S. Rawat,2011Brassica oleracea var. italica).]]>3282285SAS,2002Version 9.0.38,Ishimori, T., Y. Niimi and D.S. Han,2007Lilium x formolongi White Aga cultured in vitro.]]>88313318Tounekti, T., I. Hernandez, M. Muller, H. Khemira and S. Munne-Bosch,2011Salvia officinalis.]]>4911651176Tukey, J.W.,1994Pages: 469Pages: 469Von Abrams, G.J. and H.K. Pratt,19664115251530Wanke, S., M.S. Samain, L. Vanderschaeve, G. Mathieu, P. Goetghebeur and C. Neinhuis,2006Peperomia (Piperaceae) inferred from the trnK/matK region (cpDNA).]]>893102Werner, T., V. Motyka, M. Strnad and T. Schmulling,2001981048710492Zhang, K., D.S. Letham and P.C. John,1996cdc2-like H1 histone kinase.]]>200212