Asian Journal of Biological Sciences1996-3351xxxx-xxxxScience International10.3923/ajbs.2019.557.564Combretum dolichopentalum Exhibits Broad-spectrum Antimicrobial Effect on Nosocomial Organisms]]>Favour NtiteUjowundu Nathan NdudiOparaeche Cosmas OnyekachiUjowundu Ikenna NdubuisiNwachukwu Afoma MariaNwobodo Okokon UdohEnomfon 32019123Background and Objective: The emergence of resistant strains of bacteria and fungi has resulted in an increase in nosocomial infections which has become a worldwide problem in clinical medicine. This study investigated an alternative source of potent antimicrobial agents. It provided evidence that embedded in Combretum dolichopentalum are phyto-active constituents capable of exhibiting broad-spectrum antimicrobial effect on Gram-positive and Gram-negative organisms, the two major disease-causing bacteria groups not excluding fungi. Materials and Methods: Isolates of Escherichia coli, Streptococcus pneumonia, Staphylococcus aureaus, Klebsiella pneumoniae, Pseudomonas aeroginosa and Candida albicans were tested against methanol extract of C. dolichopentalum using 2, 3, 5-triphenyltetrazolium chloride (TTC) to assay total dehydrogenase activity (DHA) which indicates the viability of microorganisms in the absence of antibiotics. Results: Methanol extract of C. dolichopentalum dose dependently inhibited 50 and 80% of the isolates at concentrations of 503.54 and 1801.41 μg mL1 for E. coli, 253.12 and 1077.24 μg mL1 for S. aureaus, 732.5954 and 3047.6054 μg mL1 for S. pneumonia,681.8154 and 2234.3454 μg mL1 for K. pneumoniae, 111.8654 and 554.9954 μg mL1 for P. aeroginosa and 171.88 and 454.33 μg mL1 for C. albicans, respectively. 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