Asian Journal of Biological Sciences1996-3351xxxx-xxxxScience International10.3923/ajbs.2019.356.363Escherichia coli from Clinical and Environmental Samples in Ghana]]>AyefoumiAdinortey Cynthia Damian H.A.K.Amewowor Isaac K.A.Galyuon PlasOtwe Emmanuel Daniel K.A.Asante LeviYafetto BuenorAdinortey Michael 22019122Background and Objective: Throughout the world, antibiotic resistance has been recognized as an emerging major issue of concern. In this study, genes responsible for the production of extended spectrum beta-lactamase (ESBL) in various E. coli isolates were characterized. Materials and Methods: Isolates of E. coli were obtained from clinical and environmental samples. These samples were collected from Cape Coast, Ghana. The isolates were tested for ESBL production. Subsequently, PCR assays of DNA extractions from the isolates were used to determine prevalence of blaTEM and blaSHV genes. Results: Findings revealed that more ESBL producers were phenotypically identified in E. coli isolates recovered from clinical than those recovered from environmental samples. Very low percentages of environmental and clinical isolates that were phenotypically confirmed as non-ESBL producers were found to carry only blaTEM. There was no detection of either gene in majority of the environmental and clinical isolates that were phenotypically confirmed as non-ESBL producers. Conclusion: The environment could be a repository of blaTEM and blaSHV genes thus it is imperative to intensify public health education on the risks associated with the misuse of antibiotics.]]>Motta, R.N., M.M. Oliveira, P.S.F. Magalhaes, A.M. Dias, L.P. Aragao, A.C. Forti and C.B.M. Carvalho,2003Enterobacteriaceae isolated from diabetes foot infections in a Brazilian diabetic center.]]>7129134CDC.,20132013Livermore, D.M.,200336S11S23Bradford, P.A.,200114933951Pitout, J.D.D. and K.B. Laupland,20088159166Wiegand, I., H.K. Geiss, D. Mack, E. Sturenburg and H. Seifert,2007Enterobacteriaceae by use of semiautomated microbiology systems and manual detection procedures.]]>4511671174Feglo, P., Y. Adu-Sarkodie, L. Ayisi, R. Jain and R.R. Spurbeck et al.,2012Escherichia coli in Kumasi, Ghana.]]>51728730Obeng-Nkrumah, N., K. Twum-Danso, K.A. Krogfelt and M.J. Newman,201389960964Ayisi, L.A. and Y. Adu-Sarkodie,2015Escherichia coli and Klebsiella species in Kumasi, Ghana.]]>58186Oduro-Mensah, D., N. Obeng-Nkrumah, E.Y. Bonney, E. Oduro-Mensah, K. Twum-Danso, Y.D. Osei and S.T. Sackey,2016Enterobacteriaceae in a tertiary hospital in Ghana.]]>2016Sorlozano, A., J. Gutierrez, F. Fernandez, M.J. Soto and G. Piedrola,2004Escherichia coli in Granada (Spain).]]>5499104Rasmussen, M.M., J.A. Opintan, N. Frimodt-Moller and B. Styrishave,2015Escherichia coli isolates in imported and locally produced chicken meat from Ghana.]]>2015Corkish, J.D. and R.K. Assoku,1973E. coli and Salmonella) isolates from poultry in Ghana.]]>12430432Sackey, B.A., P. Mensah, E. Collison and E. Sakyi-Dawson,2001Campylobacter, Salmonella, Shigella and Escherichia coli in live and dressed poultry from metropolitan Accra.]]>712128Donkor, E.S and E.N. Nyarko,20082008Donkor, E., T. Nortey, J. Opintan, N. Dayie and M. Akyeh,2007Salmonella typhi and Staphylococcus aureus isolates and the effect of some media on susceptibility testing results.]]>2007Adinortey, C.A., D.H.A.K. Amewowor, E.P. Otwe, I.K.A. Galyuon and D.K.A. Asante,2017Escherichia coli isolated from clinical and environmental samples in Cape Coast, Ghana.]]>12170176Newman, M.J., E. Frimpong, E.S. Donkor, J.A. Opintan and A. Asamoah-Adu,201149215220Ghana Health Service,20082008CLSI.,20102010Lane, D.J.,19911991pp: 115-175pp: 115-175Zhang, C.H., X.G. Zhang, Y.S. Shen and J.H. Wang,20111010321036Paterson, D.L. and R.A. Bonomo,200518657686Wellington, E.M.H., A.B.A. Boxall, P. Cross, E.J. Feil and W.H. Gaze et al.,201313155165Bush, K. and G.A. Jacoby,201054969976Woodford, N. and A. Sundsfjord,200556259261Maningi, N.E., M.M. Ehlers, A.A. Hoosen, E. Makgothlo, S.V. Omar and M.M. Kock,2010Klebsiella pneumonia isolates in Pretoria.]]>2010Wolny-Koladka, K. and A. Lenart-Boron,2016Escherichia coli.]]>2016Yazdi, M., A. Nazemi, M. Mirinargasi, M. Jafarpour and S. H. Sharifi,2012Escherichia coli.]]>324542458