Asian Journal of Biological Sciences1996-3351xxxx-xxxxScience International10.3923/ajbs.2019.148.155Streptomyces sp. BO07 in LPS-induced RAW 264.7 Cells]]>TaechowisanThongchai ChaiseangSuchanya PuckdeeWinyou PhutdhawongWaya S. 22019122Background and Objective: Two new biphenyls, 3'-hydroxy-5-methoxy-3,4-methylenedioxybiphenyl (1) And 3'-hydroxy-5,5'-dimethoxy-3,4-methylenedioxybiphenyl (2) Have been isolated from Streptomyces sp. BO07, an endophyte in the root tissue of Boesenbergia rotunda (L.) Mansf A. Although, there are many biphenyls on anti-inflammatory activity have been reported. This study investigated the anti-inflammatory property of these biphenyls on LPS-induced macrophage RAW 264.7 cells. Materials and Methods: The effects of the two biphenyl compounds were investigated on the formation of NO, PGE2, TNF-α, IL-1β, IL-6, IL-10 and also on inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2) in LPS-induced murine macrophage RAW 264.7 cells. Results: The data obtained were consistent with the modulation of TNF-α, IL-1β, IL-6, IL-10 production by these biphenyls at concentration of 5-20 μg mL1. A similar effect was also observed when LPS-induced NO release, iNOS and COX-2 enzyme expression were tested. The inhibitory effects were shown in concentration-dependent manners. However these compounds did not significantly reduce the formation of PGE2. Conclusion: From the obtained results, it is concluded that these biphenyls possess anti-inflammatory activity on LPS-induced RAW 264.7 cells.]]>Pablos, J.L. and J.D. Canete,201313705711Dos Santos, G., M.A. Kutuzov and K.M. Ridge,2012303L627L633Elinav, E., R. Nowarski, C.A. Thaiss, B. Hu, C. Jin and R.A. Flavell,201313759771Scharl, M. and G. Rogler,2012301219Ali, R.A.R. and L.J. Egan,20111214241432Rossol, M., H. Heine, U. Meusch, D. Quandt, C. Klein, M.J. Sweet and S. Hauschildt,201131379446Lawrence, T., D.A. Willoughby and D.W. Gilroy,20022787795Taechowisan, T., S. Chaisaeng and W.S. Phutdhawong,2017Streptomyces sp. BO-07: An endophyte in Boesenbergia rotunda (L.) Mansf A.]]>2813301346Tiwari, B.K., V.P. Valdramidis, C.P. O'Donnel, K. Muthukumarappan, P. Bourke and P.J. Cullen,20095759876000Chun, H.K., Y. Ohnishi, K. Shindo, N. Misawa, K. Furukawa and S. Horinouchi,2003Streptomyces lividans cells carrying shuffled biphenyl dioxygenase genes.]]>21113121Furukawa, K., H. Suenaga and M. Goto,200418651895196Benavente-Garcia, O., J. Castillo, J. Lorente, A. Ortuno and J.A. Del Rio,2000Olea europaea L. leaves.]]>68457462Manach, C., A. Mazur and A. Scalbert,2005167784Middleton, Jr. E., C. Kandaswami and T.C. Theoharides,200052673751Puupponen-Pimia, R., L. Nohynek, C. Meier, M. Kahkonen, M. Heinonen, A. Hopia and K.M. Oksman-Caldentey,200190494507Samman, S., P.M.W. Lyons and N.C. Cook,19981998pp: 469-482pp: 469-482Deep, A., S. Jain and P.C. Sharma,2010676367Kuo, W.L., C.Y. Chung, T.L. Hwang and J.J. Chen,2013Magnolia officinalis and their anti-inflammatory activities.]]>85153160Chung, C.Y., W.L. Kuo, T.L. Hwang, M.I. Chung and J.J. Chen,2015Magnolia denudata and their anti‐inflammatory activity.]]>1212631270Lin, C.H., H.S. Chang, C.H. Liao, T.H. Ou, I.S. Chen and I.L. Tsai,2010Rhaphiolepis indica.]]>7316281631Green, L.C., D.A. Wagner, J. Glogowski, P.L. Skipper, J.S. Wishnok and S.R. Tannenbaum,198215N] nitrate in biological fluids.]]>126131138Taechowisan, T., A. Wanbanjob, P. Tuntiwachwuttikul and J. Liu,2009Streptomyces sp. SUC1 in LPS-induced RAW 264.7 cells.]]>206777Watkins, L.R., S.F. Maier and L.E. Goehler,199563289302Masferrer, J.L., B.S. Zweifel, P.T. Manning, S.D. Hauser and K.M. Leahy et al.,1994in vivo is anti-inflammatory and nonulcerogenic.]]>9132283232Moncada, S. and A. Higgs,199332920022012Medzhitov, R.,2008454428435Cook, J. and T. Hagemann,201313595601Laveti, D., M. Kumar, R. Hemalatha, R. Sistla and V.G.M. Naidu et al.,201312349361Alexander, C. and E.T. Rietschel,20017167202Süleyman, H., B. Demircan and Y. Karagoz,200759247258Seibert, K., Y. Zhang, K. Leahy, S. Hauser and J. Masferrer et al.,1994911201312017Murakami, A. and H. Ohigashi,200712123572363Weisz, A., L. Cicatiello and H. Esumi,1996G-monomethyl-L-arginine.]]>316209215Phipps, R.P., S.H. Stein and R.L. Roper,199112349352Gao, L., W.E. Zackert, J.J. Hasford, M.E. Danekis and G.L. Milne et al.,20032782847928489Chang, H.P., S.Y. Huang and Y.H. Chen,20055325302534Feldmann, M., F.M. Brennan and R.N. Maini,199614397440Hasko, G., Z.H. Nemeth, C. Szabo, G. Zsilla, A.L. Salzman and E.S. Vizi,199845183187