Asian Journal of Biological Sciences1996-3351xxxx-xxxxScience International10.3923/ajbs.2019.114.121Trichoderma, Saccharomyces and Rhizobium Metabolites Against Damping-off and Root Rot Pathogens in Peanut Plant]]>El-HaiK.M. Abd AliAbeer A. 22019122Background and Objective: In Egypt, peanut is one of the most important crops for exportation and local consumption. It is a good source of dietary proteins and contains high lipid that is rich in unsaturated fatty acids. Damping-off and root rot diseases are the main constraints in peanut production. The current study aimed to determine the effect of cell-free filtrates of Trichoderma harzianum (T), Saccharomyces cerevisiae (Y) and/or Rhizobium japonicum (R) on the control of damping-off and root rot diseases in peanut. Materials and Methods:Fusarium solani, Rhizoctonia solani and Sclerotium rolfsii were the common causal pathogens isolated from naturally infected peanut, causing damping-off and root rot diseases. Peanut seeds cv. Giza 6 were soaked for 4 h in each tested treatment before sowing; treatments also used as a foliar spraying at 28 days from sowing. While, fungicide Rizolex-T50 was used as seed coating at 3 g kg1 seeds. Results: In the in vitro tests, the combined application of T+Y+R culture filtrates at the rate of 1:2:1 and the dual application of T+Y completely inhibited the mycelial growth of the pathogenic fungi. While, R alone had no significant effect on the fungal linear growth. In vivo, the best protection against damping-off and root rot diseases were observed under fungicide (Rizolex-T50) application followed by the combination of T+Y+R (1:2:1). All culture filtrates of T, Y, R and their combinations stimulated the activity of polyphenol oxidase, peroxidase and chitinase. The highest activity was obtained by the combined application of T+Y+R (2:1:1) for polyphenol oxidase and peroxidase. While the highest chitinase activity was observed with Y treatment alone. The application of T+Y+R increased the percentage of plant survivals and improved plant growth and yield. Conclusion: The application of a mixture of biotic culture filtrates was more effective in decreasing the infection of soil borne fungi in peanut. Wherein, a commercial production of a mixture of growth filtrates of T+Y+R is recommended for therapy both damping-off and root rot diseases in peanut plant.]]>Higgs, J.,2003335664Chukwumah, Y., L. Walker, B. Vogler and M. Verghese,20075592669273Mahmoud, E.Y., H.M. El. El-Badawy and M.M.A. Khaleifa,2008Rhizobium and VA-mycorrhizae on peanut root rot diseases under different sources of phosphorus fertilizer.]]>465161Metwally, A.H., E.Y. Mahmoud, S.Y.M. Shokry and Z.N. Hussin,20063135373548Vinale, F., K. Sivasithamparam, E.L. Ghisalberti, R. Marra, S.L. Woo and M. Lorito,2008Trichoderma-plant-pathogen interactions.]]>40110Gnanamanickam, S.S.,2002Pages: 480Pages: 480Abou-Zeid, N.M., M.K. Arafa and S. Attia,20038114911503Elwakil, M.A., O.A. Awadallah, I.M. El-Refai, M.A. El-Metwally and M.S. Mohammed,20098133143De Jensen, C.E., J.A. Percich and P.H. Graham,2002Bacillus subtilis and Rhizobium inoculation of dry bean seed on root rot severity and yield in Minnesota.]]>459899Chakrabarty, U., B. Sarkar and B.N. Chakraborty,2003Bradyrhizobium japonicum and Trichoderma harzianum, associated changes in enzyme activities and phytoalexin production.]]>332125Booth, C.,1977Pages: 237Pages: 237Sneh, B., L. Burpee and A. Ogoshi,1992Rhizoctonia Species.]]>Pages: 133Pages: 133Sime, A.D., L.L. Abbott and S.P. Abbott,20029410871088Somasegaran, P. and H.J. Hoben,1985Pages: 367Pages: 367Esterbauer, H., E. Schwarzl and M. Hayn,197777486494Kato, M. and S. Shimizu,198765729735Monreal, J. and E.T. Reese,1969Serratia marcescens.]]>15689696Shakeri, J. and H.A. Foster,2007Trichoderma harzianum in relation to pathogenicity to insects.]]>40961968Reino, J.L., R.F. Guerrero, R. Hernandez-Galan and I.G. Collado,2008Trichoderma.]]>789123Barakat, F.M., K.A. Abada, N.M. Abou-Zeid and Y.H.E. El-Gammal,2013Trichoderma spp. on Botrytis fabae the causative agent of faba bean chocolate spot.]]>21118Prasad, B.N. and M.R. Kumar,2011Trichoderma spp. On growth and sclerotial viability of Rhizoctonia solani, incitant of sheath blight of rice.]]>13742Saber, W.I.A., K.M. Abd El-Hai and K.M. Ghoneem,2009Trichoderma and Rhizobium on both biocontrol of chocolate spot disease and induction of nodulation, physiological activities and productivity of Vicia faba.]]>4286300Harman, G.E., C.R. Howell, A. Viterbo, I. Chet and M. Lorito,2004Trichoderma species-opportunistic, avirulent plant symbionts.]]>24356Li, L. and J.C. Steffens,2002215239247Raj, S.N., B.R. Sarosh and H.S. Shetty,200633563571Caruso, C., G. Chilosi, L. Leonard, L. Bertin, P. Magro, V. Buonocore and C. Caporale,200158743750Abd-El-Khair, H., R.K.M. Khalifa and K.H.E. Haggag,2010Trichoderma species on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants.]]>6486497Hassanein, N.M., A.A. El-Mehalawy, H.M. Khater, A.K. El-Zahraa and A.Y. Youssef,2002Rhizosphere actinomycetes and yeast fungi for the biological control of late wilt disease of maize caused by Cephalosporium maydis.]]>1167188Santos, A., A. Sanchez and D. Marquina,2004Botrytis cinerea.]]>159331338El-Saidy, A.E.A. and K.M. Abd El-Hai,201146481Urquhart, E.J. and Z.K. Penja,2002Tilletiopsis species, phyllosphere yeasts that are antagonists of powdery mildew fungi.]]>48219229Ahmad, F., I. Ahmad and M.S. Khan,2008168173181Chowdhury, A.K.,200325355359Wang, Y.Y., Y.X. Duan and L.J. Chen,2008Rhizobium against pathogens of soybean root.]]>38706712Kloepper, J.W., G. Wei and S. Tuzun,1992Colletotrichum orbiculare.]]>1992pp: 185-191pp: 185-191