Asian Journal of Biological Sciences1996-3351xxxx-xxxxScience International10.3923/ajbs.2017.72.79Morus alba Leaves in Hela Cell Line]]>FallahSoudabeh HajihassanZahra SadeghiAsie 22017102Background and Objective:Morus alba leaves is a natural source of many compounds with different biological effects. It has been described to possess anti-inflammatory, antioxidant and cytotoxic activities. The aim of this study was to evaluate the cytotoxicity of total flavonoid extract from Morus alba (MTF) leaves and to determine its effect on viability of Hela cells. Materials and Methods: The MTT assay was used for quantitative and rapid measurement of Hela cancer cells viability. Apoptotic effect of MTF on Hela cells was also determined via DNA fragmentation analysis by diphenylamine assay and agarose gel electrophoresis. Also in order to determine if cytochrome c involves in apoptosis induced by flavonoid extract of Morus alba leaves, quantitative real-time PCR and western blotting were done. All statistical tests were performed using the SPSS statistical software and significant differences (p<0.05) were determined by one way ANOVA. Results: A strong and dose-dependent inhibition of cancer cell viability after treatment with MTF extract was achieved. DNA fragmentation was significantly observed at a concentration of 1.5 mg mL1 of MTF when compared with controls. Cytochrome C release from mitochondria into cytosol was observed after treatment of Hela cells with MTF indicating apoptosis. Conclusion: These results demonstrated that MTF extract has cytotoxic and apoptotic activities on Hela cells and it can be used as an anticancer drug.]]>Datwyler, S.L. and G.D. Weiblen,200491767777Wei, H., J.J. Zhu, X.Q. Liu, W.H. Feng, Z.M. Wang and L.H. Yan,20162016Chen, Y.C., Y.J. Tien, C.H. Chen, F.N. Beltran and E.C. Amor et al.,2013Morus alba and active compound oxyresveratrol exert anti-inflammatory activity via inhibition of leukocyte migration involving MEK/ERK signaling.]]>2013Jeong, J.C., S.W. Jang, T.H. Kim, C.H. Kwon and Y.K. Kim,2010Moris fructus) extracts induce human glioma cell death in vitro through ROS-dependent mitochondrial pathway and inhibits glioma tumor growth in vivo.]]>62402412Eo, H.J., J.H. Park, G.H. Park, M.H. Lee, J.R. Lee, J.S. Koo and J.B. Jeong,2014Morus alba L.) root bark.]]>2014Park, S., Y.S. Kim, H.A. Lee, Y. Lim and Y. Kim,201377722728Nam, S.Y., H.K. Yi, J.C. Lee, J.C. Kim and C.H. Song et al.,200225191196Kikuchi, T., M. Nihei, H. Nagai, H. Fukushi, K. Tabata, T. Suzuki and T. Akihisa,2010Morus alba L. induces apoptotic cell death in HL60 human leukemia cell line.]]>58568571Zou, S., J.R. Carey, P. Liedo, D.K. Ingram and B. Yu,201234269279Choi, Y.K., S.G. Cho, H.S. Choi, S.M. Woo, Y.J. Yun, Y.C. Shin and S.G. Ko,2013Morus alba L. reduces the viability of multidrug-resistant MCF-7/Dox cells by inhibiting YB-1-dependent MDR1 expression.]]>2013Liang, L., X. Wu, M. Zhu, W. Zhao, F. Li, Y. Zou and L. Yang,20128215224Mudra, M., N. Ercan-Fang, L. Zhong, J. Furne and M. Levitt,20073012721274Sadeghi-Aliabadi, H., H. Mosavi, M. Mirian, S. Kakhki and A. Zarghi,20125558564Zhao, L., J.W. Pridgeon, J.J. Becnel, G.G. Clark and K.J. Linthicum,2014Aedes aegypti.]]>45401408Du, C., M. Fang, Y. Li, L. Li and X. Wang,20001023342Chen, Y.,1998135456Cai, W., X. Gu and J. Tang,2010Opuntia milpa alta skin.]]>28108116Helgason, C.D. and C.L. Miller,20043rd Edn.,Pages: 290Pages: 290Collins, J.A., C.A. Schandl, K.K. Young, J. Vesely and M.C. Willingham,199745923934Gercel-Taylor, C.,20052005pp: 79-82pp: 79-82Burton, K.,195662315323Bradford, M.M.,197672248254Mosmann, T.,1983655563Kollar, P., T. Barta, J. Hosek, K. Soucek and V.M. Zavalova et al.,2013Morus alba L. cause inhibition of G1/S transition in THP-1 human leukemia cells and prevent the lipopolysaccharide-induced inflammatory response.]]>2013Matsuo, M., N. Sasaki, K. Saga and T. Kaneko,200528253259Yadegarynia, S., A. Pham, A. Ng, D. Nguyen and T. Lialiutska et al.,2012712951304Enari, M., H. Sakahira, H. Yokoyama, K. Okawa, A. Iwamatsu and S. Nagata,19983914350Wang, I.K., S.Y. Lin-Shiau and J.K. Lin,19993515171525