Asian Journal of Biological Sciences1996-3351xxxx-xxxxScience International10.3923/ajbs.2013.340.346Toxoplasma gondii
in Eukaryotic Cell]]>KeshavarziFatemehAshtariParviz7201367T. gondii producing proteins are strong antigens that can start strong
immune reactions and one of these kinds of antigens is rhoptry protein 1 (ROP1)
that is discharged from rhoptry cell-organ. These entire attribute for ROP1
makes it a competitor for protein vaccine and recombining vaccine against toxoplasmosis.
A main objective of the current study was the Cloning and expression ROP1 of
Toxoplasma gondii in a cloning vector for later studies. In the present
work, genomic DNA of Toxoplasma gondii was removed and used for amplifying
of ROP1 gene as a template. Then PCR product was cloned into the EcoR1 and BamH1
sites of cloning vector, pUET1 and transformed into Escherichia coli
BL21 plysS strain and sub cloned from pTROP1 into the HindIII and EcoRI sites
of the pcDNA3 to produce recombining eukaryotic declaration vector pcROP1. The
cloned ROP1 was verified by PCR, limitation enzymes (HindIII and BglI) digestion
and nucleotide sequencing. A fragment about 757 bp was separated fore more nucleotide
sequence analysis of the ROP1 cloned in pUET1 vector revealed high homology
(96%) with RH strain Gene Bank Accession No. M71274. This expressed ROP1 to
make recombinant vaccine against toxoplasmosis will be useful in the future
study.]]>Beckers, C.J., J.F. Dubremetz, O. Mercereau-Puijalon and K.A. Joiner,1994Toxoplasma gondii rhoptry protein ROP 2 is inserted into the parasitophorous vacuole membrane, surrounding the intracellular parasite and is exposed to the host cell cytoplasm.]]>J. Cell Biol.,147947961Bhopale, G.M.,2003Micro. Infect.,5457462Fachado, A., A. Rodriguez, S.O. Angel, D.C. Pinto and I. Vila et al.,2003Vaccine,2113271335Gazzinelli, R.T., F.T. Hakim, S. Hieny, G.M. Shearer and A. Sher,1991Toxoplasma gondii vaccine.]]>J. Immunol.,146286292Herion, P., R. Hernandez-Pando, J.F. Dubremetz and R. Saavedra,1993Toxoplasma gondii.]]>J. Parasitol.,79216222Kimbita, E.N., X. Xuan, X. Huang, T. Miyazawa and S. Fukumoto et al.,2001Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1.]]>Vet. Parasitol.,1023544Leyva, R., P. Herion and R. Saavedra,2001Toxoplasma gondii.]]>Parasitol. Res.,877079Martin, V., A. Supanitsky, P.C. Echeverria, S. Litwin and T. Tanos et al.,2004Clin. Diagn. Lab. Immunol.,11704710McCabe, R. and J.S. Remington,1988N. Engl. J. Med.,318313315Perkins, M.E.,1992Parasitol. Today,82832Remington, J.S. and J.L. Krahenbuhl,1982Toxoplasma gondii.]]>Immunology of Toxoplasma gondii.1982pp: 327-371pp: 327-371Saavedra, R., F. De Meuter, J.L. Decourt and P. Herion,1991Toxoplasma gondii cloned and expressed in Escherichia coli.]]>J. Immunol.,14719751982Saavedra, R. and P. Herion,1991Toxoplasma gondii: Production of interferon-γ, interleukin-2 and strain cross-reactivity.]]>Parasitol. Res.,77379385Saffer, L.D., O. Mercereau-Puijalon, J.F. Dubremetz and J.D. Schwartzman,1992Toxoplasma gondii rhoptry protein by immunoelectron microscopy during and after host cell penetration.]]>J. Eukaryotic Microbiol.,39526530Sambrook, J., E.F. Fritsch and T. Maniatis,2001Molecular Cloning: A Laboratory Manual.3rd Edn.,Sambrook, J., E.F. Fritsch and T. Maniatis,1989Molecular Cloning: A Laboratory Manual.2nd Edn.,Pages: 25Pages: 25Suzuki, Y. and J.S. Remington,1988Toxoplasma gondii infection by Lyt-2+ and Lyt-1+, L3T4+ T cells in mice.]]>J. Immunol.,14039433946Wei, Q.K., D.B. Zhang, J. Li, G.P. Li, H.F. Wang and Y. Cui,2006Toxoplasma gondii.]]>Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi,24208211Wong, S.Y. and J.S. Remington,1994Clin. Infect. Dis.,18853861