Asian Journal of Biological Sciences1996-3351xxxx-xxxxScience International10.3923/ajbs.2012.76.85Salmonella spp. and Vibrio parahaemolyticus from Shrimp Samples of Bangladesh]]>FakruddinMd. IslamSumaiya AhmedMonzur Morshed ChowdhuryAbhijit HoqueMd. Mahfujul 2201252Lack of reliable and rapid method for detection of pathogens from shrimp in Bangladesh is an obstacle in ensuring the microbiological quality of the shrimp before export. Salmonella sp. and Vibrio parahaemolyticus are two potential pathogen for shrimp export value chain in Bangladesh. The objective of this study was to establish a multiplex polymerase chain reaction method for rapid and simultaneous detection of Salmonella spp. and Vibrio parahaemolyticus from export oriented shrimp samples. The targeted genes were tdh for V. parahaemolyticus and sefA for Salmonella spp. The genomic DNA was extracted and amplified for subsequent profiling. Validity of the multiplex PCR assay was tested by artificially inoculating the shrimp homogenate with viable cells of target pathogens. The genes were successfully amplified individually and simultaneously both from natural and spiked samples. Sensitivity of the assay was determined to be as low as 104 CFU mL-1. Amplification of DNA extracted from other bacterial pathogens viz. Bacillus cereus, Shigella flexneri and Staphylococcus aureus yielded negative results. This multiplex PCR assay will provide specific, rapid and reliable results and allow for the cost effective detection of target pathogens in a single reaction tube in mixed bacterial communities that are prevalent in shrimp products.]]>Ahari, H., D. Shahbazzadeh and A. Misaghi,2009SEA, SEB and SEC genes by multiplex PCR for rapid detection of Staphylococcus aureus.]]>812241228Ahmed, S. and M.N. Anwar,2007248184Akintola, S.L. and S.B. Bakare,2011Macrobrachium vollenhovenii, Herklots 1857) stored in ice.]]>6500506AlHaj, N., N.S. Mariana, A.R. Raha and Z. Ishak,2007E. coli and shiga toxin-producing E. coli by polymerase chain reaction.]]>725312534BBS,2010Elnifro, E.M., A.M. Ashshi, R.J. Cooper and P.E. Klapper,200013559570Fadel, H.M. and J. Ismail,2009Staphylococcus aureus and Enterobacteriaceae species in selected dairy products and handlers.]]>4100108Farag, N.S., A.A. Gomah and N.M.A. Balabel,201097378Hara-Kudo, Y., K. Sugiyama, M. Nishibuchi, A. Chowdhury and J. Yatsuyanagi et al.,2003Vibrio parahaemolyticus O3:K6 in seafood and the coastal environment in Japan. ]]>6938833891Brownie, J., S. Shawcross, J. Theaker, D. Whitcombe, R. Ferrie, C. Newton and S. Little,19972532353241Kawasaki, S., N. Horikoshi, Y. Okada, K. Takeshita, T. Sameshima and S. Kawamoto,2005Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7 in meat samples.]]>68551556Khan, A.W., S.J. Hossain and S.N. Uddin,2007Vibrio parahaemolyticus from shrimp at Khulna region of Bangladesh.]]>2216227Malkawi, H.I. and R. Gharaibeh,2004Salmonella enterica serotypes, enteritidis and typhimurium from chicken and meat products by multiplex PCR.]]>34448Martin, R.R., D. James and C.A. Levesque,200038207239Oliveira, S.D., C.R. Rodenbusch, M.C. Ce, S.L.S. Rocha and C.W. Canal,2003Salmonella detection.]]>36217221Ozdemir, Z.,2005Clavibacter michiganensis ssp. michiganensis and Xanthomonas axonopodis pv. vesicatoria.]]>4133137Parekh, T.S. and R. Subhash,200815663Porteen, K., R.K. Agarwal and K.N. Bhilegaonkar,2007Aeromonas sp. from chicken and fish samples by polymerase chain reaction.]]>23037Seidavi, A.R., S.Z. Mirhosseini, M. Shivazad, M. Chamani and A.A. Sadeghi,2008Salmonella sp., Escherichia coli and Bifidobacterium sp. in Chick gastrointestinal tract.]]>3230235Sherfi, S.A., I.E. Aradaib and H.A. Dirar,2006E. coli strains: A preliminary study.]]>1913Wilson, I.G.,19976337413751Wilson, K.,19871987pp: 241-245pp: 241-245Yasmin, M., S. Kawasaki and S. Kawamoto,2007Escherichia coli O157:H7, Listeria monocytogenes and Salmonella enteritidis in shrimp samples.]]>244246Sahasrabudhe, A. and M. Deodhar,2010Garcinia indica.]]>6293298