Asian Journal of Biological Sciences1996-3351xxxx-xxxxScience International10.3923/ajbs.2011.1.14Streptomyces rochei (MTCC 10109) Showing Antagonistic Activity Against Selective Human Pathogenic Microorganisms]]>ReddyN.G. RamakrishnaD.P.N. Raja GopalS.V. 1201141Marine antagonistic actinomycete strain Streptomyces rochei (MTCC 10109) was isolated from the sea water samples of Visakhapatnam coast of Bay of Bengal by serial dilution method. The aim of study was identification and characterization by its morphological, physiological and biochemical studies and deposited to Microbial Type Culture Collection and Gene bank, IMTECH, Chandigarh, India with accession number is MTCC 10109. It was a gram-positive, very long rods and filamentous organism, consisting of sporangia with spores observed by using cover-slip method and evaluated by phase contrast microscopes. It was capable to produce yellow pigments and possessing an earthy characteristic odour and exhibited optimum growth under aerobic conditions at temperature 30°C, pH at 7.0 and sodium chloride level at 2% (w/v). So, it was considered as a mesophillic, alkaliphillic and moderate salt tolerance in nature. The isolate showed difference in the carbon utilization, able to utilize all studied carbon sources, except xylose, adonitol, sorbitol, inositol and raffinose and showed positive results to methyl red test, nitrate reduction test, citrate utilization, urea hydrolysis, cytochrome oxidase, catalase test, gelatin hydrolysis, arginine dihydrolase, tween-60, tween-80 and esculin. It was exhibited broad antagonistic spectrum against all tested selective human pathogenic fungi and bacteria and its activity measured by zone of inhibition (mm), by agar well diffusion method. Among all the studied pathogens, Candida tropicana, Staphylococcus aureus and Escherichia coli were the most sensitive organisms from selective human test pathogens of fungi, gram-positive and gram-negative microorganisms respectively. Altogether, the results indicated that the natural marine environment is also good sources for isolation of novel varieties of antagonistic Streptomyces.]]>Alexander, M.,1961Mitra, A., S.C. Santra and J. Mukharjee,200880685695Atta, H.M. and M.S. Ahmad,2009Streptomyces sp. AZ-AR-262: Taxonomy, fermentation, purification and biological activities.]]>3126135Augustine, S.K., S.P. Bhavsar and B.P. Kapadnis,2005Streptomyces rochei AK 39.]]>121164170Baltz, R.H.,20088557563Getha, K., S. Vikineswary, W.H. Wong, T. Seki, A. Ward and M. Goodfellow,2004Streptomyces species and evaluation of their antifungal activity against selected plant pathogenic fungi.]]>233747Blunt, J.W., B.R. Copp, W. Hu, M.H.G. Munro, P.T. Northcote and M.R. Prinsep,2007243186Desphande, B.S., S.S. Ambedkar and J.G. Shewale,1988Streptomyces.]]>10455473Dhansasekaran, D., G. Rajakumar, P. Sivamani, S. Selvamani, A. Paneerselvam and N. Thanjuddin,2005118Donia, M. and M.T. Humann, 20033338348Ellaih, P. and A.P.C. Reddy,198716134135Fiedler, H.P., C. Bruntner, A.T. Bull, A.C. Ward and M. Goodfellow et al.,2005873742Goodfellow, M., Y. Kumar, D.P. Labeda and L. Sembiring,2007Streptomyces violaceusinger clade: A home for Streptomyces with rugose ornamented spores.]]>92173199Hakvag, S., E. Fjarvik, K.D. Josefsen, E. Ian, T.E. Ellingsen and S.B. Zotchev,2008Streptomyces spp. Isolated from the sea surface microlayer in the Trondheim Fjord, Norway.]]>6620635Morakchi, H., A. Ayari, M. Taok, D. Kirane and N. Cochet,2009Streptomyces strain SLO-105 isolated from Lake Oubeira sediments in North-East of Algeria.]]>863326336IMTECH,1998pp: 94pp: 94Saadoun, I. and A. Muhana,2008Streptomyces Ds-104 isolate against Multi-drug resistant Candia albicana.]]>2402420Jain, P.K. and P.C. Jain,2005Streptomyces purpoefuscus CM 1261.]]>43342345Jensen, P.R. and W. Fenical,199448559584Jensen, P.R., T.J. Mincer, P.G. Williams and W. Fenical,2005874348Kathresan, K., R. Balagurunathan and M. Selvam,20054271276Kavitha, M. and M. Vijayalakshmi,2007Streptomyces rochei.]]>320262029Kenneth, L.K.,195816427427Keiser, T., M.J. Bibb, M.J. Buttner, K.F. Chater and D.A. Hopwood,20002000pp: 121Kokare, C.R., K.R. Mahadik and S.S. Kadam,200433248256Kotake, C., T. Yamasaki, T. Moriyama, M. Shinoda and N. Komiyama et al.,19924514421450Kumar, Y. and M. Goodfellow,2008Streptomyces violaceusniger 16S rRNA gene clade: Streptomyces castelarensis sp. nov., comb. nov., Streptomyces himastatinicus sp. nov., Streptomyces mordarskii sp. nov., Streptomyces rapamycinicus sp. nov. and Streptomyces ruanii sp. nov.]]>5813691378Laidi, R.F., A. Elshafei, M. Sanker, B. Cheick and H. Hocine,20076489496Laidi, R.F., L.K. Amany, A.M. Elshafei and B. Cheikh,2006Streptomyces tendae.]]>9427436Laidi, R.F., M. Sifour, M. Sanker and H. Hocine,2008Staphylococcus aureus.]]>2422352241Larsen, H.,19863937Locci, R.,1989Streptomyces and Related Genera.]]>1989pp: 2451-2469pp: 2451-2469Sahu, M.K., K.S. Kumar and L. Kanan,2004244548Manivasagan, P., S. Gnanam, K. Sivakumar, T. Thangaradjou, S. Vijayalakshmi and T. Balasubramanian,2009Streptomyces sp. PM-32) isolated from an offshore sediments of the bay of bengal in tamilnadu.]]>3231236Mincer, T.J., P.R. Jensen, C.A. Kauffman and W. Fenical,20026850055011Miyadoh, S., 19939100106Naeimpoor, F. and F. Mavituna,2000Streptomyces coelicolor under various nutrient limitations.]]>2140148Narayana, J.K., P. Prabhakar, M. Vijayalakshmi, Y. Venkateswarlu and P.S. Krishna,2007Streptomycetes.]]>56191197Nonomura, H.,1974Streptomyces included in ISP.]]>527892 Odakura, Y., H. Kase, S. Itoh, S. Satoh and S. Takasawa et al.,1984Micromonospora olivasterospora.]]>3716701680Okamoto Hosay, Y., S. Okamoto and K. Ochi,2003Streptomyces lividans.]]>6942564259Oskay, A.M., T. Usame and A. Cem,20043441446Oskay, M.,2009Streptomyces strains.]]>830073017Pandey, B., P Ghimire and V.P. Agarwal,20042004pp: 14Pathiranana, C., D. Tapiolas, P.R. Jensen, R. Dwight and W. Fenical,19913223232326Pridham, T.G. and D. Gottlieb,194856107114Abdur Rahman, M.A., A.T.M. Zafrul Azam and M.A. Gafur,2000In vitro antibacterial principles of two flavonoids and extracts from Clerodendrum indicum linn.]]>317691771Sambamurthy, K. and P. Ellaiah,1974S. marinensis (part-1).]]>172428Sathi, Z.S., M.A.A. Rahman and M.A. Gafur,2001in vitro antimicrobial activity of compound isolated from Streptomyces speices.]]>415231525Talbot, G.H., J. Bradley, J.E. Edwards, Jr., D. Gilbert, M. Scheld and J.G. Bartlett,200642657668Tresner, H.D., J.A. Hayes and E.J. Backns,1968Streptomyces to sodium chloride as a taxonomic aid.]]>1611341136Ugur, A. and N. Sahin,2002Streptomyces species during growth with different nitrogen sources.]]>26171174Vijaykumar, R., C. Muthukumar, N. Thahuddin, A. Paneerselvam and R. Saravanamuthu,2007215965Vimal, V., R.B. Mercy and K. Kannabiran,2009Nocardiopsis sp. VITSVK 5 (FJ973467).]]>15763Ward, A.C. and M. Goodfellow,20042004pp: 288-313pp: 288-313Williams, S.T. and T. Cross,19711971pp: 295-334pp: 295-334You, J.L., L.X. Cao, G.F. Liu, S.N. Zhou, H.M. Tan and Y.C. Lin,2005Vibrio spp. from nearshore marine sediments.]]>21679682Zheng, Z., W. Zeng, Y. Huang, Z. Yang, J. Li, H. Cai and W. Su,20001888791Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley and S.T. Williams,20009th Edn.,pp: 189-255pp: 189-255Duggiri, G.D., 1976194491497Shirling, E.B. and D. Gottlieb,1966Streptomyces species.]]>16313340Singer, R.S., R. Finch, H.C. Wegener, R. Bywater, J. Walters and M. Lipsitch,200334751Sivakumar, K., M.K. Sahu, T. Thangaradjou and L. Kannan,200747186196Sultan, M.Z., N.A. Khatune, Z.S. Sathi, S.A.M.D. Bhuiyan and G.M. Sadik et al.,2002In vitro antibacterial activity of an active metabolite isolated from Streptomyces species.]]>1100106Kumari, K.S., K.U. Devi and A. Apparao,2009Streptomyces from Visakhapatnam coast.]]>17880Takizawa, M., R.R. Colwell and R.T. Hill,1993599971002